Cantaloupes contaminated with Listeria and Salmonella have caused large scale of foodborne illness outbreaks in recent years. Prior research has shown that heat treatment can be used to reduce microbial contaminates on fruit surfaces. However, no study has evaluated the use of kitchen steamers for inactivating foodborne pathogens on cantaloupes. The purpose of this study was to test the sanitization efficacy of three models of electrical kitchen steamers (steamer‐A, ‐B, and ‐C) for eliminating foodborne pathogens on cantaloupe surfaces. Fresh cantaloupes were spot‐inoculated with Listeria monocytogens or Salmonella enterica and kept at 4 or 22 °C for a day before steam treatment for ≤100 s. Test results show that the surface of nonrefrigerated cantaloupes can be decontaminated (with ≥5.0 log reduction) within 60 s using any of the tested steamers. For refrigerated fruits (4 °C), however, only two steamers (steamer‐B and ‐C) decontaminated the cantaloupes within 60 s and they were unable to render the pathogens undetected in enriched samples. Salmonella was more susceptible than Listeria to steam treatment. In conclusion, electrical kitchen steamers can be utilized by consumers to sanitize whole cantaloupes prior to consumption. However, fruit temperature and steamer selection can influence the heating time required for surface sanitization.
Melons with netted rind surfaces, such as cantaloupes, likely have caused more outbreaks because their surface shelters pathogens from being eliminated by conventional washing and sanitization. As whole melons marketed at retail stores typically are not treated due to shelf‐life considerations, the safety of untreated melons can be of concern to consumers, especially among at‐risk populations. This study focused on the evaluation of the use of commercially available kitchen steamers in eliminating foodborne pathogens on cantaloupe surfaces. This study showed that kitchen steamers can be utilized to sanitize whole cantaloupes and also at household level, be a highly beneficial for the average concerned consumer to reduce the risk of pathogens on produce before consumption.
Posted in food contamination, Food Hygiene, Food Illness, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Pathogen, Food Safety, Food Technology, Food Testing, Listeria, Listeria welshimeri, Salmonella, Uncategorized
When testing conditions that prevail in fruit juice industry it was found that Listeria monocytogenes ATCC 7644 and a wild strain of Listeria innocua were highly hydrophobic, expressed swimming and twitching, co‐aggregated with yeast cells and produced exopolysaccharide, all characteristics that would favor the adhesive process. In mono‐species biofilms, L. innocua adhered on stainless steel at significantly higher counts than L. monocytogenes achieving values of 6.64 ± 0.01 and 5.80 ± 0.21 log CFU/cm2. In dual species biofilms with resident yeasts, L. innocua cells counts increase significantly in the presence of Candida tropicalis and Candida krusei and decrease significantly in the presence of Rhodotorula mucilaginosa and Candida kefyr. When cocultured with Candida tropicalis, the cell numbers of L. monocytogenes had a significant increase. These results revealed synergic and antagonistic interactions among species.
The study supports the plausibility that interactions between L. monocytogenes and members of resident microbiota, such as C. tropicalis, C. krusei, C. kefyr, and R. mucilaginosa, might play an important role for the survival and dissemination of L. monocytogenes. Apple juice processing conditions was used as simulation of the real condition in fruit juice processing environment and these results will alert to fruit juice industry to adopt the best cleaning and disinfecting practices against Listeria.
Posted in Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Pathogen, Food Safety, Food Testing, Listeria, Listeria monocytogenes, Listeria welshimeri, Uncategorized
WASHINGTON, Sept. 11, 2018 – CTI Foods LLC, an Owingsville, Ky., establishment, is recalling approximately 6,720 pounds of ready-to-eat (RTE) Philly Beef Steak products that may be adulterated with Listeria monocytogenes, the U.S. Department of Agriculture’s Food Safety and Inspection Service (FSIS) announced today.
The RTE Philly Beef Steak products were produced on Aug. 9, 2018. The following products are subject to recall: [View Labels (PDF only)]
- 672 cardboard cases labeled Classic Sysco having a NET WT of 10 lbs. The cardboard cases contain four 2.5 lb. bags of product. Both the box and the bags are labeled “FULLY COOKED PHILLY BEEF STEAK SLICED Caramel Color Added” with a package code of 4887097.
The products subject to recall bear establishment number “EST. 19085” inside the USDA mark of inspection. These items were shipped to a food service warehouse in Hamilton, Ohio and were further distributed to food service locations.
The problem was discovered on Sept. 7, 2018, when the establishment received results from their laboratory that four finished product samples from their Aug. 9, 2018 production were potentially positive for Listeria monocytogenes.
There have been no confirmed reports of adverse reactions due to consumption of these products. Anyone concerned about an injury or illness should contact a healthcare provider.
Posted in food contamination, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Pathogen, food recall, Food Safety, Food Safety Alert, Food Testing, Listeria monocytogenes, Listeria welshimeri, Uncategorized
Journal of Food Protection
Listeria species are ubiquitous in nature and can adapt to survive in a variety of niches, including food processing environments. Listeria species that colonize these environments may also have the potential to persist. Food safety strategies designed to manage these niches include regular cleaning and disinfection with proven sanitizers containing biocide-active compounds. Typically, these sanitizers are effective against bacteria growing under planktonic conditions, but their efficacy may be compromised when bacteria are contained in biofilms. The susceptibility of persistent Listeria isolates, i.e., those capable of forming biofilms, to a selection of sanitizers was investigated. A quaternary ammonium compound–based sanitizer was the biocide most effective against planktonic bacteria, with a MIC of 0.0015 to 0.006%. In contrast, ethanol-based sanitizers were the least effective. Although, no triclosan tolerance was observed for planktonic Listeria isolates, triclosan was the only biocide that resulted in a significant biomass reduction. Differences between Listeria species were observed; L. monocytogenes and L. welshimeri biofilms were more tolerant to quaternary ammonium compound–based sanitizers than were L. innocua biofilms. These findings extend our understanding of the application of commonly used sanitizers in the food industry and the efficacy of these sanitizers against Listeria species and their associated biofilms.
Listeria is a harmful bacteria. Listeria thrives in ready-to-eat foods that have already been cooked when purchased.
Listeria monocytogenes (listeria) is a bacterium that causes an illness called Listeriosis. Cases of foodborne illness from listeria are rare, but can involve serious symptoms and even death in certain groups of people. These include:
- people with weakened immune systems, such as those suffering from cancer
- pregnant women and their unborn babies
- newborn babies
- elderly people
The microbiological safety of fresh produce is monitored almost exclusively by culture-based detection methods. However, bacterial food-borne pathogens are known to enter a viable-but-nonculturable (VBNC) state in response to environmental stresses such as chlorine, which is commonly used for fresh produce decontamination. Here, complete VBNC induction of green fluorescent protein-tagged Listeria monocytogenes and Salmonella enterica serovar Thompson was achieved by exposure to 12 and 3 ppm chlorine, respectively. The pathogens were subjected to chlorine washing following incubation on spinach leaves. Culture data revealed that total viable L. monocytogenes and Salmonella Thompson populations became VBNC by 50 and 100 ppm chlorine, respectively, while enumeration by direct viable counting found that chlorine caused a <1-log reduction in viability. The pathogenicity of chlorine-induced VBNC L. monocytogenes and Salmonella Thompson was assessed by using Caenorhabditis elegans. Ingestion of VBNC pathogens by C. elegans resulted in a significant life span reduction (P = 0.0064 and P < 0.0001), and no significant difference between the life span reductions caused by the VBNC and culturable L. monocytogenes treatments was observed. L. monocytogenes was visualized beyond the nematode intestinal lumen, indicating resuscitation and cell invasion. These data emphasize the risk that VBNC food-borne pathogens could pose to public health should they continue to go undetected.
IMPORTANCE Many bacteria are known to enter a viable-but-nonculturable (VBNC) state in response to environmental stresses. VBNC cells cannot be detected by standard laboratory culture techniques, presenting a problem for the food industry, which uses these techniques to detect pathogen contaminants. This study found that chlorine, a sanitizer commonly used for fresh produce, induces a VBNC state in the food-borne pathogens Listeria monocytogenes and Salmonella enterica. It was also found that chlorine is ineffective at killing total populations of the pathogens. A life span reduction was observed in Caenorhabditis elegans that ingested these VBNC pathogens, with VBNC L. monocytogenes as infectious as its culturable counterpart. These data show that VBNC food-borne pathogens can both be generated and avoid detection by industrial practices while potentially retaining the ability to cause disease.
RASFF – Aflatoxins (B1 = 49; Tot. = 60 µg/kg – ppb) in groundnuts from Brazil in the Netherlands
RASFF – Aflatoxins (B1 = 18; Tot. = 21 µg/kg – ppb) in groundnuts from Brazil in the Netherlands
RASFF – Listeria monocytogenes (<10; 110; <10; 91; 131 CFU/g) in chilled smoked salmon from France in Italy
RASFF – Salmonella spp. (presence /25g) in frozen chicken meat from Brazil in Spain
RASFF – Salmonella enteritidis (present in 5 out of 5 samples /25g) in chilled chicken quarters from Slovakia, with raw material from Poland in Slovakia
Posted in Aflatoxin, Bacteria, Eurofins Laboratories, Food Hygiene, Food Inspections, Food Micro Blog, Food Microbiology, Food Poisoning, Food Safety, Food Safety Alert, Food Testing, Laboratory, Listeria monocytogenes, Listeria welshimeri, Microbiology, Pathogen, RASFF, Recall, Salmonella
Tagged listeria monocytogenes, salmonella spp