Monthly Archives: March 2018

Research – Cetylpyridinium chloride direct spray treatments reduce Salmonella on cantaloupe rough surfaces

Wiley Online Library Salmonella kswfoodworld

Abstract

Cetylpyridinium chloride (CPC) solutions (0, 0.5, or 1.0%) were applied to cantaloupe (“Athena” and “Hale’s Best Jumbo” cultivars) rind plugs, either before or after inoculation with a broth culture of Salmonella Michigan (109 CFU/mL) and held at 37°C for 1 or 24 hr. Rind plugs were diluted, shaken, and sonicated, and solutions were enumerated. Texture quality and color were evaluated over 14 days storage at 4°C after 0 and 1% CPC spray applications. A 0.5 or 1.0% (vol/vol) application of CPC after Salmonella reduced the pathogen levels between 2.34 log CFU/mL and 5.16 log CFU/mL in comparison to the control (p < .01). No differences were observed in the firmness and color of 1% CPC treated cantaloupes. Salmonella concentrations on cantaloupes, treated with 1.0% CPC, were lower after 1 hr storage as compared to 24 hr. And, Salmonella on “Athena” surfaces were more susceptible to CPC spray treatments than on “Hale’s Best Jumbo.”

Research – Biofilm‐forming ability of Alicyclobacillus spp. isolates from orange juice concentrate processing plant

Wiley Online

Abstract

The objective of this study was to evaluate the biofilm‐forming ability of Alicyclobacillus spp. isolates. Biofilm formation was evaluated under a stainless steel surface using orange juice as a culture medium at different temperatures (28 and 45  °C, 30 and 45  °C, and 45 and 60  °C) and contact times (24, 72, and 120 hr). The degree of hydrophobicity and the survival time of the different isolates on the abiotic surface were also investigated. Five Alicyclobacillus spp. isolates from the orange juice industry and a reference strain of Alicyclobacillus acidoterrestris 0244T were used. The biofilm formation of Alicyclobacillus was observed from 24 hr of contact in at least one temperature tested, as a function of the different incubation times and temperatures. Alicyclobacillus spp. presented variations in the degree of hydrophobicity. Surprisingly, two biofilm‐forming isolates (CCT 7230 and CCT 7346) were hydrophilic, with hydrophobicity index <20%, therefore, they are not necessarily related to cell hydrophobicity and adherence of Alicyclobacillus to the stainless steel surface. This study demonstrated that Alicycclobacillus spp. isolates from the industry can survive the processing conditions and form biofilms on contact surfaces.

Practical applications

Alicyclobacillus spp. may be present in the orange juice industry and form biofilms. In addition, it is known that the equipment is exposed to a wide temperature range for different periods of time and that poorly sanitized stainless steel equipment and surface can provide substrates for the formation of biofilms by Alicyclobacillus spp. Therefore, it is of the most importance to understand how these factors influence the adhesion and formation of Alicyclobacillus spp. in the processing of orange juice.

Research – Microorganisms can escape from a dead end by swimming

Science Daily 

 

Although microorganisms are too tiny to be seen by the naked eye, they are distributed all over the world and even inside the human body. It has been largely unknown how microorganisms can survive in intricate environments, such as in the ground and inside of the body, despite the importance of such information.


Takuji Ishikawa and Kenji Kikuchi from the Graduate School of Engineering at Tohoku University have discovered that microorganisms with hair like organelle called cilia, can avoid entrapment at a dead end, where cells would otherwise never return.

By using experiments and numerical simulations, Ishikawa and Kikuchi have shown that microorganisms can ingeniously escape from a dead end by swimming. The ability to escape from a dead end can be found in many kinds of microorganisms with cilia.

These results indicate that such a microorganism can find a way to survive even in intricate and challenging environments, where other creatures may become extinct, such as shallow water where a whale may become stuck. The results also pave the way to understanding the mechanism of the spread of infectious diseases.

This research was published online in Proceedings of the Royal Society B on 28 February 2018. It was financially supported by JSPS KAKENHI.

RASFF Alerts – Salmonella – Chilled Poultry Meat – Frozen Poultry Nuggets – Turmeric Powder – White Sesame Seeds – Black Pepper – Whole Wheat Flour – Chicken Half Breasts

kswfoodworld food safety poisoning

RASFF-Salmonella enterica ser. Typhimurium in chilled poultry meat from the Netherlands in the Netherlands

RASFF-Salmonella (presence /25g) in frozen poultry nuggets from Spain in France

RASFF-Salmonella enterica ser. Agona (present /25g) in turmeric powder from India, via the United Kingdom in Finland

RASFF-Salmonella (present /25g) in white sesame seeds from India in Cyprus

RASFF-Salmonella (present /25g) in whole wheat flour with roasted malt from Belgium in Belgium

RASFF-Salmonella (present /25g) in black pepper from Brazil in Spain

RASFF-Salmonella (presence /25g) in frozen salted chicken half breasts from Brazil in the Netherlands

Research – MMWR Examines Trends of Foodborne Illness Outbreaks From 2006 to 2017

Food Poisoning Bulletin 

 

The CDC is examining trends of foodborne illness outbreaks for 2017 and describes changes in incidence since 2006 in its Morbidity and Mortality Weekly Report (MMWR) for March 23, 2018. Foodborne illness is a substantial health burden in the Untied States. In 2017, there were 24,484 infections, 5,677 hospitalizations, and 122 deaths attributed to food borne illness.

Despite ongoing food safety measures in the United States, foodborne illness continues to be a substantial health burden. The 10 U.S. sites of the Foodborne Diseases Active Surveillance Network (FoodNet)* monitor cases of laboratory-diagnosed infections caused by nine pathogens transmitted commonly through food. This report summarizes preliminary 2017 data and describes changes in incidence since 2006. In 2017, FoodNet reported 24,484 infections, 5,677 hospitalizations, and 122 deaths. Compared with 2014–2016, the 2017 incidence of infections with Campylobacter, Listeria, non-O157 Shiga toxin–producing Escherichia coli (STEC), Yersinia, Vibrio, and Cyclospora increased. The increased incidences of pathogens for which testing was previously limited might have resulted from the increased use and sensitivity of culture-independent diagnostic tests (CIDTs), which can improve incidence estimates (1). Compared with 2006–2008, the 2017 incidence of infections with Salmonella serotypes Typhimurium and Heidelberg decreased, and the incidence of serotypes Javiana, Infantis, and Thompson increased. New regulatory requirements that include enhanced testing of poultry products for Salmonella might have contributed to the decreases. The incidence of STEC O157 infections during 2017 also decreased compared with 2006–2008, which parallels reductions in isolations from ground beef.§ The declines in two Salmonella serotypes and STEC O157 infections provide supportive evidence that targeted control measures are effective. The marked increases in infections caused by some Salmonella serotypes provide an opportunity to investigate food and nonfood sources of infection and to design specific interventions.

RASFF Alerts – Aflatoxin – Chopped Hazelnuts – Groundnut Kernels – Ground Nutmeg – Pistachios – Hazlenuts

kswfoodworld food safety poisoning

RASFF-aflatoxins (B1 = 60.09; Tot. = 71.48 µg/kg – ppb) in roasted and chopped hazelnuts from Turkey in Germany

RASFF-aflatoxins (B1 = 132 µg/kg – ppb) in groundnut kernels from China in Spain

RASFF-aflatoxins (B1 = 62; Tot. = 68 µg/kg – ppb) and ochratoxin A (230 µg/kg – ppb) in ground nutmeg from Lebanon

RASFF-aflatoxins (B1 = 12.8; Tot. = 13.6 µg/kg – ppb) in salted pistachios in shell from Turkey in Germany

RASFF-aflatoxins (B1 = 12.2 ± 3.6; Tot. = 13.5 ± 4.1 µg/kg – ppb) in hazelnuts shelled from Azerbaijan, via Georgia in Germany

RASFF-aflatoxins (B1 = >24; Tot. = >24 µg/kg – ppb) in pistachio nuts from the United States in Spain

RASFF-aflatoxins (B1 = 14.9; Tot. = 16.2 µg/kg – ppb) in hazelnut kernels from Azerbaijan, via Georgia in Germany

RASFF-aflatoxins (B1 = 11; Tot. = 61.1 µg/kg – ppb) in hazelnuts from Azerbaijan, via Georgia in Germany

Research – Staphylococcus aureus: A new mechanism involved in virulence and antibiotic resistance

Science Daily 

 

An Institut Pasteur-CNRS research team has characterized a Staphylococcus aureus gene involved in virulence, biofilm formation and resistance to certain antibiotics. These results open up new avenues for understanding the control of S. aureus virulence mechanisms. This work was recently published in the journal PLoS Pathogens.

Staphylococcus aureus is part of the natural skin flora, preferentially colonizing external mucosa in 30 to 50% of the population, healthy carriers who develop no symptoms. But it is also a major human pathogen, causing diseases ranging from skin lesions (boils, impetigo, etc.) to endocarditis, acute pneumonia, osteomyelitis or sepsis. It is the leading Gram-positive bacterium responsible for nosocomial infections (hospital acquired infections). The most dangerous strains are those that display resistance to multiple antibiotics. This is the case of MRSA[1], resistant to Meticillin, widespread in hospitals and posing a major public health concern.

RASFF Alert -STEC E.Coli – Soft Cheese

kswfoodworld food safety poisoning

RASFF-shigatoxin-producing Escherichia coli (130 CFU/g) in soft cheese from France in Norway

RASFF Alert- Ochratoxin – Ground Nutmeg

kswfoodworld food safety poisoning

RASFF-aflatoxins (B1 = 62; Tot. = 68 µg/kg – ppb) and ochratoxin A (230 µg/kg – ppb) in ground nutmeg from Lebanon in the Netherlands

RASFF Alerts – Norovirus – Live Oysters

kswfoodworld food safety poisoning

RASFF-norovirus in live oysters (Crassostrea gigas) from France, packaged in Italy  in Italy

RASFF-norovirus (GI and GII /2g) in live oysters from France in Denmark

RASFF-norovirus (GI /2g) in live oysters from France in Italy