Category Archives: microbial contamination

Research – Survival and Virulence of Listeria monocytogenes During Storage on Chocolate Liquor, Corn Flakes, and Dry-Roasted, Shelled Pistachios at 4ºC and 23ºC

JFP

The survival and virulence of Listeria monocytogenes was assessed during storage on three low-moisture foods (LMFs), chocolate liquor, corn flakes and shelled, dry-roasted pistachios (a w 0.18, 0.27, 0.20). The LMFs were inoculated with a 4-strain cocktail of L. monocytogenes at 8 log CFU/g, dried, equilibrated and then stored at 4°C, 25–81% relative humidity (RH) and 23°C, 30–35% RH for at least 336 days. At 4°C, L. monocytogenes remained stable on the LMFs for at least 336 days. At 23°C, L. monocytogenes levels declined on the chocolate liquor, corn flakes and pistachios at initial rates of 0.84, 0.88 and 0.32 log CFU/g/month, respectively. After 8 months at 23°C, L. monocytogenes concentrations on the chocolate liquor and corn flakes decreased to below the limit of detection (i.e., 0.48 log CFU/g). Relative populations of each strain were assessed before (i.e., day 0) and after 6 and 12 months of storage at 23°C and 4°C, respectively. Generally, a decline in the relative abundance of the serotype 1/2a strain was observed during storage, coupled with the relative increase of other strains, depending on the LMF and storage temperature. The total viable populations of L. monocytogenes quantified by PMAxx-qPCR after 12-plus months of storage at 4°C were significantly higher than that obtained by plating on TSA-YE by 1.8 to 3.7 logs. Decreases in the culturable population of L. monocytogenes during storage on the LMFs were the result of both cellular inactivation and transition to a viable-but-non-culturable state. The surviving cells, specifically after long-term storage at 4°C on the chocolate liquor and pistachios, remained infectious and capable of intracellular replication in Caco-2 enterocytes. These results have great relevance for predictive modeling used in microbial health risk assessments and support the addition of LMFs to food safety questionnaires conducted during listeriosis outbreaks.

RASFF Alert – Animal Feed -Aflatoxin – Groundnuts

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RASFF – aflatoxins (B1 = 25.4 µg/kg – ppb) in groundnuts for bird feed from India in the UK

RASFF Alert – Animal Feed – Salmonella – Processed Animal Proteins

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RASFF – Salmonella (in 1 out of 5 samples /25g) in processed animal proteins for petfood from Spain in Italy

Research – Survey of the U.S. Broiler Industry Regarding Pre- and Postharvest Interventions Targeted To Mitigate Campylobacter Contamination on Broiler Chicken Products

JFP

ABSTRACT

Campylobacter is one of the most commonly reported foodborne pathogens in the United States. Because poultry is considered a major source of Campylobacter infections in humans, reduction of Campylobacter contamination in poultry products is likely the most important and effective public health strategy for reducing the burden of campylobacteriosis in humans. A comprehensive on-line survey was conducted of key stakeholders in the U.S. broiler industry, including broiler farm managers (n = 18), poultry veterinarians (n = 18), and processing plant managers (n = 20), to assess the current pre- and postharvest Campylobacter interventions and control measures practiced by the industry for reducing Campylobacter contamination of broiler products. The survey also included information regarding each respondent’s understanding of Campylobacter transmission and ecology in relation to broiler production. The results revealed that a majority of the establishments included in the survey are following the U.S. Department of Agriculture, Food Safety and Inspection Service guidelines for controlling Campylobacter contamination in broiler flocks and on carcasses. However, establishments appeared to be putting more effort into Salmonella control than into Campylobacter control both on the farm and in the processing plant. A majority of the respondents responded that current interventions are not effective for reducing Campylobacter contamination, especially on the farm. Many respondents did not understand the risk factors associated with Campylobacter colonization in broiler flocks and on carcasses. Continued educational and training programs for key stakeholders in the U.S. broiler industry are needed to increase awareness of the issues associated with Campylobacter infection in broiler chickens and of the fact that Campylobacter infection is a multifaceted problem that requires efforts from both the pre- and postharvest sectors.

HIGHLIGHTS
  • Survey participants reported a lack of effective on-farm Campylobacter interventions.
  • Survey participants mostly adhered to government guidelines for reducing Campylobacter.
  • Survey participants differed in an understanding of Campylobacter transmission and ecology.
  • Better Campylobacter education programs are needed for poultry industry stakeholders.

Research – Foodborne Klebsiella pneumoniae: Virulence Potential, Antibiotic Resistance, and Risks to Food Safety

JFP

CDC Klebsiella

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ABSTRACT

Gastrointestinal carriage of Klebsiella pneumoniae is a predisposing factor for liver abscess in several Asian countries. To determine whether hypervirulent K. pneumoniae in the gut may be transmitted through food, we screened a range of raw and ready-to-eat retail food by culture and recovered K. pneumoniae in 21% (147 of 698) of samples tested. Based on PCR, no K. pneumoniae isolates carried the rmpA gene linked to community-acquired pyogenic liver abscess, providing no evidence of a link between food and liver disease. However, phenotypic resistance to multiple antibiotic classes was seen through disk diffusion tests, and carriage of genetic elements (wcaG and capsule types K1, K2, and K54) associated with increased virulence (8%, 11 of 147) was observed by PCR. Multidrug-resistant isolates were from raw vegetables, chicken or pork liver, and a ready-to-eat poultry dish; one multidrug-resistant K. pneumoniae isolate from raw bean sprouts was resistant to a third-generation cephalosporin (ceftriaxone). Although K. pneumoniae may be present in food without causing harm, we found isolates belonging to the K1 capsular serotype coexisting with the wcaG gene, one also conferring multidrug resistance. K. pneumoniae that carry antibiotic resistance genes, regardless of pathogenicity, may increase the available genetic pool of resistance along the food chain. Hygienic food handling practices are necessary to lower risks of acquiring K. pneumoniae and other opportunistic pathogens.

Research – Risk of Foodborne Illness from Pet Food: Assessing Pet Owners’ Knowledge, Behavior, and Risk Perception

JFP

Pet food has been identified as a source of pathogenic bacteria, including Salmonella and Escherichia coli. A recent outbreak linked to Salmonella -contaminated pet treats infected over 150 people in the United States. The mechanism by which contaminated pet food leads to human illness has not been explicated. Pet owners’ food safety knowledge and their pet food handling practices have not been reported. This study evaluated pet owners’ food safety knowledge and pet-food handling practices through an online consumer survey. The survey consists of 62 questions and assesses (1) owners’ food safety knowledge and pet-food handling practices; (2) owners’ interaction with pets; (3) owners’ risk perception related to their own health, their children’s health, and their pets’ health. The survey was pilot-tested among 59 pet owners before distribution to a national consumer panel, managed by Qualtrics XM. All participants (n=1,040) were dog and/or cat owners in the United States. Almost all pet owners interacted with their pets (93%) and most cuddled, allowed their pets to lick them, and slept with their pets. Less than one-third of pet owners washed their hands with soap after interacting with their pets. Over half (58%) the owners reported washing their hands after feeding their pets. Most pet owners fed their pets dry pet food and dry pet treats. Some fed their pets raw meat or raw animal product (RAP) diets because they believed these diets to be beneficial to their pet’s overall health. Many owners (78%) were unaware of pet food recalls or outbreaks associated with foodborne pathogens. Less than 25% considered dry pet foods and treats as a potential source of foodborne pathogens. The findings of this study indicated the need for consumer education about pet food handling. The data collected can assist in developing more accurate risk assessment models and consumer education related to pet food handling.

Australia -Five children hospitalized in backyard poultry Salmonella outbreak

Food Safety News

 

Five children needed hospital treatment in an Australian state as part of a Salmonella outbreak linked to backyard poultry.

Queensland Health is investigating Salmonella Typhimurium infections predominantly among young children that had contact with backyard chickens.

As of June 26, there had been 17 cases reported across the state. Thirteen of these were children aged 11 years or younger. Five were hospitalized because of their illnesses.

 

USA – Stay Food Safe this July Fourth

FSIS USDA  4th

Many Americans will be celebrating the Fourth of July outdoors this year a little differently, with celebrations at home, including backyard barbecues and picnics perhaps with only your household. No matter how you’re celebrating the Fourth of July, the U.S. Department of Agriculture’s (USDA) Food Safety and Inspection Service (FSIS) encourages you to make food safety and other public health recommendations a part of your celebration.

“Foodborne illness can increase during summer because of the warmer temperatures and extended time spent outside,” said Dr. Mindy Brashears, the USDA’s Under Secretary for Food Safety. “You may not be grilling at the park this year, but instead you may be grilling at home. As we celebrate this Fourth of July holiday, I encourage consumers to use food safety steps to reduce their risk of illness.”

Follow these tips from USDA to ensure a food safe Fourth of July:

Don’t Cross-Contaminate

Always keep raw meat and their juices from touching other foods. While grilling, avoid using the same utensils for cooked and ready-to-eat foods that were previously used with raw meat or poultry products. Wash and sanitize all surfaces and utensils after they touch raw items. A recent USDA survey showed that 34 percent of respondents do not follow an important step to use a different utensil to take food off the grill. Bring enough tools to keep your raw meat and poultry away from any cooked or ready-to-eat foods and have extra cleaning and sanitizing supplies ready for your surfaces, plates and utensils.

Use a Food Thermometer

Some grill masters may say they know their food is done just by looking at its color when it comes off the grill. That’s not possible and shouldn’t be relied upon. This is where a food thermometer comes in.

“More than 25 percent of burgers can turn brown inside before they are fully cooked,” says FSIS Administrator Paul Kiecker. “Although your grilled foods may look done, foodborne illness causing germs are not killed until the safe internal temperature has been reached. Using a food thermometer is the only way to know your food is done and safe to eat.”

The USDA recommended safe minimum internal temperatures are:

  • Beef, pork, lamb and veal (steaks, roasts and chops): 145°F then rest for three-minutes
  • Fish: 145°F
  • Ground meats (beef, pork, lamb and veal): 160°F
  • Whole poultry, poultry breasts and ground poultry: 165°F

Keep Foods at a Safe Temperature

Perishable food items should not be left outside for more than two hours, and only one hour if the temperature is at or above 90°F. Keep your food at or below 40°F, in coolers or containers with a cold source, such as ice or frozen gel packs. This includes any leftovers from the grill, cold salads and even cut fruits and vegetables. Leftovers should be refrigerated or placed back in the cooler within 2 hours of being placed outside (1 hour if temperatures are at or above 90°F). If you are not sure how long food has been sitting out, throw it out immediately.

If you have questions about these tips, or any other food safety topics, call the USDA Meat and Poultry Hotline at 1-888-MPHotline (1-888-674-6854) or chat live at ask.usda.gov from 10 a.m. to 6 p.m. Eastern Time, Monday through Friday.

RASFF Alert – Animal Feed – Enterobacteriaceae – Frozen Raw Pet Food

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RASFF – too high count of Enterobacteriaceae (between 3600 and 8400 CFU/g) in frozen raw pet food from Austria in Slovenia

RASFF Alerts – Animal Feed – Salmonella – Sunflower Meal

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RASFF – Salmonella enterica ser. Typhimurium (presence /25g) in sunflower seed meal from Romania in France