Category Archives: Salmonella

Research – Pathogen Adaptation – Salmonella – Staphylococcus aureus

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Bacteria can evolve rapidly to adapt to environmental change. When the “environment” is the immune response of an infected host, this evolution can turn harmless bacteria into life-threatening pathogens. A study published on December 12 in PLOS Pathogens provides insight into how this happens.

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A protein in Salmonella inactivates mast cells — critical players in the body’s fight against bacteria and other pathogens — rendering them unable to protect against bacterial spread in the body, according to researchers at Duke Medicine and Duke-National University of Singapore (Duke-NUS).

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Scientists have used a new method to map the response of every salmonella gene to conditions in the human body, providing new insight into how the bacteria triggers infection.

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Scientists at the Stanford University School of Medicine have revealed that formerly overlooked sites deep inside the nose may be reservoirs for Staphylococcus aureus, a major bacterial cause of disease.

Research – Listeria in Cheese sites – E.coli O157/Salmonella on Spinach

Science Direct

Inhibition of Escherichia coli O157:H7 and Salmonella enterica on spinach and identification of antimicrobial substances produced by a commercial Lactic Acid Bacteria food safety intervention
The microbiological safety of fresh produce is of concern for the U.S. food supply. Members of the Lactic Acid Bacteria (LAB) have been reported to antagonize pathogens by competing for nutrients and by secretion of substances with antimicrobial activity, including organic acids, peroxides, and antimicrobial polypeptides. The objectives of this research were to: (i) determine the capacity of a commercial LAB food antimicrobial to inhibit Escherichia coli O157:H7 and Salmonella enterica on spinach leaf surfaces, and (ii) identify antimicrobial substances produced in vitro by the LAB comprising the food antimicrobial. Pathogens were inoculated on freshly harvested spinach, followed by application of the LAB antimicrobial. Treated spinach was aerobically incubated up to 12 days at 7 °C and surviving pathogens enumerated via selective/differential plating. l-Lactic acid and a bacteriocin-like inhibitory substance (BLIS) were detected and quantified from cell-free fermentates obtained from LAB-inoculated liquid microbiological medium. Application of 8.0 log10 CFU/g LAB produced significant (p < 0.05) reductions in E. coli O157:H7 and Salmonella populations on spinach of 1.6 and 1.9 log10 CFU/g, respectively. It was concluded the LAB antimicrobial inhibited foodborne pathogens on spinach during refrigerated storage, likely the result of the production of metabolites with antimicrobial activity.

Mary Ann Leibert

Collaborative Survey on the Colonization of Different Types of Cheese-Processing Facilities with Listeria monocytogenes

Cross-contamination via equipment and the food-processing environment has been implicated as the main cause of Listeria monocytogenes transmission. The aim of this study, therefore, was to determine the occurrence and potential persistence of L. monocytogenes in 19 European cheese-processing facilities. A sampling approach in 2007–2008 included, respectively, 11 and two industrial cheese producers in Austria and the Czech Republic, as well as six Irish on-farm cheese producers. From some of the producers, isolates were available from sampling before 2007. All isolates from both periods were included in a strain collection consisting of 226 L. monocytogenes isolates, which were then typed by serotyping and pulsed-field gel electrophoresis (PFGE). In addition, metabolic fingerprints from a subset of isolates were obtained by means of Fourier-transform infrared (FTIR) spectroscopy. PFGE typing showed that six processing environments were colonized with seven persistent PFGE types of L. monocytogenes. Multilocus sequence typing undertaken on representatives of the seven persisting PFGE types grouped them into distinct clades on the basis of country and origin; however, two persistent strains from an Austrian and an Irish food processor were shown to be clonal. It was concluded that despite the fact that elaborate Hazard Analysis and Critical Control Point concepts and cleaning programs are applied, persistent occurrence of L. monocytogenes can take place during cheese making. L. monocytogenes sanitation programs could be strengthened by including rapid analytical tools, such as FTIR, which allow prescreening of potentially persistent L. monocytogenes contaminants.

 

Research Articles – Raw Milk – Antimicrobial Film Coatings to Control Listeria – E.col O157 and Salmonella Control by MAP and Polylysine – Gut Flora and Listeria Infection

Food Poisoning Bulletin

One of every six people who drinks raw milk gets food poisoning, according to a new study from officials at the Minnesota Department of Health.  That percentage is much higher than the number of cases reported in relation to outbreaks associated with raw milk and, researchers say, it’s increasing even as some states push to relax raw milk regulations.

Science Direct

Edible antimicrobial coating solutions incorporating chitosan, lauric arginate ester (LAE) and nisin were developed to reduce foodborne pathogen contamination on ready-to-eat (RTE) meats. RTE deli meat samples were directly coated with the solutions, or treated with solution-coated polylactic acid (PLA) films. The antimicrobial efficacy of the coatings and films against Listeria innocua inoculated onto the surface of RTE meat samples was investigated. Antimicrobial coatings with 1.94 mg/cm2 of chitosan and 0.388 mg/cm2 of LAE reduced L. innocua by ca. 4.5 log CFU/cm2. Nisin (486 IU/cm2) showed less effectiveness than LAE (0.388 mg/cm2) and addition of nisin to the antimicrobial coatings or films containing LAE (0.388 mg/cm2) did not enhance the total antimicrobial effectiveness. Combining antimicrobial coatings or films with flash pasteurization (FP), which uses short burst of steam under pressure, further reduced L. innocua, achieving over a 5 log reduction. There was no significant difference in the effectiveness of antimicrobial films versus the coatings (p > 0.05). These data show the potential use of antimicrobial packaging alone, or in combination with FP, in preventing foodborne illness due to post-processing contamination of RTE meat products.

Science Direct

Escherichia coli O157:H7 and Salmonella spp. often contaminate fresh beef. In Japan, an E coli outbreak caused by raw beef made 181 people ill and 5 individuals dead in 2011. Responding to this outbreak, an effective sterilization method for fresh beef is expected to be developed. In this study, ε-polylysine combined with CO2-packaging method was examined for controlling these pathogens in fresh beef. At an incubation temperature of 4 °C, approximately 4.3 log and 2.4 log reduction in bacterial numbers were observed after 7-day incubation for E. coli O157:H7 and Salmonella, respectively, in ε-polylysine-added beef. When effectiveness of CO2-packaging combined with ε-polylysine was investigated, CO2 did not have additional inhibiting effect on bacterial growth compared to only-ε-polylysine-treated samples when incubated at 4 °C. However, effectiveness of CO2 was observed when incubated at 10 °C where approximately 2.9 log and 4.4 log reduction in E. coli cell numbers were observed in only-ε-polylysine-treated samples and polylysine- and CO2-treated group, respectively, and approximately 1.7 log and 3.5 log reduction in Salmonella cell numbers were observed in only-ε-polylysine-treated samples and polylysine and CO2-treated group, respectively. This study confirmed that ε-polylysine or ε-polylysine combined with CO2 packaging are effective in preventing foodborne diseases caused by raw beef.

Food Poisoning Bulletin

The army of microbes that resides in the human gut may provide a powerful defense against Listeria infection, according to a study published in mBio, the online open-access journal of the American Society for Microbiology. The study, by a team of French researchers, shows that mice with germ-free intestines are more susceptible to Listeria infection than mice with the conventional intestinal microbes.

 

RASFF Alerts – Listeria – Herring Fillets – E.coli – Clams – Salmonella – Spinach

RASFF -Listeria monocytogenes (< 10 CFU/g) in chilled herring fillets in oil from the Netherlands in France

RASFF -high count of Escherichia coli (1300 MPN/100g) in live clams from Croatia in Italy

RASFF -Salmonella poona in fresh water spinach from Sri Lanka in Finland

RASFF Alerts – Animal Feed – Salmonella – Aflatoxin

RASFF -Salmonella Lladoff (1 out of 5 samples; 2 out of 5 samples; 3 out of 5 samples /25g) in soybean meal non GMO from Italy in Austria

RASFF -aflatoxins (B1 = 376 / B1 = 356 / B1 = 367 µg/kg – ppb) in groundnuts for birdfeed from Nigeria, via the British Virgin Islands in the UK

USA – Research – What is Salmonella

Food Poisoning BulletinSalmonella

It has long been said that, in 1885, pioneering American veterinary scientist, Daniel E. Salmon, discovered the first strain of Salmonella.  Actually, Theobald Smith, research-assistant to Dr. Salmon, discovered the first strain of Salmonella–Salmonella cholerae suis.  But, being the one in charge, Dr. Salmon got all the credit.  In any case, today the number of known strains of the bacteria totals over two thousand.  In recent years, concerns have been raised, as particular strains of the bacteria have become resistant to traditional antibiotics, in both animals and humans.

USA – USDA Salmonella Reduction Stratergy

FDA FSISUSDA

The U.S. Department of Agriculture’s (USDA) Food Safety and Inspection Service (FSIS) today released its Salmonella Action Plan that outlines the steps it will take to address the most pressing problem it faces–Salmonella in meat and poultry products. An estimated 1.3 million illnesses can be attributed to Salmonella every year.

“Far too many Americans are sickened by Salmonella every year. The aggressive and comprehensive steps detailed in the Salmonella Action Plan will protect consumers by making meat and poultry products safer.” said Under Secretary for Food Safety Elisabeth Hagen.

The Salmonella Action Plan is the agency’s strategy to best address the threat of Salmonella in meat and poultry products.  The plan identifies modernizing the outdated poultry slaughter inspection system as a top priority. By focusing inspectors’ duties solely on food safety, at least 5,000 illnesses can be prevented each year.

 

RASFF Alerts – Sallmonella – Clams – Ice Cream – Pork – Centella – Salami – Turkey

RASFF – Salmonella spp. (presence /25g) in frozen cooked clam (Meretrix lyrata) from Vietnam in Spain

RASFF -Salmonella typhimurium in chilled pork sides from Germany in Norway

RASFF -Salmonella Weltevreden (present /25g) in fresh centella (Centella asiatica) from Sri Lanka in Finland

RASFF -Salmonella spp. (presence /25g) in ham onion salami from Germany

RASFF -Salmonella Saint Paul (presence /25g) in frozen turkey breast from Brazil, via Belgium in Finland

RASFF -Salmonella in ice cream from Serbia in Croatia

RASFF -Salmonella spp. in frozen cooked clams (Meretrix lyrata) from Vietnam in Belgium

RASFF – Salmonella spp. (presence /25g) in frozen cooked clams (Meretrix spp) from Vietnam in Portugal

 

 

 

Norway – 18 Sick Salmonella Outbreak

Global DispatchSalm

The Norwegian Institute of Public Health (NIPH), or the Folkehelseinstituttet reported an outbreak during the month of November caused by a relatively rare strain of the salmonella bacteria, according to a news release this week .

The outbreak of Salmonella Coeln has sickened at least 18 people in several counties (Oslo, Rogaland, Akershus, Nordland Oppland Oslo, Troms, Rogaland, Hordaland, Western College and Østfold) across the country.

USA – Cheese Recall Salmonella

Food Poisoning Bulletin

Flat Creek Farm & Dairy of Georgia is recalling 200 pounds of Heavenly blue cheese because of possible Salmonella enteritidis contamination. As of yesterday, more than 90% of the product has been collected or destroyed. No illnesses have been reported to date.

The cheese is packed in clear plastic and ranges in sizes from 1/2 pound to whole wheels. The lot code is 130912XHB on the front of the package. It was distributed in certain parts of Alabama, Georgia, and Florida and via three online orders, which have all been notified.