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Category Archives: Decontamination Microbial
Research – Leveraging Plasma-Activated Seawater for the Control of Human Norovirus and Bacterial Pathogens in Shellfish Depuration
Cold plasma is a promising alternative for water treatment owing to pathogen control and a plethora of issues in the agriculture and food sectors. Shellfish pose a serious risk to public health and are linked to large viral and bacterial outbreaks. Hence, current European regulations mandate a depuration step for shellfish on the basis of their geographical growth area. This study investigated the inactivation of relevant viral and bacterial pathogens of three plasma-activated seawaters (PASWs), and their reactive oxygen and nitrogen species (RONS) composition, as being primarily responsible for microbial inactivation. Specifically, F-specific (MS2) and somatic (φ174) bacteriophage, cultivable surrogate (murine norovirus, MNV, and Tulane virus, TV), and human norovirus (HuNoV GII.4) inactivation was determined using plaque counts and infectivity assays, including the novel human intestinal enteroid (HIE) model for HuNoV. Moreover, the kinetic decay of Escherichia coli, Salmonella spp., and Vibrio parahaemolyticus was characterized. The results showed the complete inactivation of phages (6–8 log), surrogates (5–6 log), HuNoV (6 log), and bacterial (6–7 log) pathogens within 24 h while preventing cytotoxicity effects and preserving mussel viability. Nitrites (NO2−) were found to be mostly correlated with microbial decay. This research shows that PASWs are a suitable option to depurate bivalve mollusks and control the biohazard risk linked to their microbiological contamination, either viral or bacterial.
Posted in Cold Atmospheric Plasma, Cold Plasma, Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbial Spoilage, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk
Research – Low-Temperature Plasma used to remove E. coli from hydroponically grown crops
A group led by researchers at Nagoya University and Meijo University in Japan has developed a disinfection technology that uses low-temperature plasma generated by electricity to cultivate environmentally friendly hydroponically grown crops. This innovative technology sterilizes the crops, promoting plant growth without the use of chemical fertilizers. Their findings appeared in Environmental Technology & Innovations.
In hydroponic agriculture, farmers cultivate plants by providing their roots with a nutrient solution.
However, the nutrient solution can become infected with pathogenic E. coli strains, contaminating the crop and leading to foodborne illnesses.
Posted in Decontamination Microbial, E.coli, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Technology, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Research, Technology
USA – Spring Holidays are a Treat, Don’t Let Bacteria Ruin Your Feast
It’s a special time of year, where friends and family from coast to coast will gather for a rare occurrence: the three major holidays of Easter, Eid, and Passover all falling within the spring season. People who observe these faiths will gather with friends and family to enjoy traditional meals.
“The holiday season is a special time to gather with friends and family and enjoy traditional meals,” said USDA Under Secretary for Food Safety Dr. Emilio Esteban. “Whether you’re celebrating your Easter dinner with ham, Eid lunch with lamb, or Seder meal with brisket, remember to keep food safety at the forefront.”
These holiday meals often have cultural significance and may require foods to be prepared early, so that religious obligations are completed prior to gathering and enjoying the meals. This provides an extended opportunity for foodborne illness to ruin major holiday dishes. The U.S. Department of Agriculture’s (USDA) Food Safety and Inspection Service (FSIS) wants to keep your celebrations going with some tips on how not to be at risk for foodborne illness.
Here are six pieces of advice from the USDA to keep your holiday meals safe:
#1 Keep Hands Clean Before, During, and After Food Prep
Handwashing is the first step to avoiding foodborne illness. In a recent USDA study, 96% of handwashing attempts failed due to not including all the necessary steps. Wash your hands for at least 20 seconds with soap and water before and after handling food and when switching between ingredients.
#2 Prevent Cross-Contamination of Surfaces and Foods
Cross-contamination is the spread of bacteria from raw meat and poultry onto ready-to-eat food, surfaces, and utensils. Avoid this by using separate cutting boards — one for raw meat and poultry, and another for ready-to-eat foods such as fruits and vegetables. Clean and sanitize any areas where meat and poultry have touched before and after cooking. Cleaning with soap and water physically removes the germs, and sanitizing kills any that may remain. Many different sanitizers can be used: an easy homemade version is to make a solution of 1 tablespoon of liquid chlorine bleach per gallon of water, or you can use a commercial sanitizer or sanitizing wipe.
#3 Thaw Meat and Poultry Safely
Leaving any frozen package of meat or poultry for more than two hours on the counter at room temperature is dangerous. Even though the center of the package may still be frozen, the outer layer of the food is in the “Danger Zone” between 40 and 140 F — a temperature where foodborne bacteria multiply rapidly and cause foodborne illness. Plan for refrigerator, cold water, or microwave thawing instead.
#4 Cook to a Safe Internal Temperature
Color is never a reliable indicator of safety and doneness. Use a food thermometer to ensure the following safe internal temperatures:
- Cook fish to 145 F.
- Cook beef, pork, lamb and veal steaks, chops and roasts to 145 F. For safety and quality, allow meat to rest for at least three minutes before carving or consuming.
- Ham, fresh or smoked (uncooked): 145 F
- Cook ground meats, including beef, pork, lamb and veal to 160 F.
- Cook egg dishes to 160 F.
- Cook poultry (whole or ground) to 165 F.
When reheating already cooked meat, poultry or egg items that have cooled, they should be reheated to at least 165 °F. USDA recommends reheating cooked meat, poultry or egg products on the stove top, in the oven, or in a microwave.
#5 Keep Hot Foods Hot, and Cold Foods Cold
Food should not be left in the Danger Zone for more than two hours (called the two-hour rule). After two hours, bacteria can reach dangerous levels that can cause foodborne illness. Perishable foods, such as kebabs or brisket, should be discarded if left out for longer than two hours.
To prevent food waste, refrigerate or freeze perishable items within two hours, or keep hot foods hot and cold foods cold. Keep cold foods at a temperature of 40 F or below by keeping food nestled in ice or refrigerated until ready to serve. Keep hot foods at a temperature of 140 F or above by placing food in a preheated oven, warming trays, chafing dishes or slow cookers.
#6 Use or Freeze Leftovers within Four Days
Leftovers (including appetizers, side dishes and main courses) should be stored within two hours of cooking. Divide leftovers into smaller portions and refrigerate or freeze them in shallow containers, which helps leftovers cool quicker than storing them in large quantities. Place leftovers into airtight containers to help keep bacteria out and retain moisture.
Leftovers can be kept in the refrigerator for three to four days or frozen for three to four months. Although safe indefinitely, frozen leftovers can lose moisture and flavor when stored for longer times in the freezer.
Research – A new Rogue-like Escherichia phage UDF157lw to control Escherichia coli O157:H7
Introduction: Shiga toxin-producing Escherichia coli (STEC) O157:H7 is one of the notorious foodborne pathogens causing high mortality through the consumption of contaminated food items. The food safety risk from STEC pathogens could escalate when a group of bacterial cells aggregates to form a biofilm. Bacterial biofilm can diminish the effects of various antimicrobial interventions and enhance the pathogenicity of the pathogens. Therefore, there is an urgent need to have effective control measurements. Bacteriophages can kill the target bacterial cells through lytic infection, and some enzymes produced during the infection have the capability to penetrate the biofilm for mitigation compared to traditional interventions. This study aimed to characterize a new Escherichia phage vB_EcoS-UDF157lw (or UDF157lw) and determine its antimicrobial efficacy against E. coli O157:H7.
Posted in Bacteriophage, Biofilm, Decontamination Microbial, E.coli, E.coli O157, E.coli O157:H7, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Pathogen, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Pathogen, pathogenic, Phage, Research, STEC, STEC E.coli
Research – Tomato juice’s antimicrobial properties can kill Salmonella
Tomato juice can kill Salmonella Typhi and other bacteria that can harm people’s digestive and urinary tract health, according to research published this week in Microbiology Spectrum, a journal of the American Society for Microbiology. Salmonella Typhi is a deadly human-specific pathogen that causes typhoid fever.
“Our main goal in this study was to find out if tomato and tomato juice can kill enteric pathogens, including Salmonella Typhi, and if so, what qualities they have that make them work,” said principal study investigator Jeongmin Song, Ph.D., Associate Professor, Department of Microbiology & Immunology, Cornell University.
Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbial Spoilage, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Research, Salmonella
Research – The Investigation of Changes in Bacterial Community of Pasteurized Milk during Cold Storage
Abstract
The quality of pasteurized milk is commonly assessed through microbiological analysis, with variations in storage conditions significantly impacting the suppression of bacterial growth throughout the milk’s shelf life. This study investigated the dynamics of total bacterial counts (TBCs) and bacterial community shifts in milk that underwent pasteurization at 80 °C for 15 s. The milk was subsequently stored at 4 °C for varying intervals of 1, 4, 7, 10, 13, and 16 days. Culture-based testing revealed a significant TBC increase during the storage period spanning 1 to 16 days (up to −log10 4.2 CFU/mL at day 16). The TBC in pasteurized milk exhibited accelerated microbial growth from day 13 onwards, ultimately peaking on day 16. Bacillus was detected through 16S rRNA identification. Principal component analysis demonstrated a significant impact of storage time on bacterial communities in pasteurized milk. Analysis of bacterial diversity revealed a negative correlation between the Shannon index and the duration of pasteurized milk storage. Using high-throughput sequencing, Streptococcus and Acinetobacter were detected as prevalent bacterial genera, with Streptococcus dysgalactiae and Streptococcus uberis showing as dominant taxa. The presence of Streptococcus dysgalactiae and Streptococcus uberis in pasteurized milk might be attributed to the initial contamination from raw milk with mastitis. This study offers new evidence of the prevalence of bacterial community in pasteurized milk, thereby adding value to the enhancement of quality control and the development of strategies for reducing microbial risks.
Posted in Acinetobacter, Decontamination Microbial, Faecal Streptpcocci, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, microbial contamination, Microbial growth, Microbial Spoilage, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Pasteurisation
Risk ranking of foodborne diseases in Denmark: Reflections on a national burden of disease study
Abstract
National burden of foodborne disease (FBD) studies are useful for risk ranking and identifying priorities for food safety resource allocation. FBD are caused by microbiological and chemical hazards, which have different incidence and mortality, and lead to health-outcomes varying in severity and duration. Due to their different origins and characteristics, distinct prevention strategies can be implemented. We present the Danish initiative to estimate the burden of FBD caused by pathogens and chemicals. We describe the methodological approaches and data used, present an overview of results for the reference year 2019, and discuss lessons learned throughout more than seven years of the project. Our estimates show that the leading causes of FBD in Denmark include both pathogens and chemicals, suggesting that food safety strategies should be diversified to tackle different issues. The experiences with this national study are useful for other countries planning to implement a burden of FBD study.
Research – Inhibition of Food-Borne Pathogen Growth and Biogenic Amine Synthesis by Spice Extracts
Food-borne pathogens and their toxins cause significant health problems in humans. Formation of biogenic amines (BAs) produced by microbial decarboxylation of amino acids in food is undesirable because it can induce toxic effects in consumers. Therefore, it is crucial to investigate the effects of natural additives with high bioactivity like spice extracts to inhibit the growth of these bacteria and the formation of BAs in food. In the present study, the antibacterial effects of diethyl ether spice (sumac, cumin, black pepper, and red pepper) extracts at doses of 1% (w/v) on Gram-positive (Staphylococcus aureus and Enterococcus faecalis) and Gram-negative (Klebsiella pneumoniae, Pseudomonas aeruginosa, Campylobacter jejuni, Aeromonas hydrophila, Salmonella Paratyphi A, and Yersinia enterocolitica) food-borne pathogen bacterial strains (FBP) were established. In addition, the accumulation of ammonia (AMN), trimethylamine (TMA), and biogenic amines (BAs) in tyrosine decarboxylase broth (TDB) was investigated by using high performance liquid chromatography (HPLC). Sumac extract exhibited the highest antibacterial potential against all FBPs, followed by cumin and peppers. AMN (570.71 mg/L) and TMA (53.66 mg/L) production were strongly inhibited by sumac extract in the levels of 55.10 mg/L for Y. enterocolitica and 2.76 mg/L for A. hydrophila, respectively. With the exception of S. aureus, black pepper dramatically reduced the synthesis of putrescine, serotonin, dopamine, and agmatine by FBP especially for Gram-negative ones. Furthermore, sumac extracts inhibited histamine and tyramine production by the majority of FBP. This research suggests the application of sumac extracts as natural preservatives for inhibiting the growth of FBPs and limiting the production of AMN, TMA, and BAs.
Posted in Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Food Pathogen, microbial contamination, Microbial growth, Microbial Spoilage, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Pathogen, Research
Research – Process Hygiene Criterion for Campylobacter and Number of Campylobacter Enteritis Cases in Northwest Germany
Abstract
Campylobacteriosis is the most commonly reported bacterial foodborne disease in the European Union. Its transmission is often associated with the consumption of poultry meat. In 2018, Regulation (EC) No. 2017/1495 introduced a process hygiene criterion and with this, the testing requirements for Campylobacter. The results of microbiological testing for Campylobacter of chicken carcass neck skin samples from several slaughter lines in Northwest Germany collected by the food business operators and contamination levels (cfu/g Campylobacter) of these samples were analysed from 2018 to 2021. Classification into three different categories was made based on contamination levels. The proportion of highly contaminated (category three) neck samples (>1000 cfu/g) decreased from 2018 to 2021. Our analysis showed a relationship between the number of neck samples with high Campylobacter contamination levels (>1000 cfu/g) and human cases in Northwest Germany. Spearman’s rank test (p < 0.01) showed a higher correlation in 2018 (0.66) and 2019 (0.58) compared to 2020 and 2021. Campylobacter enteritis cases in Northwest Germany stayed at a low level in 2020 and 2021. It remains unclear whether the decrease in reported Campylobacter enteritis cases is related to a decrease in Campylobacter levels on chicken carcasses or due to other reasons like underreporting during the COVID-19 pandemic, and therefore must be investigated in further analyses.
Posted in Campylobacter, Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, microbial contamination, Microbial growth, Microbial Spoilage, Microbiology, Microbiology Investigations, Microbiology Risk, Research
EU – Nestle recalls infant formula due to Cronobacter risk
Nestlé has recalled infant formula in several countries because of the potential presence of Cronobacter sakazakii.
The affected product is EXPERT PRO HA 1, which has different batch numbers depending on the country it was marketed.
Infant formula from Germany was sent to Bulgaria, Croatia, Czech Republic, Denmark, Finland, Greece, Hungary, Norway, Poland, Romania, Slovakia, and Sweden, according to a notice on the EU’s Rapid Alert System for Food and Feed (RASFF).
In Denmark, it was sold to a few stores and online webshops from Nov. 29 to Dec. 13, 2023. The Danish version NAN EXPERTPRO HA 1 800-grams has article number 12489069, batch number 33120742C2, and an expiration date of 11.2025.
In Norway, the recall applies to 90 units sold to pharmacies, including online pharmacies. NAN EXPERTPRO HA 1 has article number 12468459, batch number 33120742C1, and a best-before date of 11.2025. Products were distributed between Dec. 7 and 12, 2023.
In the Czech Republic and Slovakia, BEBA EXPERT PRO HA 1, 800-gram with batch number 33140742F3 is involved.
The product’s batch number and expiration date are at the bottom of the can. No other products or batches are affected.
No positive products or illnesses
Posted in Cronobacter sakazakii, cross contamination, Decontamination Microbial, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Testing, microbial contamination, Microbial Spoilage, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk
