Category Archives: Staphylococcus aureus

Research – Effect of Gum Arabic‐polyvinyl alcohol films containing seed cover extract of Zanthoxylum rhetsa on shelf life of refrigerated ground chicken meat

Wiley Online

Abstract

The aim of this study was to improve shelf life of ground chicken meat in chilled storage by employing active packaging incorporating seed cover extract (SCE) of Zanthoxylum rhetsa. The total phenolic and flavonoid content in SCE was estimated to be 194 and 44.5 mg/g, respectively, in terms of catechin equivalents. SCE scavenged free radicals, exhibited good reducing power, and high antioxidant activity coefficient. SCE was effective against Staphylococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas fluorescens. The major phenolics compounds identified in SCE were catechin, vanillin, gallic acid, and ferulic acid. SCE was incorporated to get active films of Gum Arabic, polyvinyl alcohol, and sorbitol. Films had good mechanical and barrier properties. Ground chicken meat packed in normal films spoiled in less than 6 days whereas active packaging led to reduction in lipid peroxidation and improvement in shelf life up to 12 days at chilled condition.

Practical applications

Globally, tonnes of food get wasted annually at retail and consumer level due to lack of proper packaging and storage facilities. Meat is a highly perishable commodity and various strategies are used to preserve it for long time. Packaging and freezing are two important techniques used for this purpose. Freezing is energy intensive and conventional packaging using petroleum‐based polymers leads to burden on environment. Active packaging with the use of biodegradable polymers and natural preservatives is an emerging area of research in food industry. Zanthoxylum rhetsa is low cost spice and is rich in bioactive compounds. This can be used as the active component in biodegradable films that can extend the shelf life of the meat packed in it at chilled temperatures. Thus the burden on energy as well as petroleum resources can be minimized.

Research – Staphylococcus aureus gene involved in virulence, biofilm formation and resistance to certain antibiotics: Study

Outbreak News Today Staph

An Institut Pasteur-CNRS research team has characterized a Staphylococcus aureus gene involved in virulence, biofilm formation and resistance to certain antibiotics. These results open up new avenues for understanding the control of S. aureus virulence mechanisms. This work was recently published in the journal PLoS Pathogens.

Staphylococcus aureus is part of the natural skin flora, preferentially colonizing external mucosa in 30 to 50% of the population, healthy carriers who develop no symptoms. But it is also a major human pathogen, causing diseases ranging from skin lesions (boils, impetigo, etc.) to endocarditis, acute pneumonia, osteomyelitis or sepsis. It is the leading Gram-positive bacterium responsible for nosocomial infections (hospital acquired infections). The most dangerous strains are those that display resistance to multiple antibiotics. This is the case of MRSA, resistant to Methicillin, widespread in hospitals and posing a major public health concern.

Research – Staphylococcus aureus: A new mechanism involved in virulence and antibiotic resistance

Science Daily 

 

An Institut Pasteur-CNRS research team has characterized a Staphylococcus aureus gene involved in virulence, biofilm formation and resistance to certain antibiotics. These results open up new avenues for understanding the control of S. aureus virulence mechanisms. This work was recently published in the journal PLoS Pathogens.

Staphylococcus aureus is part of the natural skin flora, preferentially colonizing external mucosa in 30 to 50% of the population, healthy carriers who develop no symptoms. But it is also a major human pathogen, causing diseases ranging from skin lesions (boils, impetigo, etc.) to endocarditis, acute pneumonia, osteomyelitis or sepsis. It is the leading Gram-positive bacterium responsible for nosocomial infections (hospital acquired infections). The most dangerous strains are those that display resistance to multiple antibiotics. This is the case of MRSA[1], resistant to Meticillin, widespread in hospitals and posing a major public health concern.

Europe – European surveillance shows high levels of drug resistance in zoonotic bacteria

CIDRAP

A surveillance report today from European health and food safety agencies indicates that antibiotic resistance in zoonotic bacteria from humans, food, and animals on the continent remains at high levels, with notable levels of multidrug resistance in two common causes of foodborne illness in humans.

The report is based on 2016 data provided by 28 EU member states and jointly analyzed by the European Centre for Disease Prevention and Control (ECDC) and the European Food Safety Authority (EFSA). It addresses resistance in bacterial isolates of zoonotic Salmonella and Campylobacter from humans, food, and poultry, along with resistance levels and mechanisms in indicator Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) in animals and food.

Zoonotic bacteria are organisms that are transmissible between animals and humans, either through direct exposure or through consumption of contaminated meat. The ECDC and EFSA have been collecting and analyzing data submitted by EU countries on these bacteria to monitor for levels of antibiotic resistance since 2013.

RASFF Alerts – Listeria monocytogenes – Raw Milk Cheese – Tuna Salad- E.coli – Razor Clams – Beef – Staphylococcus – Raw Ham

Food Testing - Eurofins

RASFF -Listeria monocytogenes (510 CFU/g) in raw milk cheese from Italy in Austria

RASFF – Listeria monocytogenes in chilled spicy tuna salad from Belgium

RASFF -shigatoxin-producing Escherichia coli in boneless beef meat (Bos taurus) from Brazil in the Netherlands

RASFF -too high count of Escherichia coli (490 MPN/100g) in live razor clams from Ireland

RASFF -coagulase-positive Staphylococcus (400 000 /g) in raw ham from Spain in France

Research – Levels of microbial contamination of domestic refrigerators in Italy

Science Direct iStock_000012710183Small

Aim

According to the EFSA Report 2013, 32.7% of outbreaks of foodborne illness registered in Europe occurs within the home, due to inadequate hygienic behaviour of consumers when preparing foods in the kitchen. The efficacy of proper cleaning of cutting boards, dishes and cutlery in limiting microbial cross-contaminations in the kitchen has been documented many times, whereas few researches have been performed to determine the microbial load of the internal walls of domestic refrigerators, in Italy. The aim of this investigation is to ascertain the role played by internal surfaces of home refrigerators as possible sources of microbial contamination of foods.

Material and methods

We analyzed 293 domestic refrigerators of students or workers at the university campus of Agripolis (Legnaro, Italy). For each refrigerator, 2 internal surfaces were sampled using sponge-bags. The amounts of total viable count (TVC), Gram-negative spoiling bacteria, moulds and yeasts and the main pathogenic bacterial species were determined.

Results

TVCs greater than 1 log CFU cm−2 are in a little over 50% of the samples analyzed and are found mainly on the bottom of the refrigerator (61%) compared to the walls (39%) (P < 0.001). Even for other microbial counts the risk ratio of finding them on the bottom of the refrigerator is significantly higher than on the walls; the possibility of there being a finding on the bottom with respect to the walls varies from 2.5 to 8.5 times respectively for moulds and Aeromonas spp. Salmonella spp. was found in 1.7% of the samples, Bacillus cereus in 5.6%, Coagulase-positive staphylococci (CPS) in 4%, the prevalence of which is always higher on the bottom of the refrigerator. Listeria monocytogenes and Yersinia enterocolitica were never found.

Conclusions

It is necessary to better educate consumers to clean their appliances more frequently.

USA – USDA Recall – Staphylococcus Enterotoxin – Pork Sausages

USDAEurofins Food Testing UK

Lee Bros. Foodservice Inc., a San Jose, Calif., establishment, is recalling 740 pounds of sausage products that may be contaminated with Staphylococcus aureus enterotoxin, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced today.

The following products are subject to recall: [View Label]

  • 16 oz packages of Lee’s Sandwiches brand Pork Sausages produced on 2/11/13 with an identifying code “042P” printed on the back of the package
  • 16 oz packages of Lee’s Sandwiches brand Pork and Chicken Sausages produced on 2/12/13 with an identifying code of “043PC” printed on the back of the package

Each package bears the establishment number “Est. 11041” inside the USDA Mark of Inspection. The products were sold at the wholesale and retail level in Arizona, California, Oklahoma, Nevada, Texas and online.

The problem was discovered by FSIS personnel during a food safety assessment. The inspector was reviewing processing records and found that the water level in the product may have been high enough to allow for the production of Staphylococcus aureus enterotoxin. FSIS has received no reports of illnesses associated with consumption of these products. Individuals concerned about an illness should contact a physician.

Research – MRSA Treatment

News NortheasternStaphylococcus

Recent work from Uni­ver­sity Dis­tin­guished Pro­fessor of Biology Kim Lewis promises to over­come one of the leading public health threats of our time. In a ground­breaking study pub­lished Wednesday in the journal Nature, Lewis’ team presents a novel approach to treat and elim­i­nate methi­cillin resis­tant staphy­lo­coccus aureus, or MRSA, a potent bac­terium whose resis­tance to antibi­otics has kept it one step ahead of researchers. That is, until now.

Bac­teria such as MRSA have evolved to actively resist cer­tain antibi­otics, a fact that has gen­er­ated sig­nif­i­cant interest among the sci­en­tific and med­ical com­mu­ni­ties. But Lewis, Director of Northeastern’s Antimi­cro­bial Dis­covery Center, sus­pected that a dif­ferent adap­tive func­tion of bac­teria might be the true cul­prit in making these infec­tions so devastating. –

Research – Effect of Pickling on Pathogens in Hard Boiled Eggs

Journal of Food BiochemistryEGGS

Traditional methods of food preservation, such as pickling, have reemerged as popular approaches for home and commercial food processing. The process of pickling hard-cooked eggs (HCE) can be an effective method of preventing the growth and survival of pathogenic microorganisms. Because of the variety of pickling processes, HCE manufacturers must validate their pickling process for Food and Drug Administration (FDA) approval and demonstrate 5-log reductions of pathogens associated with the product. In this study, a rapid one-step pickling process was evaluated for its ability to reduce Salmonella spp., Listeria monocytogenes, Staphylococcus aureus and Escherichia coli O157:H7 inoculated onto HCE for 14 days at room temperature. All pathogens inoculated onto HCE were reduced by (>5.0 logs) within 24 h and were undetectable following enrichment. The data obtained in this study may be of interest to regulatory officials and processors of HCE seeking pathogen reduction validation for their products.

RASFF Alerts – Histamine – Canned Tuna – Listeria monocytogenes – Raw Milk Cheese – Staph Toxin in Tuna

RASFF -Histamine (1524 mg/kg – ppm) in cannned tuna in olive oil from Spain in Italy

RASFF -Listeria monocytogenes (100 CFU/g) in chilled raw milk cheese from France

RASFF – Staphylococcal enterotoxin (presence /25g) in chilled tuna from Suriname, via the Netherlands in Sweden