Category Archives: E.coli

USA – CDC Confirms Multistates E.coli O121 Outbreak

Posted on March 30, 2013 by | Leave a comment

Food Safety NewsEcoli Istock

The U.S. Department of Agriculture Food Safety and Inspection Service has posted a distribution list of where affected products were sent. That list includes Wal Mart stores nationwide, Winn-Dixie stores in Florida, and a variety of retailers in Michigan.

At least 24 people in 15 states have fallen ill with E. coli O121 in an outbreak traced back to Farm Rich brand frozen pizzas, quesadillas, philly cheese steaks and mozzarella bites, the U.S. Centers for Disease Control and Prevention has confirmed, following initial reports Thursday evening.

Seven people have been hospitalized in connection to the products, which were sold nationwide. One patient has developed hemolytic uremic syndrome.

CDC

  • A total of 24 persons infected with the outbreak strain of Shiga       toxin-producing Escherichia coli O121 (STEC O121) have been       reported from 15 states.
    • 78% of ill persons are 21 years of age or younger.
    • 33% of ill persons have been hospitalized. One ill        person developed hemolytic uremic syndrome (HUS), a type of kidney        failure, and no deaths have been reported.
  • CDC and state public health officials are interviewing ill persons to       obtain information regarding foods they might have eaten and other       exposures in the week before illness.
    • Information available to date indicates that        consumption of Farm Rich brand frozen food products is one likely source        of infection for the ill persons in this outbreak.
  • Testing conducted by the New York State Department of Health,       Wadsworth Center Laboratory, identified the outbreak strain of STEC O121       in an open package of Farm Rich brand frozen chicken quesadillas       from an ill person’s home.

 

Leave a comment

Posted in Bacteria, E.coli, E.coli O121, Eurofins Laboratories, Food Hygiene, Food Illness, Food Inspections, Food Micro Blog, Food Microbiology, Food Poisoning, Food Safety, Food Safety Alert, Food Testing, Foodborne Illness, Hygiene, Methods, Microbiology, outbreak, Pathogen

Tagged ,

USA – Chicken Quesadilla – E.coli O121

Posted on March 29, 2013 by | Leave a comment

Ecoli BlogEcoli Istock

Rich Products Corporation, a Buffalo, NY firm, is recalling approximately 196,222 pounds of frozen chicken quesadilla and various other heat treated, not fully cooked frozen mini meals and snack items because they may be contaminated with E. coli O121, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced today.

The U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) was notified of a multistate investigation of E. coli O121 illnesses on March 19, 2013. Food samples were collected from an ill individual in New York as part of this investigation, and tested by the New York State Department of Health Wadsworth Laboratory. At present, the cluster includes 24 cases in 15 states. A sample of a Farm Rich frozen chicken mini quesadilla product from a New York case tested positive for the outbreak strain of E. coli O121. Eight cases in Michigan, Mississippi, New York, Ohio, Pennsylvania, Texas, and Virginia report consuming Farm Rich products. FSIS is continuing to work with federal and state public health partners on this investigation, including the New York State Department of Health, New York State Department of Agriculture & Markets, Food and Drug Administration, and Centers for Disease Control and Prevention.

Leave a comment

Posted in Bacteria, E.coli, E.coli O121, Eurofins Laboratories, Food Illness, Food Micro Blog, Food Microbiology, Food Poisoning, Food Safety, Food Safety Alert, Food Testing, Foodborne Illness, Methods, Microbiology, Pathogen, Recall

Tagged ,

USA – Hawaii – E.coli O157 Outbreak

Posted on March 29, 2013 by | 1 comment

Food Poisoning Journal

Lisa Kubota of Hawaii News Now reports that investigators with the Hawaii Department of Health are looking into at least nine people have now become ill from E. coli O157:H7.  The confirmed cases consist of three adults and six children. All of them live on Oahu except for a Canadian visitor who spent time on Oahu, but was later diagnosed on the Big Island. Officials are having trouble pinpointing the source of the infections.

Oahu healthcare providers recently received a letter from health officials warning them to be on the lookout for E. coli O157:H7. Around the same time, Dr. James Ireland saw a 67-year-old patient with symptoms like severe diarrhea and abdominal cramps.  Ireland said the man is now recovering after being hospitalized. There have been a total of 11 cases so far this year, including two unrelated to the current cluster. There were 20 last year, 9 the previous year, and 29 in 2010.

According to the state, three of the children in this latest group developed a life-threatening complication called Hemolytic Uremic Syndrome.

1 Comment

Posted in Bacteria, E.coli, E.coli O157, Food Hygiene, Food Illness, Food Inspections, Food Micro Blog, Food Microbiology, Food Poisoning, Food Safety, Food Safety Alert, Food Testing, Foodborne Illness, Hygiene, Illness, Microbiology, outbreak, Pathogen

Tagged ,

Research – Cranberry Antimicrobial – Multiple PCR for Pathogens

Posted on March 27, 2013 by | Leave a comment

Science Direct

The antimicrobial properties of the American cranberry were studied against Escherichia coli O157:H7, Listeria monocytogenes, and Lactobacillus rhamnosus to determine the effects on growth inhibition, membrane permeability, and injury. Cranberry powder was separated using a C-18 Sep-Pak cartridge into sugars plus organic acids (F1), monomeric phenolics (F2), and anthocyanins plus proanthocyanidins (F3). Fraction 3 was further separated into anthocyanins (F4) and proanthocyanidins (F5) using an LH-20 Sephadex column. Each fraction was diluted in the brain heart infusion (BHI) broth to determine the minimum inhibitory/bactericidal concentrations (MIC/MBC). L. monocytogenes was the most susceptible to cranberry fraction treatment with the lowest MIC/MBC for each treatment, followed by E. coli O157:H7 and L. rhamnosus. Membrane permeability and potential was studied using LIVE/DEAD viability assay and using Bis (1, 3-dibutylbarbituric acid) trimethine oxonol (DiBAC4), respectively. L. rhamnosus demonstrated the highest permeability followed by E. coli O157:H7, and L. monocytogenes. L. rhamnosus demonstrated the highest recovery followed by E. coli O157:H7, and L. monocytogenes. Each cranberry fraction demonstrated membrane hyperpolarization at their native pH, while F2, F3, and F5 demonstrated membrane depolarization at neutral pH. With this knowledge cranberry compounds may be used to prevent maladies and potentially substitute for synthetic preservatives and antibiotics.

Science Direct

We developed a rapid and reliable technique for simultaneous detection of Salmonella Typhimurium, Escherichia coli O157:H7 and Listeria monocytogenes that can be used in food products. Magnetic nano-beads (MNBs) based immunomagnetic separation (IMS) was used to separate the target bacterial cells while multiplex PCR (mPCR) was used to amplify the target genes. To detect only the viable bacteria, propidium monoazide (PMA) was applied to selectively suppress the DNA detection from dead cells. The results showed the detection limit of IMS-PMA-mPCR assay was about 102 CFU/ml (1.2 × 102 CFU/ml for S. Typhimurium, 4.0 × 102 CFU/ml for E. coli O157:H7 and 5.4 × 102 CFU/ml for L. monocytogenes) in pure culture and 103 CFU/g (5.1 × 103 CFU/g for S. Typhimurium, 7.5 × 103 CFU/g for E. coli O157:H7 and 8.4 × 103 CFU/g for L. monocytogenes) in spiking food products samples (lettuce, tomato and ground beef). This report has demonstrated for the first time, the effective use of rapid and reliable IMS combined with PMA treatment and mPCR assay for simultaneous detection of viable S. Typhimurium, E. coli O157:H7 and L. monocytogenes in spiked food samples. It is anticipated that the present approach will be applicable to simultaneous detection of the three target microorganisms for practical use.

 

Leave a comment

Posted in Bacteria, E.coli, E.coli O157, Food Hygiene, Food Illness, Food Micro Blog, Food Microbiology, Food Poisoning, Food Safety, Food Testing, Foodborne Illness, Listeria, Listeria monocytogenes, Microbiology, Pathogen, Salmonella

Tagged ,

USA Research – Microbiology of RTE Foods In University Canteens – Control of Pathogens on Fresh Cut Fruit

Posted on March 27, 2013 by | Leave a comment

Science Direct –

During a 10-year inspection survey (2001–2010), a microbiological study of ready-to-eat (RTE) foods and ready-to-bake frozen pastries from 15 canteens of the university campus was undertaken to determine their microbiological quality. The cumulative study revealed that the aerobic colony counts for the RTE product groups were as follows: from 106 to 108 CFU/g for 50% of sandwiches; under the detection limit (<10 CFU/g) for 88.6% of oven baked pastries; <105 CFU/g for 86.5% of desserts oven baked; from 103 to 109 CFU/g for desserts with dairy cream. The highest mean Enterobacteriaceae counts were recorded for desserts with dairy cream. The highest percentages of foodborne pathogens were: 20% Listeria monocytogenes and 12.5% Staphylococcus aureus in desserts with dairy cream; 17.5% Salmonella spp. and 8.5% presumptive Escherichia coli O157 in sandwiches; 14.6% Bacillus cereus in oven baked pastries. Aerobic colony counts were in the range 107–108 CFU/g for 48.8% of frozen pastries; whereas Enterobacteriaceae counts between 103 and 104 CFU/g were detected in 35.3%. Foodborne pathogens prevalences for frozen pastries were as follows: B. cereus, 31.8%; Salmonella spp., 28.6%; presumptive E. coli O157, 25%; S. aureus, 8.7%; L. monocytogenes, 8.7%. Improved sanitary conditions in the processing plants and precautionary measures are necessary for consumer protection.

Science Direct

The consumption of fresh-cut fruit has substantially risen over the last few years, leading to an increase in the number of outbreaks associated with fruit. Moreover, consumers are currently demanding wholesome, fresh-like, safe foods without added chemicals. As a response, the aim of this study was to determine if the naturally occurring microorganisms on fruit are “competitive with” or “antagonistic to” potentially encountered pathogens. Of the 97 and 107 isolates tested by co-inoculation with Escherichia coli O157:H7, Salmonella and Listeria innocua on fresh-cut apple and peach, respectively, and stored at 20 °C, seven showed a strong antagonistic capacity (more than 1-log unit reduction). One of the isolates, CPA-7, achieved the best reduction values (from 2.8 to 5.9-log units) and was the only isolate able to inhibit E. coli O157:H7 at refrigeration temperatures on both fruits. Therefore, CPA-7 was selected for further assays. Dose-response assays showed that CPA-7 should be present in at least the same amount as the pathogen to adequately reduce the numbers of the pathogen. From the results obtained in in vitro assays, competition seemed to be CPA-7’s mode of action against E. coli O157:H7. The CPA-7 strain was identified as Pseudomonas graminis. Thus, the results support the potential use of CPA-7 as a bioprotective agent against foodborne pathogens in minimally processed fruit.

Leave a comment

Posted in Bacillus, Bacillus cereus, Bacteria, E.coli, Food Hygiene, Food Micro Blog, Food Microbiology, Food Safety, Food Safety Alert, Food Testing, Foodborne Illness, Hygiene, Microbiology, Pathogen, Pseudomonas, Research, Salmonella

Tagged , ,

RASFF Alert – STec E.coli – Deer Meat

Posted on March 15, 2013 by | 1 comment

RASFF – Shigatoxin-producing Escherichia coli (VT1 positive) in frozen deer meat from Austria in Italy

1 Comment

Posted in E.coli, E.coli O103, E.coli O145, E.coli O157, EHEC, Eurofins Laboratories, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Poisoning, Food Safety, Food Safety Alert, Food Testing, Microbiology, Pathogen, RASFF, Recall, STEC

Tagged ,

UK -HPA -Burger Vans – Ecoli Contamination

Posted on March 14, 2013 by | Leave a comment

ITV News

HPA Report

Research from the Health Protection Agency (HPA) has revealed that food, water, chopping boards, cleaning cloths and security wristbands sampled from mobile and outdoor food vendors were contaminated with a range of bacteria including E.coli.

The bacteria, which originates from human or animal faeces is usually an indicator of either poor hygiene, undercooking or cross-contamination in the kitchen.

The events where samples were taken included 50 concerts or music festivals, 20 sports events, 39 carnivals, fetes and fairs and 44 ‘other’ events.

8% of food samples were of an unsatisfactory quality with a further 1% containing potentially hazardous levels of bacteria.

Water samples tested revealed that 27% contained unacceptable levels of coliform bacteria which can be found in the environment in soil, water and on plants and may also be a sign of faecal contamination.

E.coli and/or enterococci bacteria (of faecal origin) were found in 8%.

Leave a comment

Posted in Bacteria, E.coli, Food Hygiene, Food Illness, Food Inspections, Food Micro Blog, Food Microbiology, Food Poisoning, Food Safety, Food Testing, Foodborne Illness, Hand Washing, HPA, Hygiene, Microbiology, Pathogen, Research

Tagged , , , ,

Research – Antibiotic-Resistant Strain of E. Coli Increasing Among Older Adults and Residents of Nursing Homes

Posted on March 14, 2013 by | 1 comment

Science DirectEcoli Istock

Antibiotic-resistant Escherichia coli (E. coli) continues to proliferate, driven largely by expansion of a strain of E. coli know as sequence type ST131. A new study points to hospitals and long-term care facilities (LTCF) as settings in which this antibiotic-resistant strain is increasingly found.

The study is published in the April issue of Infection Control and Hospital Epidemiology, the journal of the Society for Healthcare Epidemiology of America.

E. coli is the most common gram-negative pathogen, causing both gastrointestinal disease and extraintestinal infections such as pneumonia, meningitis, and bloodstream, urinary tract, abdominal, and wound infections. Strains of E. coli that are resistant to single or multiple classes of antibiotics are becoming more prevalent. E. coli ST131 is commonly associated with fluoroquinolone resistance.

“The expansion of E. coli strain ST131 is recognized as a pandemic, but has received comparatively little attention in the United States,” said Ritu Banerjee, lead investigator of the study. “Alarmingly, the pace of new antibiotic development has not kept up with the emergence of antibiotic-resistant E. coli, making development of strategies to halt further emergence and spread of these strains a public health priority.”

1 Comment

Posted in Antibiotic Resistance, Bacteria, E.coli, Food Illness, Food Micro Blog, Food Microbiology, Food Safety, Food Technology, Food Testing, Hygiene, Microbiology, Pathogen, Research

RASFF Alerts – E.coli – Raw Milk – Beef – Live Mussels

Posted on March 11, 2013 by | Leave a comment

RASFF – Shigatoxin-producing Escherichia coli (presence /25g) in raw milk camembert from France in Germany

RASFF – Too high count of Escherichia coli (1100 MPN/100g) in live mussels (Mytilus galloprovincialis) from Spain in Italy

RASFF – Shigatoxin-producing Escherichia coli in chilled boneless beef (Bos taurus) from Australia in Netherlands

RASFF – Shigatoxin-producing Escherichia coli in chilled bovine meat (Bos taurus) from Brazil in the Netherlands

 

Leave a comment

Posted in Bacteria, E.coli, EHEC, Food Hygiene, Food Micro Blog, Food Microbiology, Food Safety, Food Safety Alert, Food Testing, Microbiology, Pathogen, RASFF, Recall

Tagged , , ,

Australia – Yoghurt Recall – E.coli

Posted on March 9, 2013 by | Leave a comment

FSANZFSANZ

Shaw River Buffalo Cheese has recalled Shaw River Buffalo Yoghurt from some small gourmet retailers and health food stores in NSW, QLD and VIC due to microbial (E. coli) contamination. Foods contaminated with E. coli may cause illness if consumed. Customers should return these products to the place of purchase for a full refund.

Date notified to FSANZ

8 March 2013

Food type

Dairy

Product name

Shaw River Buffalo Yoghurt (natural yoghurt)

Package description & size

500g and 1.2kg white plastic tamper-proof tub

Date marking

Best before 14 APR 13

Country of origin

Australia

Reason for recall

Microbial contamination (E. coli)

Leave a comment

Posted in Bacteria, E.coli, Eurofins Laboratories, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Poisoning, Food Safety, Food Safety Alert, Food Testing, FSANZ, Microbiology, Pathogen, Recall

Tagged , , ,