Category Archives: Research

Research- New insights into what helps Salmonella cause infections

Science Daily

Salmonella is notorious for surviving and replicating in macrophages, which are normally lethal to invading bacteria because of their inhospitable environment. In a new study, researchers have discovered how a system of proteins, called TamAB, helps Salmonella survive under the harsh conditions inside macrophages.

Salmonella is a foodborne pathogen that causes more than a million infections each year in the U.S. Concerningly, it can kill young, old, and immunocompromised individuals.

What makes these bacteria especially dangerous is their ability to evade our immune responses. Read More at the Link Above

USA – Salmonella Outbreak Linked to Dry Dog Food

CDC

This outbreak investigation is over, but the recalled pet foods have long shelf lives. Check your home for the recalled pet food and don’t feed it to pets.

Fast Facts

Illnesses: 7

Hospitalizations: 1

Deaths: 0

States: 7

Recall: Yes

Investigation status: Closed   (first posted on November 9, 2023)

Mid America Pet Food

  • All dog and cat food
  • Best by dates before 10/31/2024
  • Brands include: Victor Super Premium Dog Foods, Wayne Feeds Dog Food, Eagle Mountain Pet food, Member’s Mark
  • Multiple bag sizes including: 5 pound, 15 pound, 16 pound, 30 pound, 40 pound, and 50 pound
  • See recall for more details

Victor brand Hi-Pro Plus dog food

  • 5-pound bags with lot code 1000016385
  • Best-by date 4/30/2024
  • See recall for more details

Victor brand Super Premium Dog Food, Select Beef Meal & Brown Rice Formula

  • 5 pound, 15 pound, and 40 pound bags
  • Best-by date 6/12/2024
  • Lot numbers 1000016890, 1000016891, 1000016892
  • See recall for more details

Research – ECDC – Giardiasis – Annual Epidemiological Report 2018

ECDC

In 2018, 21 049 confirmed giardiasis cases were reported in the EU/EEA. The EU/EEA notification rate was 5.6 cases per 100 000 population. The highest notification rates were reported in Belgium, Bulgaria and Sweden. The highest notification rate per 100 000 population was observed in the age group 0–4 years (17.2 for males and 14.4 for females). While the EU/EEA notification rate was stable from 2014 to 2018, the annual number of cases has increased steadily.

Research – Measures for the control of non-typhoidal Salmonella spp.in poultry meat

FAO

FAO & WHO. 2023. Measures for the control of non-typhoidal Salmonella spp. in poultry meat – Meeting report. Microbiological Risk Assessment Series, No. 45. Rome. https://doi.org/10.4060/cc9026e

Executive summary
Scope and objectives
In response to a request from the 52nd Session of the Codex Committee on Food
Hygiene (CCFH), the FAO/WHO Joint Expert Meetings on Microbiological
Risk Assessment (JEMRA) convened a meeting in Geneva, Switzerland from 12 to 16
September 2022, to collate and assess the most recent scientific information
relating to the control of non-typhoidal (NT)-Salmonella spp. in chicken meat,
including a review of the Codex Guidelines for the Control of Campylobacter
and Salmonella in Chicken Meat (CXG 78-2011). 1 The group of subject matter
experts reviewed the available data on NT Salmonella spp. control in the broiler
production chain, including scientific literature published since 2008 and
data submitted in response to a call for data for this meeting. The experts: 1)
determined the extent to which various control measures, good hygiene practices
(GHPs) or hazard-based control measures (targeted to reduce NT-Salmonella
spp.), provided adequate evidence for assessing their efficacy; 2) evaluated the
impact or efficacy of control measures relevant to NT-Salmonella spp. in the
broiler production chain, noting the variability of the impact reviewed and
recommended revisions to the Guidelines for the Control of Campylobacter and
Salmonella in Chicken Meat (CXG 78-2011), Paragraphs 1 to 114, based on the
evidence currently available (Annex 3).
Based on evaluation criteria such as the number, quality, applicability and
representativeness of reports and research on a particular intervention available
for screening, many control measures lacked sufficient evidence to allow the
experts to assess their effectivness.

Research Bio- Mapping Salmonella and Campylobacter Loads in Three Commercial Broiler Processing Facilities in the United States to Identify Strategic Intervention Points

MDPI

Abstract

The poultry industry in the United States is one of the largest in the world. Poultry consumption has significantly increase since the COVID-19 pandemic and is predicted to increase over 16% between 2021 and 2030. Two of the most significant causes of hospitalizations and death in the United States are highly related to poultry consumption. The FSIS regulates poultry processing, enforcing microbial performance standards based on Salmonella and Campylobacter prevalence in poultry processing establishments. This prevalence approach by itself is not a good indicator of food safety. More studies have shown that it is important to evaluate quantification along with prevalence, but there is not much information about poultry mapping using quantification and prevalence. In this study, enumeration and prevalence of Salmonella and Campylobacter were evaluated throughout the process at three different plants in the United States. Important locations were selected in this study to evaluate the effect of differences interventions. Even though there were high differences between the prevalences in the processes, some of the counts were not significantly different, and they were effective in maintaining pathogens at safe levels. Some of the results showed that the intervention and/or process were not well controlled, and they were not effective in controlling pathogens. This study shows that every plant environment is different, and every plant should be encouraged to implement a bio-mapping study. Quantification of pathogens leads to appropriate risk assessment, where physical and chemical interventions can be aimed at specific processing points with higher pathogen concentrations using different concentrations of overall process improvement.

Research – Multinational collaboration in solving a European Salmonella Braenderup outbreak linked to imported melons, 2021

Eurosurveillance

Multinational food-borne outbreak investigations require cross-sectoral collaboration not only at the local, regional and national level, but also across countries. This typically involves a number of organisations such as health authorities, regulatory food and veterinary authorities, central and regional laboratories as well as clinicians. Successful outbreak investigation is built on a coordinated approach at all regulatory and administrative levels. This paper presents an example of how a coordinated effort with cross-national collaboration was beneficial in solving a multinational outbreak of  Braenderup in Europe in the spring of 2021.

 Braenderup is a serovar of  subsp.  which causes symptoms of gastrointestinal illness including abdominal cramps, diarrhoea, nausea and fever [1]. . Braendrup ranked 19th among  serovars reported to the European Surveillance System (TESSy) between 2015 and 2019 and around 300 cases of  Braenderup in the EU/EEA are reported each year.

USA- Research – Investigations of Foodborne Outbreaks and Adverse Events in FDA-Regulated Foods

FDA

CORE 2022 Activities
65 Incidents Evaluated:
Incidents evaluated includes potential outbreaks, confirmed outbreaks, and adverse
events that were evaluated by the CORE Signals and Surveillance Team. Not all
incidents evaluated by Signals are transferred to Response for follow up.
28 Responses Initiated:
When an outbreak appears to be caused by an FDA-regulated food, this information is
passed to a Response Team to coordinate FDA’s response efforts.
11 Advisories Issued:
Public health advisories are issued to provide consumers with actionable advice about
an FDA-regulated food linked to an outbreak.

Research – Effectiveness of Ultra-High Irradiance Blue-Light-Emitting Diodes to Control Salmonella Contamination Adhered to Dry Stainless Steel Surfaces

MDPI

Controlling Salmonella contamination in dry food processing environments represents a significant challenge due to their tolerance to desiccation stress and enhanced thermal resistance. Blue light is emerging as a safer alternative to UV irradiation for surface decontamination. In the present study, the antimicrobial efficacy of ultra-high irradiance (UHI) blue light, generated by light-emitting diodes (LEDs) at wavelengths of 405 nm (841.6 mW/cm2) and 460 nm (614.9 mW/cm2), was evaluated against a five-serovar cocktail of Salmonella enterica dry cells on clean and soiled stainless steel (SS) surfaces. Inoculated coupons were subjected to blue light irradiation treatments at equivalent energy doses ranging from 221 to 1106 J/cm2. Wheat flour was used as a model food soil system. To determine the bactericidal mechanisms of blue light, the intracellular concentration of reactive oxygen species (ROS) in Salmonella cells and the temperature changes on SS surfaces were also measured. The treatment energy dose had a significant effect on Salmonella inactivation levels. On clean SS surfaces, the reduction in Salmonella counts ranged from 0.8 to 7.4 log CFU/cm2, while, on soiled coupons, the inactivation levels varied from 1.2 to 4.2 log CFU/cm2. Blue LED treatments triggered a significant generation of ROS within Salmonella cells, as well as a substantial temperature increase in SS surfaces. However, in the presence of organic matter, the oxidative stress in Salmonella cells declined significantly, and treatments with higher energy doses (>700 J/cm2) were required to uphold the antimicrobial effectiveness observed on clean SS. The mechanism of the bactericidal effect of UHI blue LED treatments is likely to be a combination of photothermal and photochemical effects. These results indicate that LEDs emitting UHI blue light could represent a novel cost- and time-effective alternative for controlling microbial contamination in dry food processing environments.

Research – Adhesion to and survival of foodborne pathogens in produce and strategies for their biocontrol

Wiley Online

Abstract

Foodborne pathogens can cause gastrointestinal infections in consumers and in some cases can even lead to outbreaks. In the last decade, it has been observed that some zoonotic pathogenic bacteria can use plants as secondary hosts. Contamination with foodborne bacteria becomes relevant in foods that are regularly eaten raw, such as lettuce, cilantro, fenugreek, rocket leaves, basil, and so forth, and some fruits such as tomatoes, melons, and green peppers; because the elimination of these pathogenic bacteria is difficult to achieve with conventional sanitization processes. Contamination of produce can occur throughout the entire production chain. In farmlands, pathogenic bacteria can contaminate the seed, mainly when contaminated water is used for irrigation. Later, bacteria can reach other plant tissues such as the stems, leaves, and fruits. Another form of contamination is when the produce is in contact with faeces from domestic, production, or wild animals. Additionally, poor handling practices during harvest, packaging, distribution, and sale can contaminate produce. Studies have shown that foodborne pathogens can adhere to produce, sometimes forming a biofilm, and can also be internalized into the plant or fruit, which protects them from sanitation processes. For this reason, in this text we address three biocontrol strategies such as bacteria, lytic bacteriophages, and some fungi, as an alternative approach for the control of both foodborne and plant pathogens. Additionally, the use of these biological agents can represent an advantage for the development of the plant, making them a good strategy to favour yield.

Research – Food Safety Aspects of Breeding Maize to Multi-Resistance against the Major (Fusarium graminearum, F. verticillioides, Aspergillus flavus) and Minor Toxigenic Fungi (Fusarium spp.) as Well as to Toxin Accumulation, Trends, and Solutions—A Review

MDPI

Abstract

Maize is the crop which is most commonly exposed to toxigenic fungi that produce many toxins that are harmful to humans and animals alike. Preharvest grain yield loss, preharvest toxin contamination (at harvest), and storage loss are estimated to be between 220 and 265 million metric tons. In the past ten years, the preharvest mycotoxin damage was stable or increased mainly in aflatoxin and fumonisins. The presence of multiple toxins is characteristic. The few breeding programs concentrate on one of the three main toxigenic fungi. About 90% of the experiments except AFB1 rarely test toxin contamination. As disease resistance and resistance to toxin contamination often differ in regard to F. graminearumF. verticillioides, and A. flavus and their toxins, it is not possible to make a food safety evaluation according to symptom severity alone. The inheritance of the resistance is polygenic, often mixed with epistatic and additive effects, but only a minor part of their phenotypic variation can be explained. All tests are made by a single inoculum (pure isolate or mixture). Genotype ranking differs between isolates and according to aggressiveness level; therefore, the reliability of such resistance data is often problematic. Silk channel inoculation often causes lower ear rot severity than we find in kernel resistance tests. These explain the slow progress and raise skepticism towards resistance breeding. On the other hand, during genetic research, several effective putative resistance genes were identified, and some overlapped with known QTLs. QTLs were identified as securing specific or general resistance to different toxicogenic species. Hybrids were identified with good disease and toxin resistance to the three toxigenic species. Resistance and toxin differences were often tenfold or higher, allowing for the introduction of the resistance and resistance to toxin accumulation tests in the variety testing and the evaluation of the food safety risks of the hybrids within 2–3 years. Beyond this, resistance breeding programs and genetic investigations (QTL-analyses, GWAM tests, etc.) can be improved. All other research may use it with success, where artificial inoculation is necessary. The multi-toxin data reveal more toxins than we can treat now. Their control is not solved. As limits for nonregulated toxins can be introduced, or the existing regulations can be made to be stricter, the research should start. We should mention that a higher resistance to F. verticillioides and A. flavus can be very useful to balance the detrimental effect of hotter and dryer seasons on aflatoxin and fumonisin contamination. This is a new aspect to secure food and feed safety under otherwise damaging climatic conditions. The more resistant hybrids are to the three main agents, the more likely we are to reduce the toxin losses mentioned by about 50% or higher.