Category Archives: Microbiological Risk Assessment

Research – A summary of cryptosporidiosis outbreaks reported in France and overseas departments, 2017–2020

Science Direct

water contamination

Abstract

Cryptosporidium is a known foodborne pathogen, ranked fifth out of 24 among foodborne parasites in terms of importance and a cause of many cryptosporidiosis outbreaks worldwide. In France, very few outbreaks were reported before 2017, and data recently obtained by the Expert Laboratory of the Cryptosporidiosis National Reference Center (CNR-LE-Cryptosporidiosis) have shown that outbreaks are in fact common and frequently underreported. In this work, we aim to report the characteristics of outbreaks detected in France during the period 2017–2020 and present a summary of investigations carried out by the CNR-LE-Cryptosporidiosis. During the study period, there were eleven cryptosporidiosis outbreaks, including three with no identified origin. Among the eight identified outbreaks: six were due to water contamination (five tap water and one recreational water), one was due to direct contact with infected calves, and one was due to consumption of contaminated curd cheese. Among these outbreaks, five of them exceeded one hundred cases. Recent results obtained by the CNR-LE-Cryptosporidiosis revealed the multiannual occurrence of Cryptosporidium outbreaks in France. Waterborne outbreaks were more frequently detected, while foodborne outbreaks which are more difficult to detect were likely underreported.

Research – Cross-Contamination to Surfaces in Consumer Kitchens Using MS2 as a Tracer Organism in Ground Turkey Patties

Journal of Food Protection

It is estimated that one in five cases of foodborne illnesses is acquired in the home. However, how pathogens move around a kitchen environment when consumers are preparing food is not well characterized. The purpose of this study was to determine the prevalence and degree of cross-contamination across a variety of kitchen surfaces during a consumer meal preparation event. Consumers (n=371) prepared a meal consisting of turkey patties containing the bacteriophage MS2 as a tracer organism and a ready-to-eat lettuce salad. Half were shown a video on proper thermometer use before the trial. After meal preparation, environmental sampling and detection were performed to assess cross-contamination with MS2. For most surfaces, positivity did not exceed 20%, with the exception of spice containers, for which 48% of the samples showed evidence of MS2 cross-contamination. Spice containers also had the highest MS2 concentrations, at a mean exceeding 6 log 10 viral genome equivalent copies (GEC) per surface. The high level of MS2 on spice containers drove the significant differences between surfaces, suggesting the significance of spice containers as a vehicle for cross-contamination, despite the absence of previous reports to this effect. The thermometer safety intervention did not affect cross-contamination. The efficiency of MS2 transfer, when expressed as a percentage, was relatively low, ranging from an average of 0.002 to 0.07%. Quantitative risk assessment work using these data would aid in further understanding the significance of cross-contamination frequency and efficiency. Overall, these data will help create more targeted consumer messaging to better influence consumer cross-contamination behaviors.

Research – The Impact of Thyme, Rosemary and Basil Extracts on the Chemical, Sensory and Microbiological Quality of Vacuumed Packed Mackerel Balls

MDPI

The effect of natural extracts (0.05%) and vacuum packaging on the sensory, chemical, and microbiological quality of mackerel balls were evaluated at refrigerated (4 ± 2 °C) storage. Natural extracts thyme (38.13 mg GAE/g), rosemary (81.85 mg GAE/g) and basil (21.08 mg GAE/g) were evaluated. Natural extracts imparted stability to lipids (TBA, FFA, and PV), and the ability was further improved by vacuum packaging. Biochemical changes (TVB-N, pH) and microbiological quality (total viable count) were also retained. Control samples packed under vacuum were found to cross over acceptable limits on day 28. Based on sensory quality evaluation, samples treated with rosemary and thyme extracts showed superior sensory quality over control, whilebasil-treated samples were not found acceptable at day 28. Consequently, the inclusion of thyme and rosemary extracts exhibits preservative quality when combined with vacuum packaging, retaining biochemical, microbial, and sensory quality.

Research – Genetic Diversity and Potential Virulence of Listeria monocytogenes Isolates Originating from Polish Artisanal Cheeses

MDPI

Artisanal cheeses can be sources of Listeria monocytogenes and cause disease in humans. This bacterial pathogen is a species of diverse genotypic and phenotypic characteristics. The aim of the study was to characterize 32 isolates of L. monocytogenes isolated in 2014–2018 from artisanal cheeses. The isolates were characterized using whole genome sequencing and bioinformatics analysis. The artisanal cheese isolates resolved to four molecular groups: 46.9% of them to IIa (1/2a-3a), 31.2% to IVb (4ab-4b-4d-4e), 12.5% to IIc (1/2c-3c), and 9.4% to IIb (1/2b-3b-7). Two evolutionary lineages emerged: lineage II having 59.4% of the isolates and lineage I having 40.6%. The sequence types (ST) totaled 18: ST6 (15.6% of the isolates), ST2, ST20, ST26, and ST199 (each 9.4%), ST7 and ST9 (each 6.3%), and ST1, ST3, ST8, ST16, ST87, ST91, ST121, ST122, ST195, ST217, and ST580 (each 3.1%). There were 15 detected clonal complexes (CC): CC6 (15.6% of isolates), CC9 (12.5%), CC2, CC20, CC26, and CC199 (each 9.4%), CC7 and CC8 (each 6.3%), and CC1, CC3, CC14, CC87, CC121, CC195, and CC217 (each 3.1%). The isolates were varied in their virulence genes and the differences concerned: inlactA, LIPI-3, amigtcAautvip, and lntAView Full-Text

Malaysia – 900 victims of Food Poisoning were reported in the first eight months of this year in Perak

The Sun Daily

Some 23 food poisoning cases involving 900 victims were reported in the first eight months of this year in Perak, said State Health, Science, Environment and Green Technology Committee chairman Mohd Akmal Kamarudin.

He said the Ministry of Health (MOH) is investigating the cause of the death of a food handler at a MOH training centre in Taiping three weeks ago to determine whether it was due to food poisoning or other illnesses.

According to him, so far there have been six incidents of food poisoning in schools (169 cases), public institutions (eight cases with 572 victims), factories (two cases with 66 victims), private homes (five cases with 24 victims), prison (one case with 22 victims) and external caterer (one case).

USA – Food Poisoning Outbreak in McHenry County, IL at D. C. Cobb’s

Food Poisoning Bulletin

A food poisoning outbreak in McHenry County, Illinois at D. C. Cobb’s restaurant has sickened at least 13 people, according to the McHenry County Health Department. That restaurant is located at 1204 North Green Street in McHenry.

Food Poisoning Outbreak in McHenry County, IL at D. C. Cobb’s

A food poisoning outbreak in McHenry County, Illinois at D. C. Cobb’s restaurant has sickened at least 13 people, according to the McHenry County Health Department. That restaurant is located at 1204 North Green Street in McHenry.

The outbreak was identified when public health officials reviewed complaints of people who got sick after eating at that establishment. An investigation into the pathogen that caused these illnesses, and the possible source of the pathogen has been launched.

USA – FDA -Seafood Safety -What’s New?

FDA

What’s New

Guidance for Industry

Publication of updated sections of the Fish and Fishery Products-Hazards and Controls Guidance.

RASFF Alerts – Animal Feed – Salmonella – Feather Meal – Soy Flour – Soya Bean Meal

RASFF

Salmonella in feather meal from Russia in Poland and Hungary

RASFF

Salmonella Senftenberg in soy flour from Hungary, via the Netherlands in Italy, Slovenia and Belgium

RASFF

Salmonella Yoruba in Soya bean meal from NL in Finland

India – At least 100 persons fall ill in Manipur; food poisoning suspected

Hindustan Times

Over 100 persons, including children, were hospitalised in Manipur’s Senapati district since Tuesday over suspected case of food poisoning from a birthday celebration in the hilly state.

The doctors said they are suspecting food poisoning. Locals claim the consumption of fermented fish chutney during birthday celebrations could be the primary cause.

“However, it will take at least 7 to 14 days to get the results for the samples which we collected and sent for testing and we’ve also collected blood samples to check other diseases, Dr Veidai said.”

Meanwhile, the food safety department officials also went to the Senapati district village to ascertain the causes of the mass sickness.

USA – FDA Core Outbreak Table Update

FDA

What’s New

  • A new outbreak of Listeria monocytogenes (ref# 1106) in a not yet identified product has been added to the table. FDA has initiated an on-site inspection and sample collection and analysis.
  • For the Salmonella Mississippi outbreak (ref# 1097) in a not yet identified product the case count has increased from 100 to 102 cases.
  • To highlight ongoing investigations, all incidents with a closed investigation and an outbreak that has ended will now be archived, by year, in the tables located below the Active Investigations table.