Category Archives: Food Microbiology Research

Research – Scientists develop “rapid” Salmonella detector

New Food Magazine

A team of researchers have been working on a device that they claim can detect the presence of salmonella in food.

According to the team, the device is an “easy-to-use” colorimetric assay that is based on a novel nucleic acid probe, cleaved by an RNase enzyme specific to the salmonella species. The researchers have said that this specific enzymatic cleavage principle “made it possible to build a sensitive but simple and portable test system using colloidal gold”.

Aremenia – 16 children with food poisoning ate boiled eggs, yogurt soup, pea soup, pasta pilaf in Armenia kindergarten

NEWS AM

At 10:30pm Thursday, the Ararat provincial center of the Food Safety Inspection Body (FSIB) of Armenia received a verbal report from the National Center for Disease Control and Prevention that six children, aged 3 to 4 years old, attending a nursery kindergarten in Artashat city were transferred to the Artashat hospital, with signs of food poisoning, FSIB noted in a statement.

One child was taken to Muratsan hospital in Yerevan.

But as of 10:30am Friday, the number of kids from the aforesaid kindergarten who were hospitalized had reached 16, and two of them are in the infectious disease ward.

The children are in satisfactory condition.

Initial diagnosis was unverified food poisoning and vomiting.

Samples were taken from the food on Thursday’s menu (boiled eggs, yogurt soup, pea soup, pasta pilaf) of this kindergarten. The samples have been submitted for lab tests, and the respective results will be announced later.

India – 135 people hospitalised in Malappuram after showing symptoms of food poisoning

The Hindu

As many as 135 people who attended a wedding at Kalady near Tavanur in Malappuram district on Wednesday were hospitalised after they developed symptoms of food poisoning.

Many of them are children. They were admitted to different hospitals at Edappal and neighbouring places with diarrhea, vomiting, and fever. Health officials said none was critical.

While 69 people from Thuruvanam island near Maranchery, who accompanied the bride to the party at the groom’s house at Kalady, fell sick, 66 of those who suffered food poisoning were from Kalady panchayat.

Health officials examined the premises where food poisoning was suspected and said it could have been caused by the water used during the function.

USA – Missoula County Health Officials issue warning about raw milk following possible Coxiella burnetii exposure

Food Poison Journal

Following potential exposure to bacteria from unpasteurized milk sold at a farmers’ market in Missoula County, the Missoula City-County Health Department is warning residents of the dangers of consuming unpasteurized, or “raw,” milk.

Milk that was recently sold at a local farmers market came from a herd where two cows tested positive for Coxiella burnetii, which is the bacteria that causes Q fever. While one of those cows had not yet produced milk, the other produced about 10% of the farmer’s yield.

“We don’t know if the cow was shedding the bacteria at the time it was milked, or if that cow’s milk was sold at the farmers market,” said Environmental Health Director Shannon Therriault. “So, we can’t say for sure whether anyone was exposed. However, what we do know is that unpasteurized milk can contain harmful bacteria that can make you and your loved ones sick.”

Unpasteurized milk products have been linked to outbreaks of E. coli, campylobacter, salmonella, brucella, listeria and cryptosporidium. In the case of Q fever, symptoms can take two or three weeks to present following exposure. According to the Centers for Disease Control and Prevention, symptoms of Q fever include fever, chills, fatigue, headache, muscle aches, nausea, vomiting, diarrhea, chest pain, stomach pain, weight loss and a non-productive cough.

USA – Boston Restaurant, Los Amigos Taqueria, Linked to Salmonella Outbreak

Outbreak News Today

kswfoodworld salmonella

According to the local media, a local restaurant chain has been shut down by the local health department due to “a slew of health violations” and “unsanitary working conditions that appear to be linked to a Salmonella outbreak in the area.”

The health department recommends that customers of the “Los Amigos Taqueria Brighton location between May 12 and May 18 to monitor themselves for symptoms of Salmonella infection, including fever, diarrhea, and abdominal pain, and to contact a healthcare provider for an evaluation and testing if appropriate.”

Health agents’ report said that “multiple rodent droppings” were also found on pineapple cans, bags of pinto beans, single-use beverage holders, and storage tables.

Research – U.S. heads up Codex ciguatera efforts

Food Safety News

The United States is to lead work on a document covering the prevention or reduction of ciguatera poisoning.

The plan was given the go-ahead at a recent Codex Committee on Contaminants in Foods meeting.

An electronic working group, chaired by the U.S. and co-chaired by France, Spain, and Panama, will work on a proposed code of practice or guidelines for consideration at the next meeting of the committee in 2024.

In 2022, the committee established a working group chaired by the U.S. and co-chaired by the European Union to prepare a discussion paper on the topic.

Generally, codes of practice provide practical guidance or measures to reduce or prevent a hazard in food, while a guideline gives higher-level principles and approaches to addressing a particular issue.

Research – Whole-Genome Sequencing of Shiga Toxin-Producing Escherichia coli for Characterization and Outbreak Investigation

MDPI

Abstract

Shiga toxin-producing Escherichia coli (STEC) causes high frequencies of foodborne infections worldwide and has been linked to numerous outbreaks each year. Pulsed-field gel electrophoresis (PFGE) has been the gold standard for surveillance until the recent transition to whole-genome sequencing (WGS). To further understand the genetic diversity and relatedness of outbreak isolates, a retrospective analysis of 510 clinical STEC isolates was conducted. Among the 34 STEC serogroups represented, most (59.6%) belonged to the predominant six non-O157 serogroups. Core genome single nucleotide polymorphism (SNP) analysis differentiated clusters of isolates with similar PFGE patterns and multilocus sequence types (STs). One serogroup O26 outbreak strain and another non-typeable (NT) strain, for instance, were identical by PFGE and clustered together by MLST; however, both were distantly related in the SNP analysis. In contrast, six outbreak-associated serogroup O5 strains clustered with five ST-175 serogroup O5 isolates, which were not part of the same outbreak as determined by PFGE. The use of high-quality SNP analyses enhanced the discrimination of these O5 outbreak strains into a single cluster. In all, this study demonstrates how public health laboratories can more rapidly use WGS and phylogenetics to identify related strains during outbreak investigations while simultaneously uncovering important genetic attributes that can inform treatment practices.

Research – Natural Antimicrobials for Listeria monocytogenes in Ready-to-Eat Meats: Current Challenges and Future Prospects

MDPI

Abstract

Listeria monocytogenes, an intra-cellular, Gram-positive, pathogenic bacterium, is one of the leading agents of foodborne illnesses. The morbidity of human listeriosis is low, but it has a high mortality rate of approximately 20% to 30%. L. monocytogenes is a psychotropic organism, making it a significant threat to ready-to-eat (RTE) meat product food safety. Listeria contamination is associated with the food processing environment or post-cooking cross-contamination events. The potential use of antimicrobials in packaging can reduce foodborne disease risk and spoilage. Novel antimicrobials can be advantageous for limiting Listeria and improving the shelf life of RTE meat. This review will discuss the Listeria occurrence in RTE meat products and potential natural antimicrobial additives for controlling Listeria.

Research – Multistate Outbreak of Salmonella Thompson Infections Linked to Seafood Exposure — United States, 2021

CDC

In July 2021, the Colorado Department of Public Health and Environment (CDPHE) laboratory identified a cluster of five Salmonella enterica serotype Thompson isolates related to one another within one allele difference, using whole genome multilocus sequence typing (wgMLST). These five isolates, submitted to the public health laboratory as is routine process for confirmatory testing of Salmonella, were highly related to those identified in a 2020 multistate investigation, during which traceback was conducted for sushi-grade tuna and salmon; a common supplier was not identified. The 2021 investigation commenced on August 5, 2021, with five patients living in Colorado, and one each in Missouri, Washington, and Wisconsin. During August–December 2021, CDC, CDPHE, public health and regulatory officials in several states, and the Food and Drug Administration (FDA) conducted epidemiologic, environmental, and laboratory investigations of this multistate outbreak of Salmonella Thompson. Isolates were genetically related to one another and to 2020 isolates within zero to one allele difference. Implicated seafood products were traced to a single seafood distributor, in which the outbreak strain was identified through environmental sampling, and in which inspection identified inadequate sanitization and opportunities for cross-contamination of raw fish. The distributor issued a voluntary recall of 16 seafood items with high potential for contamination and completed remediation actions. This outbreak illustrated the importance of effective cleaning and sanitizing procedures and implementation of controls. When multiple products are recalled during an outbreak investigation, collaboration between public health agencies and implicated facilities can help provide food safety information to restaurants, retailers, and consumers, and to ensure disposal of all recalled products.

USA – Dave’s Sushi to reopen after sickening 50 with 2 dead – Morel Mushrooms suspect

Food Poison Journal

Gallatin City-County Health Department Rescinds Closure Order and Institutes New Health Officer Order with Corrective Actions Required Prior to Dave’s Sushi Reopening

Since being ordered to close on April 20, 2023, food samples – including salmon and morel mushrooms – from Dave’s Sushi have been collected and analyzed. To date, all samples have come back negative for a series of pathogens and toxins. A thorough investigation led by the Gallatin City-County Health Department, with support from the Montana Department of Public Health and Human Services (DPHHS), the Centers of Disease Control and Prevention (CDC), and the Food and Drug Administration (FDA), is still ongoing and it is known that this investigation may not be able to identify a specific pathogen/toxin as a source of the outbreak that impacted 50 people. Three individuals had severe outcomes, including hospitalizations, and the deaths of two individuals are being investigated after eating at the restaurant. The manner and cause of death for these two individuals will remain pending until autopsy and toxicology results are available.