Category Archives: T2 HT2

Research – Mycotoxin control in cereals: safe guarding food

Safe Food Net

The aim of this research was to review (and where possible improve/refine
current rapid screening methods for the mycotoxin T-2 and its metabolites in
cereals for human consumption.
Researchers engaged with the relevant sector of the cereal processing industry in
Ireland and the UK, and the relevant primary production sector, to gain detailed,
valuable information.
The research conducted was designed to clarify deficits in T-2 toxin control in the
relevant processing and primary production sectors, evaluate the robustness of
current analytical detection and control methods therein, augment same if deemed
necessary, and implement workable T-2 toxin control solutions at sectoral level.
Objectives
The specific objectives of the research project were to:
• Conduct a literature review of the most up-to-date scientific and
regulatory data for T-2 toxin and its metabolites, the specific environmental conditions required for their production, and the impact of climate change on their occurrence and distribution.
• Undertake a thorough analysis of the currently available commercial rapid analytical techniques for the screening of T-2 and HT-2 toxins in cereals for direct human consumption.
• Undertake a survey of the relevant processing and primary production sectors to determine what testing they currently conduct for mycotoxins, to fully understand the shortfalls in relation to mycotoxin monitoring and control within the industry.
Conduct a survey of oats and barley (n≥100) using mass spectrometry to identify contamination levels of, not only the mycotoxins of interest, i.e., T-2 and HT-2, but also all other regulated mycotoxins including aflatoxins, deoxynivalenol, zearalenone, fumonisins and ochratoxin A.
• Perform a thorough evaluation of a selected number of rapid test kits to ascertain fitness for purpose and determine how to adapt the most robust methods for better performance. If required, undertake a training workshop on the use of the best performing rapid testing kits.
• Engage with stakeholders to discuss the implications of the findings of
the project and produce a series of conclusions and recommendations
for stakeholders on the island of Ireland.

Research- Determination of Mycotoxins in Plant-Based Meat Alternatives (PBMAs) and Ingredients after Microwave Cooking

MDPI

Abstract

In this study, we investigate the role of microwave cooking in reducing mycotoxin contamination in plant-based food matrices, with a focus on veggie burgers (purchased and home-made) and their ingredients (soybean, potatoes, zucchini, carrots). Two different conditions were studied (Max–Min) that were 800 W for 60 s and 800 W for 90 s, respectively. The degradation patterns of aflatoxins (AFB1, AFB2, AFG1, AFG2), fumonisins (FB1, FB2, FB3), trichothecenes (T2, HT2, ZEA), and ochratoxin A (OTA) were studied. The extraction procedures were conducted with the QuEChERS extraction, and the analyses were conducted with liquid chromatography–tandem mass spectrometry (LC-MS/MS). Principal component analysis (PCA) showed that degradation under microwave cooking varies considerably across different food matrices and cooking conditions. This study provides valuable insights into the degradation of mycotoxins during microwave cooking and underscores the need for more research in this area to ensure food safety.

Norway – Analyzes of nutrients, heavy metals and mycotoxins in cereal products 2021

Mattilsynet

The Institute of Marine Research (HI) has, on behalf of the Norwegian Food Safety Authority, performed analyzes of nutrients, heavy metals and mycotoxins in grain products. The purpose of the project “Analyzes of nutrients, heavy metals and mycotoxins in grain products” is to obtain nutrient values ​​for the Food Table and control the products for foreign substances.

What we investigated:
  • The analyzed products are; oatmeal, organic oatmeal, sifted wheat flour, spelled wheat flour, organic wheat flour, whole wheat flour, sifted rye flour, whole rye flour, finely ground barley flour and bran.
  • The producers: AXA, Kolonihagen, Møllerens and Regal.
  • A total of 45 aggregate samples. Each sample consisted of three different batches from each manufacturer.
Period: The analyzes were performed in the period November-December 2019 (sample 1), and in August-September 2020 (sample 2) and five grain products in February 2021 (sample 3).
What we were looking for: The project has provided analysis data for the content of nutrients and foreign substances in grain products from the grocery trade.

 The project has provided analytical values ​​for ash, protein, fat, fatty acids, sugars, starch, dietary fiber, β -carotene, thiamine (vitamin B1), riboflavin (vitamin B2), niacin (vitamin B3), pyridoxine (B6), folate, vitamin C , vitamin E, vitamin K1, vitamin K2, calcium, magnesium, sodium, potassium, phosphorus, iron, zinc, selenium, iodine, arsenic, cadmium, lead and mercury, and the mycotoxins deoxynivalenol (DON), zearalenone, T2 HT2 and enniatin for 40 grain products. The grain products in sample 3 (5 products) were only analyzed for fat, protein, ash, starch and dietary fiber.

What we found: The results show that the levels of the heavy metals in the cereal products analyzed are below the limit values ​​that apply to arsenic, lead, cadmium, mercury and mycotoxins.

The results show a higher content of dietary fiber in Norwegian grain products than before. This can be explained by the use of an analysis method (AOAC 2009.1) that includes several dietary fiber compounds.

The Norwegian Food Safety Authority will inform affected companies about the analysis project.

Who performed the assignment? Institute of Marine Research