Category Archives: Zoonosis

Research – Efficacy of organic acids, lactic and formic acid, and peracetic acid in decontaminating process water and carcasses in chicken slaughter

Wiley Online

Abstract

Foodborne pathogens pose a persistent threat in broiler chicken production, particularly during the slaughter process, where contamination with zoonotic pathogens remains a concern. This study focuses on the potential of organic acids, such as formic and lactic acid, and the oxidizing agent peracetic acid, to decontaminate scalding water and enhance the hygiene of chicken carcasses. We conducted suspension tests introducing various organic loads to mirror the conditions of practical scalding water. Additionally, the surface tests were performed on chicken skin. Both methods were further tested in an experimental slaughtering facility. In suspension tests, the organic acids achieved impressive decontamination, with a 5-log10 reduction of the test organisms Enterococcus hiraeSalmonella Typhimurium and Campylobacter jejuni at minimal concentrations (between 0.04% and 2% for formic acid; between 0.1% and 4.5% for lactic acid). Peracetic acid also effectively sanitized model water and chicken skin, even when used in low concentrations (between 0.001% and 0.1%), both in the laboratory-based testing and in the experimental slaughtering facility. These results suggest that the tested disinfectants can effectively sanitize process water, even under conditions mimicking practical scalding water with organic matter. Peracetic acid, in particular, proved highly effective in improving chicken skin hygiene even at low concentrations.

Research – Genetic Diversity in Salmonella enterica in Outbreaks of Foodborne and Zoonotic Origin in the USA in 2006–2017

MDPI

Abstract

Whole genome sequencing is replacing traditional laboratory surveillance methods as the primary tool to track and characterize clusters and outbreaks of the foodborne and zoonotic pathogen Salmonella enterica (S. enterica). In this study, 438 S. enterica isolates representing 35 serovars and 13 broad vehicle categories from one hundred epidemiologically confirmed outbreaks were evaluated for genetic variation to develop epidemiologically relevant interpretation guidelines for Salmonella disease cluster detection. The Illumina sequences were analyzed by core genome multi-locus sequence typing (cgMLST) and screened for antimicrobial resistance (AR) determinants and plasmids. Ninety-three of the one hundred outbreaks exhibited a close allele range (less than 10 allele differences with a subset closer than 5). The remaining seven outbreaks showed increased variation, of which three were considered polyclonal. A total of 16 and 28 outbreaks, respectively, showed variations in the AR and plasmid profiles. The serovars Newport and I 4,[5],12:i:-, as well as the zoonotic and poultry product vehicles, were overrepresented among the outbreaks, showing increased variation. A close allele range in cgMLST profiles can be considered a reliable proxy for epidemiological relatedness for the vast majority of S. enterica outbreak investigations. Variations associated with mobile elements happen relatively frequently during outbreaks and could be reflective of changing selective pressures.

Research – Joint FAO/WHO Expert Meeting on microbiological risk assessment of viruses in foods Part 2: prevention and intervention measures – Summary

FAO

In the Part 1 Expert meeting, the virus-commodity combinations ranked of highest priority were human norovirus and hepatitis A virus in shellfish, fresh and frozen produce, prepared and ready-to-eat (RTE) foods, and hepatitis E virus in pork and wild game.

The Part 2 Expert meeting focused on these virus- commodity combinations and their associated contamination routes. Human faecal matter and vomit from infected individuals are the primary sources of contamination for norovirus and hepatitis A virus.
Across the food supply chain, the primary contamination routes are faecally-impacted waters, food handlers carrying foodborne viruses, and surfaces. Zoonotic hepatitis E virus is present in the meat, organ tissues, and excretions of infected swine and some game animals. Since that initial expert meeting report from 2008, awareness of the public health importance of these foodborne virus-commodity combinations has increased, resulting in additions or changes to some food supply chain management strategies and
research initiatives. Prevention remains the cornerstone of control of foodborne viruses. This is because these viruses are environmentally persistent and resistant to many treatments commonly used to inactivate foodborne pathogens. Effective inactivation methods continue to be necessary and are currently being evaluated.

Research – Campylobacter presence on Dutch broiler farms and associated risk factors

Science Direct

ABSTRACT

Campylobacter is the most reported zoonotic pathogen in humans in the European Union. Poultry is a major source of human infection with Campylobacter. Although many studies are done on the presence of Campylobacter in broilers and theoretically effective control measures are known, their relative importance at broiler farms remains poorly understood. Therefore the aim of this study was to investigate the presence of Campylobacter on selected broiler farms in the Netherlands, to determine the moment of introduction, and associated risk factors. A longitudinal study on 25 broiler farms was carried out between June 2017 and December 2020. Fecal samples were collected weekly from 43 broiler houses. In total 497 flocks were sampled. Putative variables on flock and farm characteristics for a risk factor analysis were gathered through questionnaires. Risk factors associated with the presence of Campylobacter in a broiler flock were determined using regression models. In total 30% of the flocks included in the study were positive for Campylobacter. Factors associated with presence of Campylobacter at slaughter age included: season, mowing lawns and presence of agricultural side activities. While summer/autumn and mowing lawns were associated with an increase in Campylobacter presence in flocks, the farmer having agricultural side activities other than poultry production was associated with a decrease. Analysis of the age at which flocks first tested Campylobacter positive revealed that slower growing breeds became positive on average one week later compared to regular growers. This study revealed a delayed introduction of Campylobacter in slower grower versus regular grower broiler flocks reared indoors. In addition it confirmed importance of season as major risk factor. The relevance of mowing and preceding positive flocks as risk factors needs further investigation.

EFSA – Zoonoses, foodborne outbreaks and antimicrobial resistance guidance for reporting 2023 data

EFSA

This technical report provides guidance for reporting countries on how to submit their isolate‐based quantitative antimicrobial resistance data, and data on the prevalence of zoonoses and microbiological agents and contaminants in food, food‐borne outbreaks, animal populations and disease status. To collect these data, EFSA has created the Data Collection Framework (DCF) application. This report includes data dictionaries to guide the reporting of information derived from 2023 under Directive 2003/99/EC, Regulation (EU) 2017/625, Commission Implementing Regulation (EU) 2019/627 and Commission Implementing Decision (EU) 2020/1729. The objective is to explain in detail the individual data elements of the EFSA data models that are to be used for XML data submission through the DCF. In particular, it explains the data elements to be reported, including information about the data type, a reference to the list of permitted terms and any additional business rules or requirements that may apply.

EU – Zoonoses, foodborne outbreaks and antimicrobial resistance guidance for reporting 2023 data

EFSA

This technical report provides guidance for reporting countries on how to submit their isolate-based quantitative antimicrobial resistance data, and data on the prevalence of zoonoses and microbiological agents and contaminants in food, food-borne outbreaks, animal populations and disease status. To collect these data, EFSA has created the Data Collection Framework (DCF) application. This report includes data dictionaries to guide the reporting of information derived from 2023 under Directive 2003/99/EC, Regulation (EU) 2017/625, Commission Implementing Regulation (EU) 2019/627 and Commission Implementing Decision (EU) 2020/1729. The objective is to explain in detail the individual data elements of the EFSA data models that are to be used for XML data submission through the DCF. In particular, it explains the data elements to be reported, including information about the data type, a reference to the list of permitted terms and any additional business rules or requirements that may apply.

Research – Adhesion to and survival of foodborne pathogens in produce and strategies for their biocontrol

Wiley Online

Abstract

Foodborne pathogens can cause gastrointestinal infections in consumers and in some cases can even lead to outbreaks. In the last decade, it has been observed that some zoonotic pathogenic bacteria can use plants as secondary hosts. Contamination with foodborne bacteria becomes relevant in foods that are regularly eaten raw, such as lettuce, cilantro, fenugreek, rocket leaves, basil, and so forth, and some fruits such as tomatoes, melons, and green peppers; because the elimination of these pathogenic bacteria is difficult to achieve with conventional sanitization processes. Contamination of produce can occur throughout the entire production chain. In farmlands, pathogenic bacteria can contaminate the seed, mainly when contaminated water is used for irrigation. Later, bacteria can reach other plant tissues such as the stems, leaves, and fruits. Another form of contamination is when the produce is in contact with faeces from domestic, production, or wild animals. Additionally, poor handling practices during harvest, packaging, distribution, and sale can contaminate produce. Studies have shown that foodborne pathogens can adhere to produce, sometimes forming a biofilm, and can also be internalized into the plant or fruit, which protects them from sanitation processes. For this reason, in this text we address three biocontrol strategies such as bacteria, lytic bacteriophages, and some fungi, as an alternative approach for the control of both foodborne and plant pathogens. Additionally, the use of these biological agents can represent an advantage for the development of the plant, making them a good strategy to favour yield.

Research-Contamination of Plant Foods with Bacillus cereus in a Province and Analysis of Its Traceability

MDPI

Abstract

Bacillus cereus is an important zoonotic foodborne conditional pathogen. It is found in vegetables, dairy products, rice, and other foods, thereby greatly endangering human health. Investigations on B. cereus contamination in China primarily focus on raw milk, dairy products, meat, and others, and limited research has been conducted on plant-based foodstuffs. The rapid development of sequencing technology and the application of bioinformatics-related techniques means that analysis based on whole-genome sequencing has become an important tool for the molecular-epidemiology investigation of B. cereus. In this study, we investigated the contamination of B. cereus in six types of commercially available plant foods from eight regions of a province. The molecular epidemiology of the isolated B. cereus was analyzed by whole-genome sequencing. We aimed to provide fundamental data for the surveillance and epidemiology analysis of B. cereus in food products in China. The rapid traceability system of B. cereus established in this study can provide a basis for rapid molecular epidemiology analysis of B. cereus, as well as for the prevention and surveillance of B. cereus. Moreover, it can also be expanded to monitoring and rapid tracing of more foodborne pathogens.

Germany -Meat of wild game animals should become safer

BFR

European network aims to further reduce health risks

Game meat, including those of red deer, wild boar, or pheasant, is among the foods with the smallest ecological footprint. These animals grow up in the wild, feeding on what na-ture provides, which also means that they can be exposed to various environmental con-taminants. Furthermore, wild animals can serve as carriers of zoonotic pathogens. The Eu-ropean network ‘Safety in the Game Meat Chain’ that will be established over the next four years under the leadership of the German Federal Institute for Risk Assessment (BfR),and promotes the exchange of knowledge regarding the health risks associated with game meat obtained through hunting for consumers. ‘Our goal is to make this valuable animal-derived food as safe as possible both in Europe and worldwide,’ says BfR President Professor Dr.Dr. Andreas Hensel. ‘We aim to minimize both chemical and microbial risks as much as possi-ble.’ The growing network currently includes 29 countries, encompassing not only EU member states but also non-EU countries, including those in the Western Balkans, Turkey, the United Kingdom, the United States, New Zealand, and Australia.

Over a four-year period, the five working groups of the network will focus on hunting and processing, game meat trade networks and the supply chain, as well as various biological and chemical hazards. To accomplish this, research activities from national level will be compiled and evaluated. Stakeholder groups from academia, industry, governmental institutions, and final consumers will receive ongoing updates on the new findings.

A central aim of the network is direct collaboration with stakeholder groups to translate knowledge into actions along the entire production chain, “from forest to fork” . The focus lies not only on undesirable substances from the environment (environmental con-taminants), but also on preventing or reducing the input of heavy metals, especially lead, from hunting ammunition. The biological hazards range from parasites such as Trichinella larvae, which can be transmitted to humans through the consumption of game meat, all the way to bacterial zoonotic agents including Salmonella and verotoxigenic Escherichia coli (VTEC), as well as viruses like hepatitis E (HEV) in wild boars.

Risks of both chemical and microbial origin that may arise during processing and trade of game meat, potentially contaminating the end product, will also be assessed. The goal is to disseminate scientific knowledge gained through the network from various regions and to align long-term food safety standards across borders.

The network also aims to gather insights into different hunting and training practices as well as national legal regulations and standards on meat inspection and hygiene for game meat in each country. The safety standards for cross-border trade in wild products are intended to be enhanced and harmonized. Additionally, the network places emphasis on educating con-sumers about the risks associated with and safe handling of game meat. Ultimately, this can support the consumer’s confidence in game meat products.

‘Safety in the Game Meat Chain’ is founded by the European Cooperation in Science and Technology (COST).

Useful links

SafeGameMeat COST Action (CA22166):
https://www.cost.eu/actions/CA22166/

Further information from the BfR website on game meat (in German)

Switzerland – Monitoring of Zoonoses

BLV

Current

zoonotic transverse

Publication of the Swiss Zoonoses Report 2022

In 2022, the number of reported zoonoses in humans generally increased compared to the two previous years. There were about the same number of cases as in 2019 before the Covid pandemic. The most frequently recorded zoonoses in humans were campylobacteriosis and salmonellosis. In both diseases, contaminated food is the main source of the disease. Consumers can reduce the risk of infection with good kitchen hygiene and simple measures: seesichergeniessen.ch