Category Archives: Microbiology Investigations

Canadian Food Safety Agency implements temporary measures to address U.S. romaine lettuce – E.coli O157

Posted on August 1, 2023 by | Leave a comment

Food Safety News

To mitigate the risk associated with E. coli O157:H7 in romaine lettuce from the United States, the Canadian Food Inspection Agency (CFIA) has announced the implementation of temporary Safe Food for Canadians (SFC) license conditions.

The measures will be in effect from Sept. 28 to Dec. 20.

According to the CFIA, this action was taken to address the recurring outbreaks of foodborne illnesses related to E. coli O157:H7 in romaine lettuce imported from the United States. Investigations by U.S. authorities have traced the source of the outbreaks to the California Salinas Valley counties of Santa Cruz, Santa Clara, San Benito, and Monterey.

During the designated period, importers of romaine lettuce and salad mixes containing romaine lettuce from the U.S. must adhere to one of the following requirements:

  • Declare that the product does not originate from the Salinas Valley counties of California mentioned above, or
  • Submit an attestation form and Certificates of Analysis for each shipment, confirming that the romaine lettuce is free from detectable levels of E. coli O157:H7.

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Posted in CFIA, Decontamination Microbial, E.coli O157, E.coli O157:H7, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Safety, Food Safety Alert, Food Safety Management, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, STEC, STEC E.coli

Research – Salmonella spp., Escherichia coli and Enterobacteriaceae Control at a Pig Abattoir: Are We Missing Lairage Time Effect, Pig Skin, and Internal Carcass Surface Contamination?

Posted on August 1, 2023 by | Leave a comment

MDPI

Abstract

To provide meat safety and consumer protection, appropriate hygiene control measures at an abattoir are required. This study aimed to evaluate the influence of visual fecal contamination level (VFCL) and lairage time (LT) on pig skin (PS) and external (ECS) and internal (ICS) carcass surfaces. The presence of Enterobacteriaceae, Escherichia coli (E. coli) and Salmonella in PS, ECS, and ICS were evaluated. A total of 300 paired samples were collected from 100 pigs. Results underlined the importance of the skin (Enterobacteriaceae: 3.27 ± 0.68 log CFU/cm2; E. coli: 3.15 ± 0.63 log CFU/cm2; Salmonella: 21% of samples) as a direct or indirect source of carcass contamination. Although VFCL revealed no significant effect (p > 0.05), the increase of LT had a significant impact (p < 0.001) on Enterobacteriaceae and E. coli levels across all analysed surfaces, and Salmonella presence on ICS (p < 0.01), demanding attention to LT. Also, the ICS showed a higher level of these bacteria compared to ECS. These results highlight the need of food business operators to consider ICS as an alternative area to sample for Salmonella, as a criterion for process hygiene based on EC Regulation No. 2073/2005, and as a potential contamination source to be integrated in the hazard analysis critical control point (HACCP) plans.

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Posted in Decontamination Microbial, E.coli, Enterobacteriaceae, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Salmonella

Malta – Authorities probe reports of food poisoning following Kalkara wedding

Posted on August 1, 2023 by | Leave a comment

Times of Malta

The health authorities are investigating reports of people suffering from gastroenteritis following a wedding reception in Kalkara on Thursday, with at least one person hospitalised.

The Infectious Disease Prevention and Control Unit has asked attendees at the wedding reception at Villa Bighi to fill in an online form indicating whether they had fallen ill, what kind of symptoms they had, and when.

Attendees were also asked whether they had any complaints about the food served at the event because it may have tasted bad or was not warm enough, among other reasons.

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Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Poisoning, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Poisoning

USA – DPH warns residents about severe Vibrio infections caused by consumption of raw shellfish or exposure to salt or brackish water

Posted on July 31, 2023 by | Leave a comment

CT Portal

The Connecticut Department of Public Health (DPH) is warning residents about the potential dangers of consuming raw shellfish and exposure to salt or brackish water along Long Island Sound, due to severe Vibrio vulnificus infections.

 Since July 1, three cases of V. vulnificus infections have been reported to DPH. The three patients are between 60-80 years of age. All three patients were hospitalized and one died.  One patient reported consuming raw oysters from an out-of-state establishment. Two patients reported exposure to salt or brackish water in Long Island Sound. Both patients had pre-existing open cuts or wounds or sustained new wounds during these activities which likely led to the infections. 

 “The identification of these severe cases, including one fatality, due to V. vulnificus is concerning,” said DPH Commissioner Manisha Juthani, MD. “People should consider the potential risk of consuming raw oysters and exposure to salt or brackish water and take appropriate precautions. Particularly during the hottest months of the summer, bacteria are more likely to overgrow and contaminate raw shellfish. Given our current heat wave, this may be a time to exercise particular caution in what you consume.”

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Posted in Contaminated water, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Shellfish, Vibrio, Vibrio vulnificans, Vibrio vulnificus, Water, water microbiology, Water Safety

Ireland – Boil notice an ongoing risk for the immunocompromised in West Limerick -Cryptosporidium

Posted on July 31, 2023 by | Leave a comment

Limerick Post

LIMERICK residents who are at risk by being immunocompromised may be waiting months before they can shower in their own homes.

“Months” is how long Uisce Éireann says it may be until it can put a new treatment system in place to deal with cryptosporidium (a parasite found in human and animal faeces) found in the water supply at the Shannon Estuary Water Treatment Plant, which has seen a boil notice enforced in the area since May.

Affected households have been told that the water is safe to drink and to use to brush teeth if boiled, but medical experts warn that while infection can cause healthy people to become mildly ill, it can lead to a life-threatening illness for people with weak immune systems.

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Posted in Boil Water Notice, Contaminated water, Cryptosporidiosis, Cryptosporidium, Food Micro Blog, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Water, water microbiology, Water Safety

USA – Salmonella tainted Raw Milk sickens 5 children in Minnesota

Posted on July 31, 2023 by | Leave a comment

Food Poison Journal

The Minnesota Department of Health (MDH) is investigating a cluster of five illnesses caused by the bacteria Salmonella Typhimurium among children from the Twin Cities metro. The families of two children reported that they consumed unpasteurized milk. Information could not be obtained from the remaining families, but the bacteria from the cases were found to be identical through laboratory analysis. This indicates the infections came from the same source.

The cases include children ranging in age from 3 months to 10 years who became ill between the end of June and early July. One child was hospitalized.

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Posted in Food Illness, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Foodborne Illness, Illness, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Raw Milk, Salmonella

Canada reports nearly 200 non-travel related Cyclospora infections

Posted on July 31, 2023 by | Leave a comment

Outbreak News Today

The Public Health Agency of Canada (PHAC) reports investigating 185 non-travel related Cyclospora illnesses as of July 18, 2023.

170 cases, or 92 percent of the cases have been reported in Ontario with the other 15 cases reported from Quebec (9) and British Columbia (6).

Four people were hospitalized for their illnesses.

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Posted in Cyclospora, Cyclosporiasis, Food Micro Blog, Food Microbiology Blog, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Parasite, Protozoan

Research – Effect of Conventional Preservatives and Essential Oils on the Survival and Growth of Escherichia coli in Vegetable Sauces: A Comparative Study

Posted on July 30, 2023 by | Leave a comment

MDPI

Abstract

Essential oils have gained attention as natural alternatives to chemical preservatives in food preservation. However, more information is needed regarding consumer acceptance of essential oils in actual food products. This study aimed to compare the effects of conventional preservatives, heat treatment, and essential oils derived from thyme, oregano, and lemongrass on the survival and growth of pathogenic Escherichia coli in vegetable sauces. The results demonstrated a gradual decrease in pathogen numbers over time, even in untreated samples. On the fifth day of storage, heat treatment, sodium chloride, and acidification using citric acid (pH 3.2) exhibited reductions of 4.4 to 5.3 log CFU/g compared to the untreated control. Among the essential oils tested, lemongrass essential oil at a concentration of 512 mg/kg demonstrated the most remarkable effectiveness, resulting in a reduction of 1.9 log CFU/g compared to the control. Fifteen days after treatment, the control samples exhibited a contamination rate of 6.2 log CFU/g, while E. coli numbers in treated samples with heat, sodium chloride, citric acid (pH 3.2), and lemongrass essential oil (512 mg/kg) were below the detection limits. Additionally, sensory evaluation was conducted to assess the acceptability of the treated samples. The findings provide valuable insights into the potential utilisation of essential oils as natural preservatives in vegetable sauces and their impact on consumer acceptance.

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Posted in Decontamination Microbial, E.coli, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk

Research – Sanitizing Hatching Eggs with Essential Oils: Avian and Microbiological Safety

Posted on July 29, 2023 by | Leave a comment

MDPI

Abstract

Increased meat and egg production leads to concomitant changes in poultry practices, including the indiscriminate use of formaldehyde to sanitize hatching eggs. Although this sanitizer aids in the increase in poultry production, its toxic potential for man and for avian embryos represents an obstacle to its long-term use. This review assesses whether essential oils fit into the context of hatching egg contamination, reviewing their antimicrobial efficiency, toxicity to poultry embryos and chicks, and their sanitizing effects on poultry production parameters. Studies have indicated that, because they are safer, most of the essential oils studied can be a potential substitute for formaldehyde for minimizing microbial exposure of hatching eggs and embryos. However, complementary studies on the microbiological profile of embryos and chicks hatched from eggs sanitized with essential oils need to be carried out and the economic feasibility of the candidate products should also be considered.

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Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk

Research – Food Safety Monitoring of Salmonella spp. in Northern Italy 2019–2021

Posted on July 29, 2023 by | Leave a comment

MDPI

Abstract

Salmonella is the second most frequent bacterial pathogen involved in human gastrointestinal outbreaks in the European Union; it can enter the food-production chain from animal or environmental sources or from asymptomatic food operators. European food legislation has established microbiological criteria to ensure consumer protection. Salmonella is listed under both process hygiene criteria and food safety criteria. Each EU member state designates an agency to organize or perform controls and other official activities. This paper describes the official control plans performed by competent authorities in Northern Italy in the three-year period 2019–2021. A total of 4413 food samples were delivered to the IZS Food Safety laboratories for Salmonella detection, of which 36 (0.8%) tested positive. Salmonella was most frequently detected in poultry meat samples (25/36 positive samples) followed by other meat products and pork products. The official controls for the protection of consumer health apply the EU’s farm-to-fork approach: the samples were collected during production (food production plants), from products on the market, and from collective catering (restaurants, cafeterias, canteens). This manuscript will provide information about the presence of Salmonella in foodstuffs that can help competent authorities to set control plans based on risk assessments.

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Posted in Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Safety, Food Safety Management, food safety training, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Salmonella