British Standards Update(ISO/EN) 2012 
Withdrawn / Obsolete Standards:
BS 5763-13, 1998 : Methods for microbiological examination of food and animal feeding stuffs — Enumeration of Escherichia coli. Colony-count. – Withdrawn
BS 4285 – 5.2, 1989 : Microbiological examination for dairy purposes. Ancillary methods — Methylene blue reduction test for cream and ice cream. – Obsolete
Newly Published Standards:
BS EN ISO 6887-4 : 2003 AMD 1- 2011
Specific rules for the preparation of products other than milk and milk products, meat and meat products, and fish and fishery products.
See update document.
BS EN ISO 16140 : 2003 AMD 1 – 2011
Microbiology of food and animal feeding stuffs — Protocol for the validation of alternative methods.
BS EN ISO 22119 : 2011
Microbiology of food and animal feeding stuffs — Realtime polymerase chain reaction
(PCR) for the detection of foodborne pathogens — General requirements and definitions.
BS EN ISO 22118 : 2011
Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection and quantification of food-borne pathogens — Performance characteristics.
BS EN ISO 11133 – 2 : 2003 AMD 1 : 2011
Microbiology of food and animal feeding stuffs — Guidelines on preparation and production of culture media — Part 2: Practical guidelines on performance testing of culture media
See update document.
New Work Item Procedures
BS EN ISO NWIP 16649-3
Microbiology of food and animal feed — Enumeration of β-glucuronidase positive Escherichia coli — Part 3: Most probable number technique using 5-bromo-4-chloro-3-indolyl-β-D-glucuronide
Disclaimer modified to state that only Milk and Milk product has not been validated for this method.
ISO 7251 and 6887-5 added to normative references.
Section 4 Principle now covers both Detection ( added ) as well as Enumeration.
Section 5 Media performance testing now states WDCM Culture references.
Section 9 now includes detection procedure as well as enumeration procedure.
Section 10 now gives guidance on reporting of Detection results.
NWIP ISO/TR 6579-3
Microbiology of food and animal feed – Horizontal method for the detection, enumeration and serotyping of Salmonella – Part 3 Guidelines for serotyping of Salmonella spp.
NWIP ISO 4833 -2
Microbiology of food and animal feed – Horizontal method for the enumeration of Microorganisms – Part 2: Colony count at 30 degrees C by the surface plating technique (ISO/DIS 4833-2:2012)
NWIP ISO / PDTS
Microbiology of food and animal feed — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Detection of botulinum type A, B, E and F neurotoxin producing Clostridia.
NWIP ISO/TS 11290-1 Horizontal method for the detection and enumeration of Listeria monocytogenes and other Listeria species —Part 1 Detection method
Proposal to reduce Fraser Broth enrichment to 24hrs
Proposal to confirm only one colony at a time until correct identification is achieved.
Use of Haemolysis, VP, Rhamnose and Xylose only for L. mono identification. Optional Gram stain and CAMP test
NWIP ISO/TS 11290-2 Horizontal method for the detection and enumeration of Listeria monocytogenes and other Listeria species — Part 2 Enumeration method.
Proposal to remove resuscitation stage
Proposal to use 1 x 140mm ALOA or 3 x 90mm ALOA plates
Proposal to incubate at 37C for 24 ± 3 hrs instead of 18 – 24hrs ( repeat for further 24 hrs )
Proposal for countable range on 140 mm plates at 235 cfu.
Proposal to use any non selective agar for colony streaking for confirmation purposes
( Blood agar, Nutrient agar etc )
Proposal to remove gram stain from confirmation and optional Catalase test
Use of Haemolysis, VP, Rhamnose and Xylose only for L. mono identification.
Expression of results now cross referenced to ISO 7218
NWIP ISO 7218 Microbiology of food and animal feeding stuffs – General requirements and guidance for microbiological examinations – Amendment 1 (ISO 7218:2007/DAM :2011)
FoodWorld 