This study determined the antibiotic resistance of the dominant bacteria in the 85 farm BTMs according to the guidelines recommended by the epidemiological cutoff values in the EUCAST. In addition, some physicochemical and microbiological properties of farm BTMs were investigated. The milk samples were divided into two groups according to their SCC values. The milk samples with higher SCC than 400,000 cells mL−1 were further examined bacteriologically, and the antibiotic resistance of isolates was determined. The average TAMB value was 6.34 log CFU/mL in farm BTM. It was found that high-SCC values did not affect other physicochemical properties of BTM samples, such as fat, protein and total solids, except for lactose content. Seventy-two strains were isolated from 45 bulk milk samples. The most prevalent bacteria were Enterococcus spp. (23.61%). The other isolates were Citrobacter spp. (12.5%), Staphylococcus spp. (12.51%), Serratia spp. (11.12%), Klebsiella spp. (9.72%), Bacillus spp. (9.72%), and Enterobacter spp. (8.33%). In antibiotic resistance analysis, 52.6% of Enterobacterales isolates showed cefoxitin resistance, and nine Enterobacterales isolates were determined as the presumptive ESBL producers. None of them was confirmed as ESBL producers. Moreover, MDR was detected in 83.3% of Enterobacter spp. isolates and all Bacillus spp. isolates. The over and inappropriate use of antibiotics in mastitis treatment may cause antibiotic-resistant microorganisms in milk. It was found that 52.7% of the isolated bacteria were MDR, which could pose a risk to public health and food safety, with the consumer’s increasing interest in consuming raw milk.
Posted in Bacillus, Citrobacter, Citrobacter freundii, Decontamination Microbial, Enterobacter cloacae, Food Micro Blog, Food Microbiology, Food Microbiology Blog, Food Microbiology Research, Food Microbiology Testing, Klebsiella, microbial contamination, Microbial growth, Microbiological Risk Assessment, Microbiology, Microbiology Investigations, Microbiology Risk, Raw Milk, Serratia, Staphylococcus aureus
Chili peppers sauce is a dietary complement largely consumed in Cameroon. It is consumed in a powder or wet (pepper sauce) form or directly introduced into cooked food. In this study, the microbiological quality of chili pepper sauce used as food complement in the Buea municipality was assessed. The study was an observational and cross-sectional study involving 70 chili pepper sauce samples from food vendors. The samples were cultured on Salmonella-Shigella agar, violet red bile agar, plate count agar and the colonies isolated were enumerated and identified using the Enterosystem 18R. Factors associated with microbial count were identified using a multiple linear regression model. Bacteria isolate from chili pepper sauce were mainly Entrobacter cloacae (31.57%), Citrobacter freundii (15.78%) and Klebsiella pneumonia (15.78%) and other Enterococcal species. Factors associated with bacteria count were: age of the vendor, number of customers served, types of food and food storage conditions (covering, heating, type of storage containers). Chili pepper sauce used as food complement in Buea Municipality were contaminated with Enteric microorganisms and may represents a potential public health hazard to consumers. The presence of these microorganisms from chilli pepper sauce could result from poor handling.