Tag Archives: Campylobacter jejuni

Research – Antimicrobial Resistance Campylobacter on Poultry – Microbial Biofilms in Seafood

Science Direct –  Five-year study on prevalence and antimicrobial resistance of Campylobacter from poultry carcasses in Poland

During 2009–2013 a total of 2114 swab samples collected from broiler carcasses in all 16 voivodeships (administrative districts) of Poland were examined for the presence of Campylobacter jejuni and Campylobacter coli. The antimicrobial resistance of the isolates to ciprofloxacin, tetracycline and erythromycin using the MIC method was also tested. It was found that 1151 (54.4%) carcasses were contaminated with Campylobacter, with 50% of C. jejuni and C. coli species isolated from positive samples. The temporal trend in the prevalence of Campylobacter-positive samples demonstrated that the highest percentage of carcasses was contaminated during the first year of the survey (70.5%) whereas in the last year (2013) only 36.3% of broilers contained these bacteria. Antimicrobial resistance analysis showed that overall 939 (81.6%) of isolates were resistant to ciprofloxacin, 646 (56.1%) to tetracycline but only 28 (2.4%) to erythromycin. Significant differences in resistance profiles between C. jejuni and C. coli were observed with greater resistance level observed in the latter species. Furthermore, a significant increase in the percentage of C. jejuni resistant to ciprofloxacin (from 59.6% in 2009 to 85.9% in 2014) and to tetracycline (from 23.2% to 70.4%, respectively) was identified. Only 20 (1.7%) Campylobacter isolates displayed a multiresistance pattern.

Science Direct – Microbial biofilms in seafood: A food-hygiene challenge

Seafood forms a part of a healthy diet. However, seafood can be contaminated with foodborne pathogens, resulting in disease outbreaks. Because people consume large amounts of seafood, such disease outbreaks are increasing worldwide. Seafood contamination is largely due to the naturally occurring phenomenon of biofilm formation. The common seafood bacterial pathogens that form biofilms are Vibrio spp., Aeromonas hydrophila, Salmonella spp., and Listeria monocytogenes. As these organisms pose a global health threat, recent research has focused on elucidating methods to eliminate these biofilm-forming bacteria from seafood, thereby improving food hygiene. Therefore, we highlight recent advances in our understanding of the underlying molecular mechanisms of biofilm formation, the factors that regulate biofilm development and the role of quorum sensing and biofilm formation in the virulence of foodborne pathogens. Currently, several novel methods have been successfully developed for controlling biofilms present in seafood. In this review, we also discuss the epidemiology of seafood-related diseases and the novel methods that could be used for future control of biofilm formation in seafood.

RASFF Alerts – Histamine – Tuna – Moulds – Cheese – Listeria – Raw Milk Cheese – E.coli – Clams – Campylobacter – Chicken

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RASFF-histamine (from 310 to 334 mg/kg – ppm) in frozen yellowfin tuna from India in France

RASFF-high count of moulds (< 100; <100; 100000; 85000; <100; <100; <100; 2000; 360; <100 CFU/g) in ground cheese from Italy in Italy

RASFF-Listeria monocytogenes (>6000 CFU/g) in raw cow’s milk cheese from France in France

RASFF-too high count of Escherichia coli (9200 MPN/100g) in live clams (Ruditapes philippinarum) from Italy in Italy

RASFF -Campylobacter jejuni (500/1200/500/300/100/1300/300/900/100/200/100/700 CFU/g) in chickenbreast fillets from Poland in Denmark

RASFF Alerts – November – Chicken

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RASFF-Campylobacter coli (100-6000 CFU/g) and Campylobacter jejuni (200 CFU/g) in whole chilled chicken from France in Denmark

Research – Effect of Packaging and Temperature on Survival Kinetics of Campylobacter jejuni on Precooked Chicken Breast

Wiley Online Library imagesCAYZ5I84

The survival ability of Campylobacter jejuni in poultry has been a major public health risk because of the low infectious dose. The objective of this study was to investigate the effects of different packaging condition and temperature on the survival kinetics of C. jejuni ATCC 33291 on precooked chicken breast. Survival kinetics of C. jejuni were also compared with those of C. coli ATCC 43486. Inoculated chicken breasts with 5.0–6.0 log cfu/g were packed in three different ways (aerobic, semi-aerobic or vacuum), which were stored at 4, 10, 17, 24, 30 and 36C. Survival curves were fitted to Baranyi model to obtain the values of lag time and death rate of C. jejuni. During storage, the greatest reduction of C. jejuni (4.316 log cfu/g) was observed in aerobic packaged chicken breast at 24C, while the least reduction of C. jejuni (1.031 log cfu/g) was observed in vacuum-packaged chicken breast at 4C. Lag time was affected by packing method, temperature and strain. Similar survival behavior of C. coli to C. jejuni was observed only at 10C. At the temperatures above 24C, lag phase of C. jejuni was only observed in semi-aerobic or vacuum-packed chicken breasts, indicating that C. jejuni survived better than C. coli, for which no lag phase was observed. The results indicated that vacuum or semi-aerobic packaging on poultry product at 4C, which is the most popular method at retail markets, created the highest risk for C. jejuni survival.

RASFF Alerts – Campylobacter – Chicken – Bacillus cereus Toxin – Rocket

RASFF – Campylobacter coli (8 of 12 samples /25g) and Campylobacter jejuni (4 out of 12 samples /25g) in chilled boneless skinless chicken breast fillets from Poland in Denmark

RASFF – Bacillus cereus diarrheal enterotoxin (17000 CFU/g) in packed rocket from the Netherlands in Finland

Research Campylobacter Updates

Eurosurveillance

Differentiation between travel-related and domestic cases of infectious disease is important in managing risk. Incubation periods of cases from several outbreaks of campylobacteriosis in Canada, Europe, and the United States with defined exposure time of less than 24 hours were collated to provide information on the incubation period distribution. This distribution was consistent across the varied outbreaks considered, with 84% (702/832) of cases having an incubation period of four days or less and 1% having an incubation period of eight days or more. The incubation period distribution was incorporated into a model for the number of travel-related cases presenting with symptom onset at given dates after return to their country of residence. Using New Zealand notification data between 2006 and 2010 for cases who had undertaken foreign travel within 10 days prior to symptom onset, we found that 29.6% (67/227 cases; 95% confidence interval (CI): 28.3–30.8%) of these cases were likely to have been domestic cases. When cases with symptom onset prior to arrival were included, the probable domestic cases represented 11.8% (67/571; 95% CI: 11.2–12.3%). Consideration of incubation time distributions and consistent collection of travel start/end dates with symptom onset dates would assist attribution of cases to foreign travel.

Clincal Infectious Diseases

Campylobacteriosis in humans, caused by Campylobacter jejuni and C. coli, is the most common recognized bacterial zoonosis in the EU and US. The acute phase is characterized by gastro intestinal  symptoms. The long-term sequelae (Guillain-Barré Syndrome, reactive arthritis and post-infectious irritable bowel syndrome)  contribute considerably to the disease burden. Attribution studies identified poultry as the reservoir responsible for up         to 80% of the human Campylobacter infections. In the EU, an estimated 30% of the human infections is associated with consumption and preparation of poultry  meat. Until now, interventions in the poultry meat production chain have not been effectively introduced except for targeted  interventions in Iceland and New Zealand. Intervention measures (e.g. biosecurity) have limited effect or are hampered by  economic aspects or consumer acceptance. In the future a multi-level approach should be followed, aiming at reducing the level of contamination of consumer products rather than complete absence of Campylobacter.

CDC  – UK Duck Liver Campylobacter Outbreak

Campylobacter­ spp.–related gastroenteritis in diners at a catering college restaurant was associated with consumption of duck liver pâté. Population genetic analysis indicated that isolates from duck samples were typical of isolates from farmed poultry. Campylobacter spp. contamination of duck liver may present a hazard similar to the increasingly recognized contamination of chicken liver.

 

 

RASFF Alerts – Campylobacter – Chicken

RASFF -Campylobacter (between 400 and 17000 CFU/g) in chilled chicken thighs from Poland In Denmark

RASFF -Campylobacter jejuni (100 – 5.300 /g) in chilled whole chicken from Germany