Category Archives: aW

Research – Influence of Hurdle Technology on Foodborne Pathogen Survival in the Human Gastrointestinal Tract

MDPI

Abstract

The application of several sublethal stresses in hurdle technology can exert microbial stress resistance, which, in turn, might enable foodborne pathogens to overcome other types of lethal stresses, such as the gastrointestinal barriers. The present study evaluated the survival of Salmonella Typhimurium and Listeria monocytogenes during simulated digestion, following exposure to combinations of water activity (aw), pH and storage temperature stresses. The results revealed that both pathogens survived their passage through the simulated gastrointestinal tract (GIT) with their previous habituation to certain hurdle combinations inducing stress tolerance. More specifically, the habituation to a low temperature or to a high pH resulted in the increased stress tolerance of Salmonella, while for Listeria, the cells appeared stress tolerant after exposure to a high temperature or to a low pH. Nonetheless, both pathogens expressed increased sensitivity after habituation to growth-limiting hurdle combinations. The survival of stress-tolerant pathogenic cells in the human GIT poses major public health issues, since it can lead to host infection. Consequently, further research is required to obtain a deeper understanding of the adaptive stress responses of foodborne bacteria after exposure to combinations of sublethal hurdles to improve the existing food safety systems.

Research – Spain – Report of the Scientific Committee of the Spanish Agency for Food Safety and Nutrition (AESAN) on the safety of foods air-dried outdoors that are produced by traditional methods and which require an adjustment of the hygiene requirements that must be fulfilled

AESAN

Abstract
The European Union recognises that traditional methods of food production are a valuable and irreplaceable heritage that must be preserved over time. Therefore, Regulation (EC) No. 852/2004 permits a degree of flexibility in its application provided food hygiene goals are not compromised. For decades, both plant and animal-based foods have been produced in Spain which are air-dried outdoors until reaching a low water activity (aw) that enables their preservation at room temperature. These foods include, raisins, dried apricots, dried figs, ñora peppers, dried fish or octopus that are at least partially air-dried, among others.
The Scientific Committee of the Spanish Agency for Food Safety and Nutrition (AESAN) is of the opinion that the processing of naturally dried foods leads to their correct preservation, provided they reach an aw that inhibits the proliferation of pathogenic microorganisms and the production of toxins in them. Some of the stages in the process may cause microbial inactivation.
Above all, it is necessary to ensure the absence of the formation of toxins, with aflatoxins being the ones that have been most frequently identified. Thus, it is considered that the drying should be conducted in the least time possible, ensuring a decrease of aw within the first 2-3 days of below 0.90 to inhibit the development of aflatoxins, and this drying should be continued until aw levels lower than 0.70 are reached, preventing the growth of pathogenic microorganisms that cause spoilage. It is necessary to guarantee suitable hygienic conditions during processing in order to prevent contamination by pathogens and/or toxins.
Although they cannot proliferate in the stated preservation conditions, they can remain viable in the final product, therefore they may pose a risk to consumer health. Microorganisms with a low infective dose and those that have been identified in dried products (such as S. aureus and Salmonella) and microbial toxins are especially relevant. Autonomous Communities must monitor compliance with the requirements to ensure that they do not pose a risk in these products.
Although these types of products have a low aw, within the range of 0.6 to 0.8 according to avail-able literature, given that this information is not available for each assessed product, the level of safety reached cannot be established on an individual basis. Additionally, the diverse factors used in some of them (additives and preservatives, pasteurisation processes, etc.) require individual assessment once all the necessary information is available. Therefore, the drying must reach aw levels below 0.70 in the least time possible for these types of products to be considered stable, as given these conditions, there is no scientific evidence that shows that the safety and stability of the dried products are compromised, provided good hygiene practices are maintained during their preservation and storage.
For final levels of aw that are higher, correct preservation may be achieved through a combination of factors which proves that it is effective throughout the shelf life of the product, maintaining the aforementioned appropriate hygiene practices.