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Category Archives: Microbiology
Research – Efficacy of Power Ultrasound-Based Hurdle Technology on the Reduction of Bacterial Pathogens on Fresh Produce
Minimally processed produce is frequently contaminated with foodborne bacterial pathogens. Power ultrasound is a non-thermal and cost-effective technology that can be combined with other chemical sanitization methods. This study investigated the reduction of Listeria monocytogenes and Salmonella Newport on grape tomato, romaine lettuce, and spinach washed with water, chlorine, or peroxyacetic acid alone or in combination with 25 or 40 kHz power ultrasound for 1, 2, or 5 min. Produce items were inoculated with either pathogen at 10 log CFU/g, dried for 2 h, and treated. Combined treatment of ultrasound and sanitizers resulted in 1.44–3.99 log CFU/g reduction of L. monocytogenes and 1.35–3.62 log CFU/g reduction of S. Newport, with significantly higher reductions observed on grape tomato. Synergistic effects were achieved with the hurdle treatment of power ultrasound coupled with the chemical sanitizers when compared to the single treatments; an additional 0.48–1.40 log CFU/g reduction of S. Newport was obtained with the addition of power ultrasound on grape tomato. In general, no significant differences were observed in pathogen reductions between the ultrasound frequencies, the sanitizers, or the treatment lengths. Results from this study suggest that incorporation of power ultrasound into the current washing procedure may be beneficial for the reduction, but not elimination, of bacterial pathogens on certain produce items, including tomatoes.
Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Ultrasound
Norovirus outbreaks surging on cruise ships this year
Norovirus outbreaks spiked on cruise ships this year, with data showing more outbreaks happened between January and June than over the course of any other full calendar year in the last decade. Thirteen norovirus outbreaks have been reported on cruises so far in 2023, according to the U.S. Centers for Disease Control and Prevention, which keeps a record of annual case counts dating back to 1994.
The most recent outbreak hit passengers and crew members on board a Viking Cruises trip from Iceland that docked in New York on June 20. More than 13% of passengers on the Viking Neptune — 110 of 838 in total — reported being ill while onboard, according to the CDC. Nine crew members reported being ill as well. Health officials at the CDC determined that norovirus caused the outbreak after Viking Cruises collected and sent specimens to the agency’s laboratory for testing.
Scotland mulls pathogen action levels
Food Standards Scotland (FSS) is considering introducing thresholds for action for some pathogens based on either a UK or Scottish approach.
Using targets based on reported infections of key pathogens could help monitor trends and be used as a trigger to act. However, figures would need to be interpreted carefully because of underreporting, inability to separate food and non-food sources, changes in demographics, and impacts of year to year fluctuations in environmental factors, such as weather.
When reported cases breach the threshold, different steps would be considered including an investigation to identify reasons behind the increase; additional food sampling associated with the pathogen and risk communication to consumers using tailored messages.
FSS currently uses Public Health Scotland data on confirmed laboratory reports for five pathogens to monitor how foodborne illness is changing.
USA – Outbreak Investigation of Salmonella: Raw Cookie Dough (May 2023)
Product
Papa Murphy’s Take ‘N’ Bake raw Chocolate Chip Cookie and S’mores Bars dough purchased before May 23, 2023.
FDA’s investigation is complete and the outbreak is over.
Stores Affected
- Papa Murphy’s Stores Nationwide
Symptoms of Salmonella Infection
Illness usually occurs within 12 to 72 hours after eating contaminated food and usually lasts four to seven days. Symptoms include diarrhea, fever, and abdominal cramps. Children younger than five, the elderly, and people with weakened immune systems are more likely to have severe infections.
Status
Ended
Recommendation
- Papa Murphy’s Take ‘N’ Bake Chocolate Chip Cookie and S’mores Bars dough are not meant to be eaten raw.
- This outbreak appears to be over; however, consumers should check their refrigerators and freezers for Papa Murphy’s Chocolate Chip Cookie and S’mores Bars dough purchased before May 23, 2023 and should throw it away.
- Contact your healthcare provider if you think you may have symptoms of Salmonella infection.
Raw Dough Safety Tips
- Due to the potential for contamination, never eat any raw dough that is supposed to be cooked or baked.
- Always wash hands and clean and sanitize work surfaces and utensils thoroughly after contact with raw dough or batter.
- Keep raw food or dough separate from other foods while preparing them to prevent any possible contamination from spreading.
- More information is available on the FDA website.
Current Update
July 13, 2023
The FDA and CDC, in collaboration with state and local partners, investigated an outbreak of Salmonella Enteritidis infections linked to Chocolate Chip Cookie and S’mores Bars dough sold at Papa Murphy’s franchises and purchased before May 23, 2023.
FDA conducted a traceback investigation and identified two suppliers of interest. FDA and several state partners collected samples at both suppliers, and state partners also collected retail samples at several Papa Murphy’s stores. All samples collected were reported as negative for Salmonella contamination. As of July 13, 2023, CDC announced that the outbreak is over. CDC reports a total of 26 illnesses in six states. The last illness onset was May 22, 2023. FDA’s investigation is complete.
In response to this investigation, Papa Murphy’s notified franchise owners nationwide and stopped selling all Chocolate Chip Cookie dough and S’mores Bars dough at all stores and stopped using unprepared dry cookie dough mixes dating back through April 2023 to ensure any potentially contaminated ingredients are not used. As of July 13, 2023, Papa Murphy’s has not restarted sales of cookie dough. Additionally, the firm is reviewing their dessert menu items and labels to further enhance instructions to consumers to emphasize that their products are not intended to be eaten raw.
Posted in food bourne outbreak, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak, Salmonella
RASFF Alert – Animal Feed – Mold/Mould – Wheat Bran Pellets
Wheat bran pellets for animal feed with molds and moisture – Angola in Portugal
RASFF Alerts – Animal Feed – Salmonella – Rapeseed Extraction Meal
Salmonella spp. in rapeseed extraction meal (feed) from Germany in Spain and Switzerland
English Salmonella outbreak linked to eggs from Poland

A Salmonella outbreak in England that sickened more than 60 people earlier this year has been linked to eggs from Poland.
Samples from patients are also similar to previously reported isolates that fall into a cluster that is part of several national and international investigations.
There were 65 cases associated with the English outbreak; 25 confirmed and 18 probable infections linked to a restaurant, ten confirmed with unknown links to the establishment, and 12 with no link to the restaurant.
The outbreak linked to the unnamed restaurant was reported to the UK Health Security Agency (UKHSA) East of England Health Protection Team in early April. UKHSA was notified of multiple cases of gastroenteritis following food consumption at the site, with attendance or takeaway dates in late March, according to a study published in the journal Eurosurveillance.
Posted in food bourne outbreak, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak, Polish Chicken Salmonella, Salmonella, Salmonella in Eggs, Salmonella Poland
Denmark – Wrong expiration date on yogurt
Unifood Import A/S is recalling a batch of Gazi yoghurt due to an incorrect expiry date and thus risk of growth of micro-organisms. The product should be returned to the store where it was purchased or discarded
Gazi Yoghurt 3.5% (see picture)
Best before: 15 July 2023
The product is sold in: Specialty shops throughout the country
Research – Molecular and Genetic Characterization of Colicinogenic Escherichia coli Strains Active against Shiga Toxin-Producing Escherichia coli O157:H7
Abstract
The objective of this work was to molecularly and genotypically characterize and test the inhibitory activity of six colicinogenic Escherichia coli strains (ColEc) and their partially purified colicins against STEC O157:H7 isolated from clinical human cases. Inhibition tests demonstrated the activity of these strains and their colicins against STEC O157:H7. By PCR it was possible to detect colicins Ia, E7, and B and microcins M, H47, C7, and J25. By genome sequencing of two selected ColEc strains, it was possible to identify additional colicins such as E1 and Ib. No genes coding for stx1 and stx2 were detected after analyzing the genome sequence. The inhibitory activity of ColEc against STEC O157:H7 used as an indicator showed that colicins are potent growth inhibitors of E. coli O157:H7, being a potential alternative to reduce the presence of pathogens of public health relevance.
Posted in E.coli, E.coli O157, E.coli O157:H7, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, STEC, STEC E.coli
Multinational outbreak of Salmonella virchow ST16 infections associated with consumption of meat products containing chicken meat
Since June 2017, there is a persistent cross-border outbreak of Salmonella virchow ST16 to five countries in the European Union/European Economic Area (EU/EEA), the United Kingdom (UK) and the United States (US). .
A total of 210 cases have been reported to the following countries: Denmark (2), France (111), Germany (26), Ireland (4), the Netherlands (34), the United Kingdom (32) and the United States (1) . Hospitalization rates range from 16.7% (2/12) in the UK, to 29.4% (5/17) and 38.5% (10/26) in France and Germany, respectively.
No deaths have been reported. Most of the cases have been linked to local restaurants serving kebab meat. The number of confirmed cases represents only a small proportion of all infections in the EU/EEA, partly because of different genome sequencing (WGS) capacities by countries.
New infections are likely to occur in the EU/EEA affecting any age group, until further investigation is carried out to identify the sources and points of contamination along the chicken meat production chain.
Posted in food bourne outbreak, Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Foodborne Illness, foodborne outbreak, foodbourne outbreak, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, outbreak, Salmonella, Salmonella in Chicken


