Research – Prevalence of E. coli , Salmonella , and Listeria spp. as potential pathogens: A comparative study for biofilm of sink drain environment

Wiley Online

Abstract

Since knowledge and understanding of waterborne pathogens and their diseases are well illuminated, a few research publications on the prevalence of pathogenic microorganisms in various household sink drain pipes are often not extensively examined. Therefore, this study aims to (a) assess and monitor the densities of the bacterial community in the different natural biofilm that grow on plastic pipelines, (b) to detect Escherichia coli Salmonella , and Listeria spp. from natural biofilm samples that are collected from the kitchen (= 30), bathroom (= 10), laboratories (= 13), and hospital (= 8) sink drainage pipes. Three bacterial species selected were assessed using a culture‐dependent approach followed by verification of isolates using both BIOLOG GEN III and polymerase chain reaction. The estimated number of each bacterium was 122 isolates, while 60, 20, 26, and 16 isolates were obtained from the natural biofilm samples, kitchen, bathroom, laboratories, and hospital, respectively. As for the tests, in all types of biofilm samples, the overall bacterial counts at low temperature (22°C) were higher than those at high temperature (37°C). Meanwhile, coli had the most significant number of bacterial microorganisms compared to the other two pathogens. Additionally, the most massive cell densities of coli Salmonella , and Listeria species were discovered in the biofilm collected from the kitchen, then the hospital. Statistically, the results reveal that there is a positive correlation (≥ .0001) with significance between the sources of biofilm. This work certainly makes the potential of household sink drain pipes for reservoir contagious pathogens more explicitly noticeable. Such knowledge would also be beneficial for prospective consideration of the threat to human public health and the environment.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s