Tag Archives: staphylococcus aureus

Research – 1,000 Year Old Recipe for MRSA Cure – Staphylococcus aureus

StaphBBC

Scientists have recreated a 9th Century Anglo-Saxon remedy using onion, garlic and part of a cow’s stomach.

They were “astonished” to find the 1,000 year old treatment almost completely wiped out staphylococcus aureus, otherwise known as MRSA.

Their findings will be presented at a national microbiology conference.

Australia – FSANZ – Lamingtons Recall – Staphylococcus aureus

FSANZ Staph

Metcash Food & Grocery Ltd has recalled Baker’s Oven Choc Lamingtons 4 Pack from IGA and Independent stores in QLD, Northern NSW and WA due to Staphylococcus aureus contamination. Food products contaminated with Staphylococcus aureus may cause illness if consumed. Any consumers concerned about their health should seek medical advice and should return the product to the place of purchase for a full refund.

 

Research – Soaps Promote The Colonisation Of Staphylococcus Aureus Bacteria In Human Noses

HACCP Europa Staphylococcus

An antimicrobial agent found in common household soaps, shampoos and toothpastes may be finding its way inside human noses where it promotes the colonization of Staphylococcus aureus bacteria and could predispose some people to infection. Researchers at the University of Michigan report their findings this week in a study published in mBio®, the online open-access journal of the American Society for Microbiology.

USA – USDA Recall – Staphylococcus Enterotoxin – Pork Sausages

USDAEurofins Food Testing UK

Lee Bros. Foodservice Inc., a San Jose, Calif., establishment, is recalling 740 pounds of sausage products that may be contaminated with Staphylococcus aureus enterotoxin, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced today.

The following products are subject to recall: [View Label]

  • 16 oz packages of Lee’s Sandwiches brand Pork Sausages produced on 2/11/13 with an identifying code “042P” printed on the back of the package
  • 16 oz packages of Lee’s Sandwiches brand Pork and Chicken Sausages produced on 2/12/13 with an identifying code of “043PC” printed on the back of the package

Each package bears the establishment number “Est. 11041” inside the USDA Mark of Inspection. The products were sold at the wholesale and retail level in Arizona, California, Oklahoma, Nevada, Texas and online.

The problem was discovered by FSIS personnel during a food safety assessment. The inspector was reviewing processing records and found that the water level in the product may have been high enough to allow for the production of Staphylococcus aureus enterotoxin. FSIS has received no reports of illnesses associated with consumption of these products. Individuals concerned about an illness should contact a physician.

Research – High-Pressure Processing and Boiling Water Treatments for Reducing Listeria monocytogenes, Escherichia coli O157:H7, Salmonella spp., and Staphylococcus aureus During Beef Jerky Processing

Science Direct

Beef jerky is a convenient, ready-to-eat meat product, but requires processing lethality steps to ensure the safety of the product. Previous outbreaks involving various jerky products have highlighted the risks associated with jerky and the importance of utilizing pathogen interventions during processing. In this study, two alternative interventions were evaluated for reducing pathogen populations during jerky processing. Results demonstrated that high pressure processing (HPP; two treatments of 550 MPa, 60 s) could produce significant (p < 0.05), but variable reductions (6.83 and 4.45 log10 CFU/strip) of Salmonella spp. and Escherichia coli O157:H7, respectively, on resulting beef jerky. HPP treatments, however, produced minor reductions (p < 0.05) of Gram-positive pathogens, resulting in reductions of 1.28 and 1.32 log10 CFU/strip of Listeria monocytogenes and Staphylococcus aureus, respectively. Alternatively, boiling water (100 ± 2 °C) treatments (20–30 s) used after marination and prior to dehydration, reduced Salmonella spp., E. coli O157:H7, L. monocytogenes, and S. aureus populations >5.0 log10 CFU/strip in resulting beef jerky. Thus, 20 or 30 s boiling water (100 ± 2 °C) treatments could be effective interventions for commercial jerky processors or home food preservers. Future validation of these processes in-plant could provide processors and regulators with alternative strategies for safe and shelf-stable jerky products.

Research – Food Poisoning and Online Food Shopping

Liebert Open Access

Food sold over the internet is an emerging business that also presents a concern with regard to food safety. A nationwide foodborne disease outbreak associated with sandwiches purchased from an online shop in July 2010 is reported. Consumers were telephone interviewed with a structured questionnaire and specimens were collected for etiological examination. A total of 886 consumers were successfully contacted and completed the questionnaires; 36.6% had become ill, with a median incubation period of 18 h (range, 6–66 h). The major symptoms included diarrhea (89.2%), abdominal pain (69.8%), fever (47.5%), headache (32.7%), and vomiting (17.3%). Microbiological laboratories isolated Salmonella enterica serovar Enteritidis, Salmonella Virchow, Staphylococcus aureus, Bacillus cereus, and enterotoxigenic Escherichia coli from the contaminated sandwiches, Salmonella Enteritidis and Salmonella Virchow from the patients, and Salmonella Enteritidis and Staphylococcus aureus from food handlers. Pulsed-field gel electrophoresis genotyping suggested a common origin of Salmonella bacteria recovered from the patients, food, and a food handler. Among the pathogens detected, the symptoms and incubation period indicated that Salmonella, likely of egg origin, was the probable causative agent of the outbreak. This outbreak illustrates the importance of meticulous hygiene practices during food preparation and temperature control during food shipment and the food safety challenges posed by online food–shopping services.

UK – Research – Livestock Associated with MSRA

Food Safety NewsStaphylococcus

The U.K. Department of Health (DH) has identified the presence of livestock-associated MRSA in turkeys and chickens on a farm in East Anglia. (MRSA stands for methicillin-resistant Staphylococcus aureus.)

The BBC reports that it’s the first case of LA-MRSA in poultry in the U.K., and that two-thirds of the turkeys on the unnamed farm were infected. Hundreds of turkeys may have already been sold to local retail outlets.

GOV.UK

The Animal Health and Veterinary Laboratories Agency (AHVLA) has identified the presence of Livestock-Associated Meticillin Resistant Staphylococcus aureus (LA-MRSA) in poultry on a farm in East Anglia.

LA-MRSA is not the same as those that cause the healthcare associated infections that affect people. The risk of getting LA-MRSA from eating poultry meat is very low if the meat is handled hygienically and cooked thoroughly to kill any bacteria. The risk of the general public catching LA-MRSA from an animal is also very low.

Research – Antibiotic-Free Turkey Less Likely to Harbor Resistant Bacteria

Food Safety News

Ground turkey from birds raised without antibiotics is less likely to be contaminated with antibiotic-resistant bacteria than conventional ground turkey, according to a new study published by Consumer Reports today.

The group tested 257 samples of raw ground turkey meat and patties, purchased from major retailers nationwide, for Enterococcus, E. coli, Staphylococcus aureus, Salmonella, and Campylobacter and then looked at what portion of these bacteria were resistant to antibiotics. They found high levels of bacteria overall – 90 percent of samples tested positive for one of the five – and more than half were resistant to three or more classes of antibiotics. But the bugs found in products labeled “no antibiotics,” “organic,” or “raised without antibiotics” were resistant to fewer antibiotics than their conventional counterparts.

Research – Staphylococcus Nasal Colonisation

HACCPEUROPAStaphylococcus

Staphylococcus aureus (S. aureus) is one of the five most common causes of infections after injury or surgery and can be a food contaminant. Around one third of healthy individuals carry this bacteria in their noses, pharynx and on their skin. In normal healthy and immunocompentent person, S. aureus colonization of the skin, intestinal tract, or nasopharynx does not lead to any symptoms or disease.

Nasal colonisation by Staphylococcus aureus depends upon clumping factor B binding to the squamous epithelial cell envelope protein loricrin.

Research Articles – Pulsed Electronic Field Milk – PCR Method Vibrio – Listeria Detection Culture Methods

Science Direct

Abstract

Lethal and sublethal injury of two Gram-positive (Staphylococcus aureus and Listeria monocytogenes) and one Gram-negative (Escherichia coli) bacteria in milk by pulsed electric fields (PEF) were determined using non-selective and selective media. PEF inactivation kinetics including lethal and sublethal injury fractions was also studied. The proportion of the sublethally injured microbial cells depended on the microorganisms, electric field strength and treatment time. The proportion of sublethally injured microbial cells reached maximum after a specific PEF treatment, and it kept constant or progressively decreased at greater electric field strengths and with longer PEF treatments. For the strain of L. monocytogenes, the proportion of sublethally injured cells increased from 18.98% to 43.64% with the increasing electric field strength from15 to 30 kV/cm. While for the strains of E. coli and S. aureus, the proportion of sublethally injured cells achieved the maximum (40.74% and 36.51%, respectively) at 25 kV/cm and then decreased. The proportion of the sublethally injured microbial cells reached maximum at 400 μs (S. aureus and L. monocytogenes) or 500 μs (E. coli), and decreased at longer treatments at 30 kV/cm. The PEF inactivation kinetics including lethal and sublethally injured fractions was analyzed by the Hülsheger model, and the model parameters (EC, tC, kE, bt) for lethal and sublethal injury were also calculated.

Science Direct

Abstract

A previously developed multiplex PCR targeting gyrB of Vibrios at genus level and pntA genes for specific detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus was evaluated. The sensitivity of the multiplex PCR on spiked seafood was 1.5 × 103 CFU g−1. One hundred and fifty seafood samples were collected from retail stores and hypermarkets in different locations in Kuala Lumpur, Petaling Jaya and Seri Kembangan. The prevalence of V. parahaemolyticus was 29% (43/150). The pntA primers for V. parahaemolyticus detection were 100% specific and comparable to the toxR gene-based PCR. Six (12%) and 2 (4%) isolates contained trh and tdh genes, respectively. Repetitive Extragenic Palindromic PCR (REP-PCR) was used to genetically characterize the V. parahaemolyticus isolates in which 41 REP profiles were observed and all the isolates were categorized into 11 distinct clusters at the similarity of 80%. tdh-positive isolates shared a low level of similarity with trh-positive isolates. The prevalence of V. parahaemolyticus and particularly the presence of virulent gene such as trh and tdh among the isolates reiterate a high risk of contamination for seafood consumers in Malaysia. DNA fingerprinting of V. parahaemolyticus in this study indicates a high genetic diversity among the isolates and REP-PCR was able to distinguish the isolates with different virulotypes.

Science Direct

Abstract

The objectives of this study were to determine the prevalence of Listeria spp., specifically Listeria monocytogenes in ready-to-eat (RTE) foods and ascertain the efficiency of detecting L. monocytogenes with different selective culture media. A total of 396 RTE food samples were purchased from hypermarkets and streetside hawker stalls to examine the presence of Listeria spp. and L. monocytogenes. The presumptive isolates were characterized biochemically and were further confirmed by Polymerase Chain Reaction (PCR). Out of 396 samples, Listeria spp. was detected in 71 (17.9%) samples in which 45 (11.4%) were positive for L. monocytogenes. Among the studied RTE foods, salads and vegetables had the highest prevalence (14.7%) of L. monocytogenes, followed by chicken and chicken products (13.2%), beverages (10%), eggs and egg products (9.5%), beef and beef products (6.7%), lunch boxes (6.7%) and seafood and seafood products (6.7%). Both Listeria selective agar and PALCAM agar displayed a low sensitivity and specificity in L. monocytogenes detection compared to CHROMagar™ Listeria which demonstrated 96.9% of sensitivity and 99.1% of specificity in L. monocytogenes detection in naturally-contaminated foods. In conclusion, this work revealed consumption of RTE foods as a potential risk of listeriosis in this region. The high contamination rate of L. monocytogenes in salads and vegetables from hypermarkets and streetside hawker stalls was of great concern due to emerging fresh produce-borne L.monocytogenes globally. The scenario warrants further surveillance and action by the local authority to control the incidence of L. monocytogenes contamination in RTE foods.