Category Archives: Microbiology

Norway – Outbreak of Salmonella infection

Matportalen

The Norwegian Food Safety Authority has been notified by the Norwegian Institute of Public Health about a national outbreak of the gastrointestinal bacterium Salmonella Napoli. So far, infection has been detected in seven people. The source of infection is not known.

An outbreak investigation has been initiated in collaboration between the Norwegian Food Safety Authority, the Norwegian Institute of Public Health, the Veterinary Institute and municipal chief medical officers. It is currently unknown what the source of infection is, but it is common for gastrointestinal bacteria to become infected through food. According to the Norwegian Institute of Public Health, the most common symptoms are diarrhoea, headache, abdominal pain, nausea and possibly fever.

– The Norwegian Food Safety Authority collects information from the sick persons about what they have eaten and what they have been in contact with. It may also be relevant to take samples of food and food packaging, says Anne Mæland, senior advisor in the biological food safety section of the Norwegian Food Safety Authority.

Outbreak investigation takes time and the work is complicated. It is not always possible to find the source of infection in such outbreaks. If a common source of infection from food, animals or the environment is identified, the Norwegian Food Safety Authority will follow up the tracing work along the food chain.

Research – Pathogen that plagues food processing plants eradicated by blue light – Listeria monocytogenes

News Wise

Newswise — Washington, D.C. – Blue light kills both dried cells and biofilms of the pathogen Listeria monocytogenes, a frequent contaminant of food processing facilities. Demise of L. monocytogenes occurred quickest when cells or biofilms were placed on polystyrene, a widely used, transparent form of plastic. The research is published in Applied and Environmental Microbiology, a journal of the American Society for Microbiology.

“These results contribute to advancing our understanding of the potential of blue light to treat inert surfaces contaminated with L. monocytogenes,” said corresponding author Francisco Diez-Gonzalez, Ph.D., Director and Professor, Center for Food Safety, University of Georgia. Although biofilms of pathogens are generally powerfully resistant to being exterminated, the results suggest that blue light could effectively destroy L. monocytogenes.

In the study, the investigators deposited liquid suspensions of mixtures of 5 strains of L. monocytogenes on small, sterile rectangular plates made of 6 different materials, including polystyrene, stainless steel and silicone rubber, which were then allowed to dry. The investigators also used similar plates to grow biofilms, which they also allowed to dry.

Then, they shined blue light onto the biofilms and onto the dried suspensions of cells on the plates to determine the most effective combinations of doses and wavelengths, as well as the most effective surfaces on which to extirpate the pathogens.

“The application of blue light for controlling microbial contamination has the potential to offer an additional technology that could complement existing methods for disinfecting surfaces in contact with foods,” said Diez-Gonzalez, noting that blue light has been used for disinfection in hospitals. As compared to ultraviolet light, blue light offers reduced risk for the user, he said.

A post-doc in Diez-Gonzalez’ laboratory, Fereidoun Forghani, Ph.D., kick-started the investigation when—searching for new ideas—he came across the use of blue light as a potential antimicrobial intervention to sanitize surfaces. Forghani built some blue light prototypes and produced the first preliminary results treating pure cultures of Listeria.

Philippines – Food poisoning

Mindanao Times

ELEVEN individuals, mostly kids, were rushed to the hospital on Monday evening after they were downed by food poisoning at Sitio Matigsalug, Barangay Marilog, Marilog District.

According to the report, the caretaker Evangeline Palao told investigators that their employer Geraldina Bocuya from Deca Homes Mintal brought spaghetti when she visited their place at noon on Monday.

But after a few hours, the patients complained of stomach ache and started vomiting.

However, Tuazon said they could not yet ascertain if the spaghetti was the cause of the food poisoning or the tubod (spring water).

Taiwan – Over 20 Taiwanese elementary students suffer food poisoning from school lunches

Taiwan News

TAIPEI (Taiwan News) — As of Saturday (Oct. 14) night, more than 20 elementary students in Keelung City were admitted to the hospital due to food poisoning from school food.

The students, who all attend Shen-Mei Elementary School in Xinyi District, began reporting symptoms of vomiting Friday (Oct. 13) night, with students being admitted to emergency rooms the following day, reported UDN. Investigators suspect that a spoiled milk product served on Friday is responsible for the food poisoning.

Samples of milk, yogurt, and other food items served at the school have been taken for testing to determine the precise origin of the foodborne illness.

India – 16 students hospitalised due to suspected food poisoning at BMC-run school

Hindustan Times

16 students of a civic-run school in Mumbai were hospitalized due to suspected food poisoning after eating a midday meal provided by Shantai Mahila Industrial Co-operative Society. The affected students are now in stable condition and the food supply operation has ceased.

D Gangadharan, joint commissioner (education), BMC along with Kankal and other officials, visited the hospital to inspect the students’ well-being. Gangadharan said, “We examined the school premises to assess the situation. A sample of the food supplied at the school and other materials that were used in the kitchen has been sent to the BMC laboratory in G/North for testing, and the analysis report is expected shortly.”

Research – Risk assessment of norovirus and hepatitis A virus in strawberries imported into China

Wiley Online

Abstract

Norovirus (NoV) and hepatitis A virus (HAV) pose a considerable health risk worldwide. In recent years, many cases of virus infection caused by virus-contaminated strawberries have occurred worldwide. This study applied a critical control point system to analyze the main hazards during the production and marketing of strawberries imported into China and explore the key control points in the whole process. To further evaluate the risks in the supply chain, the established quantitative microbial risk assessment (QMRA) was used to determine the probability that residents would be infected with viruses after consuming imported strawberries. It was found that the risk of virus contamination from imported strawberries was low, and the virus contamination mainly results from water resources and personnel. This research helps the regulatory authorities formulate strategies to ensure the long-term microbial safety of imported strawberries. In addition, the methods may prove useful in evaluating the risks of other agricultural produce.

Research – Pathogenic E. coli in the Food Chain across the Arab Countries: A Descriptive Review

MDPI

Abstract

Foodborne bacterial infections caused by pathogens are a widespread problem in the Middle East, leading to significant economic losses and negative impacts on public health. This review aims to offer insights into the recent literature regarding the occurrence of harmful E. coli bacteria in the food supply of Arab countries. Additionally, it aims to summarize existing information on health issues and the state of resistance to antibiotics. The reviewed evidence highlights a lack of a comprehensive understanding of the extent to which harmful E. coli genes are present in the food supply of Arab countries. Efforts to identify the source of harmful E. coli in the Arab world through molecular characterization are limited. The Gulf Cooperation Council (GCC) countries have conducted few surveys specifically targeting harmful E. coli in the food supply. Despite having qualitative data that indicate the presence or absence of harmful E. coli, there is a noticeable absence of quantitative data regarding the actual numbers of harmful E. coli in chicken meat supplies across all Arab countries. While reports about harmful E. coli in animal-derived foods are common, especially in North African Arab countries, the literature emphasized in this review underscores the ongoing challenge that harmful E. coli pose to food safety and public health in Arab countries.

Research – New publication about microbiological hazards in fresh fruits and vegetables

FAO

Fresh fruits and vegetables contaminated with foodborne pathogens (bacteria, viruses, protozoa, helminths, etc.) have resulted in numerous outbreaks of foodborne illness, made headlines and caused trade disruptions around the world. To better understand these hazards, and how to prevent and mitigate the risks, FAO and WHO convened a meeting of scientific experts to identify and evaluate commodity-specific interventions used to reduce the risk of microbiological hazards in fresh fruit and vegetable production – from primary production to post-harvest activities, transportation, point of sale and preparation for consumption. A new report is now available and provides guidance, taking into consideration the effectiveness, practicality and suitability of the various approaches used in fruit and vegetable safety.

Prevention and control of microbiological hazards in fresh fruits and vegetables – Part 4: Specific commodities, which is volume 44 in the FAO/WHO Microbiological Risk Assessment Series, is the report of the meeting convened by the Secretariat of the Joint FAO/WHO Expert Meetings on Microbiological Risk Assessment (JEMRA). It addresses four commodity groups:  leafy vegetables and herbs, berries and tropical fruits, melons and tree fruits, and seeded and root vegetables.

This assessment updates and expands previous JEMRA work on the safety of fruits and vegetables and supports work being conducted by the Codex Alimentarius Commission on the development of guidelines for the control of Shiga toxin-producing Escherichia coli (STEC) in leafy vegetables and in sprouts.

Download the report

Germany -Meat of wild game animals should become safer

BFR

European network aims to further reduce health risks

Game meat, including those of red deer, wild boar, or pheasant, is among the foods with the smallest ecological footprint. These animals grow up in the wild, feeding on what na-ture provides, which also means that they can be exposed to various environmental con-taminants. Furthermore, wild animals can serve as carriers of zoonotic pathogens. The Eu-ropean network ‘Safety in the Game Meat Chain’ that will be established over the next four years under the leadership of the German Federal Institute for Risk Assessment (BfR),and promotes the exchange of knowledge regarding the health risks associated with game meat obtained through hunting for consumers. ‘Our goal is to make this valuable animal-derived food as safe as possible both in Europe and worldwide,’ says BfR President Professor Dr.Dr. Andreas Hensel. ‘We aim to minimize both chemical and microbial risks as much as possi-ble.’ The growing network currently includes 29 countries, encompassing not only EU member states but also non-EU countries, including those in the Western Balkans, Turkey, the United Kingdom, the United States, New Zealand, and Australia.

Over a four-year period, the five working groups of the network will focus on hunting and processing, game meat trade networks and the supply chain, as well as various biological and chemical hazards. To accomplish this, research activities from national level will be compiled and evaluated. Stakeholder groups from academia, industry, governmental institutions, and final consumers will receive ongoing updates on the new findings.

A central aim of the network is direct collaboration with stakeholder groups to translate knowledge into actions along the entire production chain, “from forest to fork” . The focus lies not only on undesirable substances from the environment (environmental con-taminants), but also on preventing or reducing the input of heavy metals, especially lead, from hunting ammunition. The biological hazards range from parasites such as Trichinella larvae, which can be transmitted to humans through the consumption of game meat, all the way to bacterial zoonotic agents including Salmonella and verotoxigenic Escherichia coli (VTEC), as well as viruses like hepatitis E (HEV) in wild boars.

Risks of both chemical and microbial origin that may arise during processing and trade of game meat, potentially contaminating the end product, will also be assessed. The goal is to disseminate scientific knowledge gained through the network from various regions and to align long-term food safety standards across borders.

The network also aims to gather insights into different hunting and training practices as well as national legal regulations and standards on meat inspection and hygiene for game meat in each country. The safety standards for cross-border trade in wild products are intended to be enhanced and harmonized. Additionally, the network places emphasis on educating con-sumers about the risks associated with and safe handling of game meat. Ultimately, this can support the consumer’s confidence in game meat products.

‘Safety in the Game Meat Chain’ is founded by the European Cooperation in Science and Technology (COST).

Useful links

SafeGameMeat COST Action (CA22166):
https://www.cost.eu/actions/CA22166/

Further information from the BfR website on game meat (in German)

SALMONELLA MODULATES METABOLISM DURING GROWTH UNDER CONDITIONS THAT INDUCE EXPRESSION OF VIRULENCE GENES.

SBGR Web

Salmonella enterica serovar Typhimurium (S. Typhimurium) is a facultative pathogen that uses complex mechanisms to invade and proliferate within mammalian host cells. To investigate possible contributions of metabolic processes to virulence in S. Typhimurium grown under conditions known to induce expression of virulence genes, we used a metabolomics-driven systems biology approach coupled with genome-scale modeling. First, we identified distinct metabolite profiles associated with bacteria grown in either rich or virulence-inducing media and report the most comprehensive coverage of the S. Typhimurium metabolome to date. Second, we applied an omics-informed genome-scale modeling analysis of the functional consequences of adaptive alterations in S. Typhimurium metabolism during growth under our conditions. Modeling efforts highlighted a decreased cellular capability to both produce and utilize intracellular amino acids during stationary phase culture in virulence conditions, despite significant abundance increases for these molecules as observed by our metabolomics measurements. Furthermore, analyses of omics data in the context of the metabolic model indicated rewiring of the metabolic network to support pathways associated with virulence. For example, cellular concentrations of polyamines were perturbed, as well as the predicted capacity for secretion and uptake.