Category Archives: Food Technology

UK Research- Norovirus in Food Outlets to be Mapped

Medical Press norovirus-2(1)

The University of Liverpool is leading a £2 million Food Standards Agency (FSA) project to map the occurrence of norovirus in food premises and industry workers.

Norovirus outbreaks can rapidly affect of people. In 2012 a batch of frozen strawberries infected 11,000 people in Germany, but there are significant gaps in the authorities’ understanding of which strains cause and which foods are the most likely to harbour the bacteria.

Researchers will produce data that will help the FSA to develop plans to reduce the infection by collecting swabs from work surfaces at more than 200 pubs, restaurants and hotels in the North West and South East of England.

 

USA – FDA to Permit the Irradiation of Shellfish

FDA FDA

The U.S. Food and Drug Administration (FDA or we) is amending our current food additive regulations to allow the safe use of ionizing radiation on crustaceans (e.g., crab, shrimp, lobster, crayfish, and prawns) to control foodborne pathogens and extend the shelf life. Our action responds to a food additive petition submitted by the National Fisheries Institute. Our decision is based on a rigorous safety assessment that considered 1) potential toxicity, 2) the effect of irradiation on nutrients, and 3) potential microbiological risk that may result from treating crustaceans with ionizing radiation. The evaluation also considered previous evaluations of the safety of irradiation of other foods including poultry, meat, molluscan shellfish, iceberg lettuce, and fresh spinach. This rule covers raw, frozen, cooked, partially cooked, shelled, or dried crustaceans, or cooked, or ready-to-cook, crustaceans processed with spices or small amounts of other food ingredients.

At the maximum permitted dose of 6.0 kiloGray, this new use of ionizing radiation will reduce, but not entirely eliminate, the number of pathogenic (illness causing) microorganisms in or on crustaceans. The maximum dosage of irradiation approved is capable of reducing a number of pathogens that may be found in crustaceans, including Listeria, Vibrio, and E. coli. Irradiation is not a substitute for proper food-handling practices; therefore crustaceans treated with ionizing radiation must be stored, handled, and cooked in the same way as non-irradiated foods.

 

Research – Radio Waves Killing Salmonella in Raw Eggs

Food Safet News EGGS

Raw chicken eggs are in many commonly consumed foods – eggnog, hollandaise sauce and mayonnaise, to name a few – and since about one of every 20,000 chicken eggs in the U.S. runs a high risk of containing Salmonella bacteria, the only way to be sure these bacteria are killed is to hard-boil the eggs or pasteurize them via hot-water immersion.

However, doing either of those things changes the taste, texture and color of the eggs, qualities that some consumers find objectionable. Now, prototype research studies by scientists at the Agricultural Research Service (ARS) and Princeton University have found that raw eggs artificially infected with Salmonella bacteria can be zapped almost completely free of it by using radio waves, and that these waves, combined with a subsequent hot-water bath, can more quickly pasteurize eggs without objectionable after-effects.

USA – Chicken Livers – Campylobacter

Orgeon Food Safety AlertsCampylobacter_jejuni_01

Since December 2013, Oregon health officials have been looking into the source of Campylobacteriosis that has sickened five individuals in Oregon and Ohio. All cases report eating undercooked or raw chicken livers; most cases consumed chicken livers prepared as pâté. The cases in Ohio ate chicken liver pâté while visiting Oregon. The Oregon Health Authority is working with USDA and CDC.

This is the second reported multistate outbreak of campylobacteriosis associated with consumption of undercooked chicken liver in the United States.

Oregon Live

A high-end chicken product enjoyed in high-end restaurants in Portland is part of a small food poisoning outbreak in Oregon.

Health officials issued a public health alert Tuesday, saying six people who consumed undercooked or raw chicken livers were infected with campylobacter, a common foodborne pathogen. Three people live in Oregon and two others were visiting the state from Ohio. The outbreak includes one person in Washington state who got sick after consuming chicken liver pills. Most of the others ate chicken liver pate.

 

Research – Global Green – Salmonella Innoculation – Salmongenics

Global Green EGGS

Global Green has acquired the world-wide exclusive rights to manufacture, distribute, market and sell Salmogenics™, a patented vaccine that helps to combat the dangerous effects of salmonella in humans and animals.

Salmogenics is in its last stages of testing and trials before the final USDA approval for its commercial application for becoming an approved vaccine for in ovo (injected into the egg) vaccination.

Research – Pecan Shell Extract – Anti – Listerial?

Journal of Food ScienceEurofins Food Testing UK

Growers and processors of USDA certified organic foods are in need of suitable organic antimicrobials. The purpose of the research reported here was to develop and test natural antimicrobials derived from an all-natural by-product, organic pecan shells. Unroasted and roasted organic pecan shells were subjected to solvent free extraction to produce antimicrobials that were tested against Listeria spp. and L. monocytogenes serotypes to determine the minimum inhibitory concentrations (MIC) of antimicrobials. The effectiveness of pecan shell extracts were further tested using a poultry skin model system and the growth inhibition of the Listeria cells adhered onto the skin model were quantified. The solvent free extracts of pecan shells inhibited Listeria strains at MICs as low as 0.38%. The antimicrobial effectiveness tests on a poultry skin model exhibited nearly a 2 log reduction of the inoculated cocktail mix of Listeria strains when extracts of pecan shell powder were used. The extracts also produced greater than a 4 log reduction of the indigenous spoilage bacteria on the chicken skin. Thus, the pecan shell extracts may prove to be very effective alternative antimicrobials against food pathogens and supplement the demand for effective natural antimicrobials for use in organic meat processing.

Research – Current Intervention Strategies for the Microbial Safety of Sprouts

Ingenta Connect

Sprouts have gained popularity worldwide due to their nutritional values and health benefits. The fact that their consumption has been associated with numerous outbreaks of foodborne illness threatens the $250 million market that this industry has established in the United States. Therefore, sprout manufacturers have utilized the U.S. Food and Drug Administration recommended application of 20,000 ppm of calcium hypochlorite solution to seeds before germination as a preventative method. Concentrations of up to 200 ppm of chlorine wash are also commonly used on sprouts. However, chlorine-based treatment achieves on average only 1- to 3-log reductions in bacteria and is associated with negative health and environmental issues. The search for alternative strategies has been widespread, involving chemical, biological, physical, and hurdle processes that can achieve up to 7-log reductions in bacteria in some cases. The compilation here of the current scientific data related to these techniques is used to compare their efficacy for ensuring the microbial safety of sprouts and their practicality for commercial producers. Of specific importance for alternative seed and sprout treatments is maintaining the industry-accepted germination rate of 95% and the sensorial attributes of the final product. This review provides an evaluation of suggested decontamination technologies for seeds and sprouts before, during, and after germination and concludes that thermal inactivation of seeds and irradiation of sprouts are the most practical stand-alone microbial safety interventions for sprout production.

Research – Listeria in Cheese sites – E.coli O157/Salmonella on Spinach

Science Direct

Inhibition of Escherichia coli O157:H7 and Salmonella enterica on spinach and identification of antimicrobial substances produced by a commercial Lactic Acid Bacteria food safety intervention
The microbiological safety of fresh produce is of concern for the U.S. food supply. Members of the Lactic Acid Bacteria (LAB) have been reported to antagonize pathogens by competing for nutrients and by secretion of substances with antimicrobial activity, including organic acids, peroxides, and antimicrobial polypeptides. The objectives of this research were to: (i) determine the capacity of a commercial LAB food antimicrobial to inhibit Escherichia coli O157:H7 and Salmonella enterica on spinach leaf surfaces, and (ii) identify antimicrobial substances produced in vitro by the LAB comprising the food antimicrobial. Pathogens were inoculated on freshly harvested spinach, followed by application of the LAB antimicrobial. Treated spinach was aerobically incubated up to 12 days at 7 °C and surviving pathogens enumerated via selective/differential plating. l-Lactic acid and a bacteriocin-like inhibitory substance (BLIS) were detected and quantified from cell-free fermentates obtained from LAB-inoculated liquid microbiological medium. Application of 8.0 log10 CFU/g LAB produced significant (p < 0.05) reductions in E. coli O157:H7 and Salmonella populations on spinach of 1.6 and 1.9 log10 CFU/g, respectively. It was concluded the LAB antimicrobial inhibited foodborne pathogens on spinach during refrigerated storage, likely the result of the production of metabolites with antimicrobial activity.

Mary Ann Leibert

Collaborative Survey on the Colonization of Different Types of Cheese-Processing Facilities with Listeria monocytogenes

Cross-contamination via equipment and the food-processing environment has been implicated as the main cause of Listeria monocytogenes transmission. The aim of this study, therefore, was to determine the occurrence and potential persistence of L. monocytogenes in 19 European cheese-processing facilities. A sampling approach in 2007–2008 included, respectively, 11 and two industrial cheese producers in Austria and the Czech Republic, as well as six Irish on-farm cheese producers. From some of the producers, isolates were available from sampling before 2007. All isolates from both periods were included in a strain collection consisting of 226 L. monocytogenes isolates, which were then typed by serotyping and pulsed-field gel electrophoresis (PFGE). In addition, metabolic fingerprints from a subset of isolates were obtained by means of Fourier-transform infrared (FTIR) spectroscopy. PFGE typing showed that six processing environments were colonized with seven persistent PFGE types of L. monocytogenes. Multilocus sequence typing undertaken on representatives of the seven persisting PFGE types grouped them into distinct clades on the basis of country and origin; however, two persistent strains from an Austrian and an Irish food processor were shown to be clonal. It was concluded that despite the fact that elaborate Hazard Analysis and Critical Control Point concepts and cleaning programs are applied, persistent occurrence of L. monocytogenes can take place during cheese making. L. monocytogenes sanitation programs could be strengthened by including rapid analytical tools, such as FTIR, which allow prescreening of potentially persistent L. monocytogenes contaminants.

 

Research Articles – Raw Milk – Antimicrobial Film Coatings to Control Listeria – E.col O157 and Salmonella Control by MAP and Polylysine – Gut Flora and Listeria Infection

Food Poisoning Bulletin

One of every six people who drinks raw milk gets food poisoning, according to a new study from officials at the Minnesota Department of Health.  That percentage is much higher than the number of cases reported in relation to outbreaks associated with raw milk and, researchers say, it’s increasing even as some states push to relax raw milk regulations.

Science Direct

Edible antimicrobial coating solutions incorporating chitosan, lauric arginate ester (LAE) and nisin were developed to reduce foodborne pathogen contamination on ready-to-eat (RTE) meats. RTE deli meat samples were directly coated with the solutions, or treated with solution-coated polylactic acid (PLA) films. The antimicrobial efficacy of the coatings and films against Listeria innocua inoculated onto the surface of RTE meat samples was investigated. Antimicrobial coatings with 1.94 mg/cm2 of chitosan and 0.388 mg/cm2 of LAE reduced L. innocua by ca. 4.5 log CFU/cm2. Nisin (486 IU/cm2) showed less effectiveness than LAE (0.388 mg/cm2) and addition of nisin to the antimicrobial coatings or films containing LAE (0.388 mg/cm2) did not enhance the total antimicrobial effectiveness. Combining antimicrobial coatings or films with flash pasteurization (FP), which uses short burst of steam under pressure, further reduced L. innocua, achieving over a 5 log reduction. There was no significant difference in the effectiveness of antimicrobial films versus the coatings (p > 0.05). These data show the potential use of antimicrobial packaging alone, or in combination with FP, in preventing foodborne illness due to post-processing contamination of RTE meat products.

Science Direct

Escherichia coli O157:H7 and Salmonella spp. often contaminate fresh beef. In Japan, an E coli outbreak caused by raw beef made 181 people ill and 5 individuals dead in 2011. Responding to this outbreak, an effective sterilization method for fresh beef is expected to be developed. In this study, ε-polylysine combined with CO2-packaging method was examined for controlling these pathogens in fresh beef. At an incubation temperature of 4 °C, approximately 4.3 log and 2.4 log reduction in bacterial numbers were observed after 7-day incubation for E. coli O157:H7 and Salmonella, respectively, in ε-polylysine-added beef. When effectiveness of CO2-packaging combined with ε-polylysine was investigated, CO2 did not have additional inhibiting effect on bacterial growth compared to only-ε-polylysine-treated samples when incubated at 4 °C. However, effectiveness of CO2 was observed when incubated at 10 °C where approximately 2.9 log and 4.4 log reduction in E. coli cell numbers were observed in only-ε-polylysine-treated samples and polylysine- and CO2-treated group, respectively, and approximately 1.7 log and 3.5 log reduction in Salmonella cell numbers were observed in only-ε-polylysine-treated samples and polylysine and CO2-treated group, respectively. This study confirmed that ε-polylysine or ε-polylysine combined with CO2 packaging are effective in preventing foodborne diseases caused by raw beef.

Food Poisoning Bulletin

The army of microbes that resides in the human gut may provide a powerful defense against Listeria infection, according to a study published in mBio, the online open-access journal of the American Society for Microbiology. The study, by a team of French researchers, shows that mice with germ-free intestines are more susceptible to Listeria infection than mice with the conventional intestinal microbes.

 

UK – FSA – Call For Tender – Effectiveness of Surface Treatment in Reducing Microbiological Contamination

FSAfood_standards_agency_logo

The Food Standards Agency is inviting tenders to carry out research on the effectiveness of surface treatment in reducing microbiological contamination of meat. This project only applies to meat intended for the production of raw or lightly cooked food.