Tag Archives: research

USA – CDC – Multistate Outbreak of E.coli O157:H7 – Spinach

CDCE.coli O157

A total of 28 persons infected with the outbreak strain of Shiga toxin-producing Escherichia coliO157:H7 (STEC O157:H7) have been reported from five states, with most cases in New York.

42% of ill persons have been hospitalized. Two ill persons have developed hemolytic uremic syndrome (HUS), a type of kidney failure, and no deaths have been reported.

The outbreak was initially in New York. More recently, more ill persons in other states have been reported, and the investigation has expanded.

Thermo Scientific – Applied Food Testing Information Including Microbiology

Thermo Scientific

Thermo Fisher Scientific provides the latest information on food quality and safety applications, case studies or validated methods.

Our food quality and safety technical library is your resource for application expertise as well as news regarding events, regulations or public safety concerns.

Research – British Food Journal – Deliberate Food Safety Violations in the Food Chain

Emerald

Abstract :

Purpose – Business malpractice in supply chains raises food safety risks for downstream buyers including consumers. This paper analyses the multiplicity of behavioural factors influencing producers’ motivation to intentionally violate food safety norms.

Design/methodology/approach – We review existing disciplinary approaches for the analysis of behavioural risks. Based on this review, an analytical framework is developed which provides a base for an interdisciplinary institutional analysis of behavioural risks in food chains.

Findings – The reviewed approaches on behavioural risk share the view that deviance is the result of multi-goal and (potentially) opportunistic decision-making of bounded rational individuals. The analytical framework presented in this paper integrates these approaches.

Research limitations/implications – The analytical framework provides a rough categorization of behavioural drivers. It neither details the context-dependent subcomponents which determine the utility outcome within each category nor the methods that should be used to analyse them.

Originality/value – A behavioural economic analysis based on the framework means opening up the black box of the regulatees’ action situation by incorporating the subjectively perceived material incentives in addition to immaterial motivations such as reputation effects, social norms and community pressure into the analysis. Based on an understanding of producers’ motivation, proper institutional solutions can be implemented to enhance producers’ compliance with food safety norms.

Free webcast with 3M and Professor Stephen Forsythe on the rapid detection of foodborne pathogens

3M’s Food Safety business has launched a fast, accurate and easy-to-use pathogen detection system.

The new 3M™ Molecular Detection System, based on an innovative combination of two technologies – isothermal DNA amplification and ATP bioluminescence detection – delivers highly sensitive results in real-time with positive results in as early as 15 minutes and negative results in 75 minutes.

With ready-made reagents and only one test protocol for all target pathogens, the system is easy to use, allows for multiple pathogen detection during each run and reduces the chance for human error or contamination. Individual assays for Salmonella, E. coli O157 (including H7) and Listeria spp are currently available.

For more details join a free webcast on Wednesday 27 June 2012 (3pm) presented by 3M and Professor Stephen Forsythe of Nottingham Trent University, follow this link to register.

2nd Link
http://view6.workcast.net/register?pak=4909146311582106&referrer=KW

Research – Shigella Genetics Tracked

Shigella Blog

Scientists have found new genetic information that shows how harmful bacteria cause the acute diarrheal disease shigellosis, which kills more than a million people worldwide each year.

The research, which could lead to the development of future treatments, was published today in the journal PLoS ONE. The study was led by Ohio University scientist Erin Murphy and doctoral student William Broach, with contributions from University of Nevada, Las Vegas and University of Texas at Austin researchers.

CDC Report on Illness from Mail Order Chicks and Ducks – Salmonella

CDC

A total of 93 persons infected with outbreak strains of Salmonella Infantis, Salmonella Newport, and Salmonella Lille have been reported from 23 states. There have been 18 hospitalisations from this outbreak.

Collaborative investigative efforts of local, state, and federal public health and agriculture officials linked this outbreak of human Salmonella infections to exposure to chicks and ducklings from a single mail-order hatchery in Ohio.

 

Research- Sheep E.coli O26 and Clostridium difficile

American Society of Microbiology

A previous national survey of Escherichia coli in Norwegian sheep detected eae-positive (eae+) E. coli O26:H11 isolates in 16.3% (80/491) of the flocks. The purpose of the present study was to evaluate the human-pathogenic potential of these ovine isolates by comparing them with E. coli O26 isolates from humans infected in Norway. All human E. coli O26 isolates studied carried the eae gene and shared flagellar type H11. Two-thirds of the sheep flocks and 95.1% of the patients harbored isolates containing arcA allele type 2 and espK and were classified as enterohemorrhagic E. coli (EHEC) (stx positive) or EHEC-like (stx negative). These isolates were further divided into group A (EspK2 positive), associated with stx2-EDL933 and stcEO103, and group B (EspK1 positive), associated with stx1a. Although the stx genes were more frequently present in isolates from patients (46.3%) than in those from sheep flocks (5%), more than half of the ovine isolates in the EHEC/EHEC-like group had multiple-locus variable number of tandem repeat analysis (MLVA) profiles that were identical to those seen in stx-positive human O26:H11 isolates. This indicates that EHEC-like ovine isolates may be able to acquire stx-carrying bacteriophages and thereby have the possibility to cause serious illness in humans. The remaining one-third of the sheep flocks and two of the patients had isolates fulfilling the criteria for atypical enteropathogenic E. coli (aEPEC): arcA allele type 1 and espK negative (group C). The majority of these ovine isolates showed MLVA profiles not previously seen in E. coli O26:H11 isolates from humans. However, according to their virulence gene profile, the aEPEC ovine isolates should be considered potentially pathogenic for humans. In conclusion, sheep are an important reservoir of human-pathogenic E. coli O26:H11 isolates in Norway.

American Society of Microbiology

The prevalence of Clostridium difficile in retail meat samples has varied widely. The food supply may be a source for C. difficile infections. A total of 102 ground meat and sausage samples from 3 grocers in Pittsburgh, PA, were cultured for C. difficile. Brand A pork sausages were resampled between May 2011 and January 2012. Two out of 102 (2.0%) meat products initially sampled were positive for C. difficile; both were pork sausage from brand A from the same processing facility (facility A). On subsequent sampling of brand A products, 10/19 samples from processing facility A and 1/10 samples from 3 other facilities were positive for C. difficile. The isolates recovered were inferred ribotype 078, comprising 6 genotypes. The prevalence of C. difficile in retail meat may not be as high as previously reported in North America. When contamination occurs, it may be related to events at processing facilities.

USDA Food Microbiology Guidelines for Laboratories

USDA 

The Guidebook contains current protocols for analytical tests required by FSIS regulatory activities on meat, poultry and egg products.1 Specifically, microbiological methods are presented for sample preparation, isolation and identification of the major foodborne pathogenic microorganisms and their toxins, meat tissue species identification, and the detection of extraneous materials and antimicrobial residues. Media and reagent formulations, and Most Probable Number Tables are contained in an appendix.

Research Netherlands – Norovirus Implications-Food

Cambridge Journals

Noroviruses are an important cause of acute gastroenteritis in humans. We incorporated new insights gained over the past decade in an updated estimate of the disease burden of (foodborne) norovirus illness in The Netherlands in 2009. The disease outcomes – non-consulting cases, visiting a general practitioner, hospitalization and mortality – and the foodborne proportion were derived from cohort studies, surveillance data and literature. Age-specific incidence estimates were applied to the population age distribution in The Netherlands in 2009. The general population incidence was 3800/100 000 (95% CI 2670–5460), including 0·4 fatal cases/100 000, resulting in 1622/100 000 (95% CI 966–2650) disability-adjusted life-years in a population of 16·5 million. The updated burden of norovirus is over twofold higher than previously estimated, due in particular to the new insights in case-fatality ratios. Results suggest that the burden of norovirus institutional outbreaks is relatively small compared to the burden of community-acquired norovirus infections.

E.coli HUS Syndrome

Food Poisoning Bulletin

These are 10 things you should know if your child has an E. coli infection or has been diagnosed with HUS:

  • When your child is diagnosed with E. coli and/or HUS, you should contact your local health department immediately because additional testing needs to be done to determine if your child’s case of E. coli-HUS is part of an outbreak of illnesses. This is important because you may be able to provide information to health officials that helps them find the source of the outbreak.
  • You should not throw away food you have that you suspect is the source of your child’s E. coli infection.  The food may need to be tested for the presence of E. colibacteria and may be the “smoking gun” that determines the source of the outbreak.
  • The food eaten immediately before onset of illness is generally not the cause of the illnessbecause the incubation period for E. coli is from 3 to 10 days.
  • Because of the long incubation period, finding the source of an outbreak of E. coli-HUS can be difficult.During that time, the contaminated food responsible for the illnesses may have been eaten or thrown out. Even if a specific food source is not found, the outbreak investigation may lead to a legally responsible party, such as a restaurant.
  • Quickly finding the source of an E. coli-HUS outbreak can help prevent additional illnesses.
  • The CDC recommends that antibiotics not be used for E. coli infectionsbecause these medications increase the risk of developing HUS. You should make sure your doctor is aware of this.
  • Medical research and better understanding of HUS by medical professionals has improved care for HUS patients, lowering the mortality rate. Even if your child is extremely ill, he or she will most likely survive.
  • HUS may result in major long-term complications, including chronic renal failure, neurologic dysfunction, and hypertension.
  • Even if your child appears to have recovered, he or she is at greater risk of developing kidney problems in the future, including renal failure, and may need a kidney transplant.

It should be noted that a closely related illness, thrombotic thrombocytopenic purpura (TTP), is also caused by infection with  Shiga-toxin producing E. coli (generally E. coli O157). In adults, the diagnosis for E. coli victims with kidney failure is often TTP-HUS and often involves neurological problems, including stroke, that result in permanent brain damage.