Category Archives: Research

Research – Officials confirm planned closure of Quaker Oats plant behind recall of dozens of products – Salmonella

Food Safety News

The mayor of an Illinois city has confirmed that PepsiCo will close a Quaker Oats plant that was temporarily shuttered during a recall of granola products in December 2023.

Mayor Ricky Williams Jr. said the plant will close on June 8, according to a Facebook post by the city of Danville, IL. Production at the plant included more than 60 products recalled in December 2023 and January 2024 because of Salmonella contamination.

The company confirmed the closure news on April 3. The company is shifting production of products made at the plant to other facilities.

Research – Detection of Extended Spectrum ß-Lactamase-Producing Escherichia coli with Biofilm Formation from Chicken Meat in Istanbul

MDPI

Abstract

Antimicrobial resistance is one of the major public health problems worldwide. This study aimed to detect the presence of extended-spectrum β-lactamase-(ESBL-)producing Escherichia (E.coli in chicken meat in Istanbul, Türkiye. Raw chicken meat samples (n = 208) were collected from different sale points and analyzed for ESBL-producing E. coli. In total, 101 (48.5%) isolates were confirmed as E. coli by PCR, of which 80/101 (79.2%) demonstrated multiple antibiotic resistance. Resistance against amoxicillin-clavulanic acid was most frequent (87.1%). Eighteen isolates (17.8%) demonstrated phenotypical ESBL resistance, as assessed by the double disc synergy test (DDST). Isolates were tested for the presence of β-lactamase genes and mobilized colistin-resistant genes. The blaTEM group was most frequently detected (97.02%), followed by blaCTX m (45.5%), blaSHV (9.9%), and blaOXA-2 (0.9%). However, mcr genes and blaNDM, blaKPCblaVIM, and blaOXA-48 genes were not found in any isolate. E. coli strains were tested for biofilm formation in six different media [Nutrient broth, LB broth, Tryptone Soya broth (TSB), TSB containing 1% sucrose, TSB containing 0.6% yeast extract, and BHI]. Biofilm formation by E. coli isolates (44/101, 43.5%) was highest in TSB with 1% sucrose. It is worth noting that all biofilm-producing isolates were found to harbor the blaTEM-1 gene, which can indicate a high level of antibiotic resistance. This is the first report about ESBL-producing E. coli in poultry meat, the exposure of consumers in Istanbul metropolitan areas, and the ability of E. coli from this region to produce biofilms.

Research – Food scientists develop framework to improve food quality and still kill pathogens

Phys Org

Food processors often use heat for pasteurization or sterilization to make food products safe by killing pathogens like salmonella and listeria, but high temperatures can degrade food quality. To ensure food safety, the industry sometimes relies on overly stringent standards that unnecessarily reduce , said Jennifer Acuff, assistant professor of food microbiology and safety for the Arkansas Agricultural Experiment Station, the research arm of the University of Arkansas System Division of Agriculture.

Focusing on low-moisture food products like powdered milk, Acuff and her team performed a study seeking a method that guarantees food safety while retaining the most vitamins, minerals and flavor depending on the food.

“This  encompassed microbiology, engineering, and statistics to provide the food industry with what we believe will be a tool to improve safety without compromising quality of their dried ,” Acuff said.

The process is not limited to low-moisture foods and may extend to other foods and processes, Acuff added.

Using data from a study on a harmless “surrogate” microorganism and a  called “bootstrapping,” the researchers developed a framework to provide food processors options within U.S. Food and Drug Administration guidelines.

Research – Concentration of foodborne viruses eluted from fresh and frozen produce: Applicability of ultrafiltration

Science Direct

Abstract

Foodborne illnesses involving raw and minimally processed foods are often caused by human noroviruses (HuNoV) and hepatitis A virus (HAV). Since food is contaminated usually with small numbers of virions, these must be eluted from the food surface and then concentrated for detection. The objective of this study was to optimize an ultrafiltration (UF) concentration method for HAV and HuNoVs present on various fresh and frozen produce. The detection range of the optimized method and its applicability to different food matrices was compared to the reference method ISO 15216-1:2017. Strawberry, raspberry, blackberry, lettuce, and green onion (25 g) were contaminated with HAV, HuNoV GI.7 and HuNoV GII.4 and then recovered therefrom by elution. A commercial benchtop UF device was used for the concentration step. Viral RNA was extracted and detected by RT-qPCR. From fresh strawberries, recovery of HAV loaded at 104 genome copies per sample was 30 ± 13 %, elution time had no significant impact, and UF membrane with an 80–100 kDa cut-off in combination with Tris-glycine elution buffer at pH 9.5 was found optimal. At lower copy numbers on fresh strawberry, at least 1 log lower numbers of HuNoV were detectable by the UF method (103 vs 104 GII.4 copies/sample and 101 vs 103 GI.7 copies/sample), while HAV was detected at 101 genome copies/sample by both methods. Except on raspberry, the UF method was usually equivalent to the ISO method regardless of the virus tested. The UF method makes rapid viral concentration possible, while supporting the filtration of large volume of sample. With fewer steps and shorter analysis time than the ISO method, this method could be suitable for routine analysis of viruses throughout the food production and surveillance chain.

Research – Preliminary Investigation towards the Use of Infrared Technology for Raw Milk Treatment

MDPI

Abstract

Infrared (IR) technology offers a promising solution for reducing microbiological loads in various food types while preserving their quality traits, such as flavour. However, research on IR’s application in complex matrices is limited. Therefore, our preliminary study aimed to evaluate its effectiveness in sanitizing bovine raw milk. We assessed the bacterial count before and after IR treatment by comparing volatile organic compound profiles via headspace extraction and GC/MS analysis. Our findings showed that higher energy levels led to a greater bacterial reduction. IR85 was the most effective in reducing Coliforms and Enterobacteriaceae in non-homogenised samples, with a reduction ranging from −1.01 to >−2.99 and from −1.66 to −3.09 Log CFU/mL, respectively. IR60 and 70 showed no efficacy, while IR80 had intermediate but still satisfactory effect. IR85 notably affected volatile compounds, particularly increasing hexanal (from 0.08 to 4.21 ng g−1) and dimethyl sulphone (from 10.76 to 26.40 ng g−1), while IR80 better preserved the aroma profile. As a result, only IR80 was tested with homogenised raw milk, demonstrating significant bacterial reduction (from >2.39 to 3.06 Log CFU/mL for Coliforms and from 1.90 to >2.45 Log CFU/mL for Enterobacteriaceae) and maintaining the aroma profile quality.

USA – 2023 – Research – Multiple Salmonella Outbreaks linked to Boston Restaurant

Food Poison Journal

In the summer of 2023, an outbreak of Salmonella Enteritidis infections occurred in the Boston area. Public health officials from the Boston Public Health Commission and the Massachusetts Department of Public Health conducted investigations into what would become three rounds of illnesses associated with food prepared at Los Amigos Taqueria located at 366 Washington Street in Brighton, Massachusetts. A total of 126 ill patrons were identified. Sixteen people were hospitalized. No one died.

Research – Turkish study reveals high prevalence of Salmonella in poultry meat

Food Safety News

A study in Turkey has found nearly 40 percent of chicken products sampled contained Salmonella.

Researchers investigated prevalence, antibiotic resistance, and genomic diversity of Salmonella in raw poultry meat products across Turkey.

A total of 293 samples were collected, including chicken breast, skinned drumstick, skinned chicken chop, wing, chicken offal, and chicken patty.

UK Research – Campylobacter data 2013 to 2022

Gov UK

Main points for 2022

The 2022 report shows that:

  • the number of reported Campylobacter cases in England decreased from 55,642 cases in 2021 to 54,461 cases in 2022, a decrease of 1,181 cases
  • the region that reported the highest number of Campylobacter laboratory reports was the South East with 9,540 reports; however, the region with the highest rate per 100,000 population was the North East with 133.3 per 100,000.
  • overall, 54% of Campylobacter laboratory-confirmed cases in England were male
  • the age group with the highest number of laboratory reports was the 50 to 59 year old age category
  • consistent with previous years’ 5-year median, in 2022 Campylobacter reporting peaked in the month of June
  • species was only reported for 23% of laboratory reports, the majority of which were Campylobacter jejuni (20% of all cases)

Research – Novel process decontaminates raw pet food, increases safety

Pet Food Industry

Dr. Keith Warriner will explain the gas-phase hydroxyl-radical process that offers intervention against Salmonella and other pathogens during his education session next month at Petfood Forum.

In recent years, the raw pet food industry has faced a series of challenges, with outbreaks and recalls linked to Salmonella contamination. This has led to a negative perception and concerns over the safety of raw pet foods.

USA – Alabama Cyclospora Outbreak 2023

Food Poison Journal

In the May of 2023, a significant increase in Cyclospora cases associated with a Mexican-style restaurant in Limestone County, Alabama was investigated by the Alabama Department of Public Health. An outbreak investigation was initiated on June 7, 2023. A case control study was launched on June 12. Exposures were reported between May 20 and June 6, 2023.