Category Archives: Methods

USA – CDC – Multistate Outbreak of E.coli O157:H7 – Spinach

CDCE.coli O157

A total of 28 persons infected with the outbreak strain of Shiga toxin-producing Escherichia coliO157:H7 (STEC O157:H7) have been reported from five states, with most cases in New York.

42% of ill persons have been hospitalized. Two ill persons have developed hemolytic uremic syndrome (HUS), a type of kidney failure, and no deaths have been reported.

The outbreak was initially in New York. More recently, more ill persons in other states have been reported, and the investigation has expanded.

Methods – 3M Clean-TraceTM Water – Total ATP – New Advanced

3M’s Food Safety business has announced its current 3MTM Clean-TraceTM Water – Total ATP test device will move to an advanced, innovative formulation allowing for improved temperature stability and test result accuracy.

The test gives an objective indication of cleanliness in water samples in seconds.  By working in real time, food & beverage producers can rinse test waters after each CIP cycle and re-clean immediately if “fail” results are obtained.

For further information on the new and improved 3MTM Clean-TraceTM Water Plus – Total ATP test please call 01509 613859.

Research – Norovirus and Cleaning

HACCPEUORPA

Noroviruses are a group of viruses that are the most common cause of gastroenteritis (stomach bugs). They are very contagious and can spread easily from person to person. People exposed to the virus usually develop symptoms of illness within 24 to 48 hours, but symptoms can occur as early as 12 hours after exposure.

Common-sense based disinfection guidelines to control norovirus contamination have been developed, but now, for the first time, a Dutch team has come up with science-based guidelines.

In the study the researchers first determined how low the concentration of virions needed to go for transmission to become unlikely. They then tested different methods of cleaning hard surfaces, by using water, soap, or chlorine bleach solutions to determine the best method, or combination of methods for achieving a sufficiently low concentration to prevent virus transmission from hard surfaces, such as your kitchen counters.

The researchers prescribe a two-step process: wipe with a wet cloth, and then disinfect with chlorine. Their prescription is directed at hospitals, schools, restaurants, and other food-handling establishments. In most cases, a 250 ppm solution of chlorine is sufficient, but for high levels of contamination, they recommend 1,000 ppm. To achieve 1,000 ppm, one Suma Tab D4 tablet must be dissolved in 1.5 liters of water (about a quart and a half).

Norovirus is no more resistant to cleaning and disinfection than other pathogens. The virus’ efficiency in causing outbreaks “is more likely due to their extremely low infectious dose,” resulting in the requirement of a very low level of residual contamination in order to prevent further transmission. Fortunately, reducing the level of residual contamination to that low level is not that difficult and can be achieved without extreme measures.

USDA – Revised Microbiology Methods Links

USDA Methods Book

USDA Isolation of Non 0157 Shiga Toxin E.coli with Appendix

USDA Isolation of Listeria monocytogenes with Appendix

USDA Isolation of Listeria monocytogenes PCR