The first step in quantitative microbial risk assessment (QMRA) is to determine distribution of pathogen contamination among servings of the food at some point in the farm-to-table chain. In the present study, distribution of Salmonella contamination among servings of chicken liver for use in QMRA was determined at meal preparation. A combination of five methods: 1) whole sample enrichment; 2) quantitative polymerase chain reaction; 3) cultural isolation; 4) serotyping; and 5) Monte Carlo simulation were used to determine Salmonella prevalence (P), number (N), and serotype for different serving sizes. In addition, epidemiological data were used to convert serotype data to virulence (V) values for use in QMRA. A Monte Carlo simulation model based in Excel and simulated with @Risk predicted Salmonella P, N, serotype, and V as a function of serving size from one (58 g) to eight (464 g) chicken livers. Salmonella P of chicken livers was 72.5% (58/80) per 58 g. Four serotypes were isolated from chicken livers: 1) Infantis (P = 28%, V = 4.5); 2) Enteritidis (P = 15%, V = 5); 3) Typhimirium (P = 15%, V = 4.8); and 4) Kentucky (P = 15%, V = 0.8). Median Salmonella N was 1.76 log per 58 g (range: 0 to 4.67 log/58 g) and was not affected ( P > 0.05) by serotype. The model predicted a non-linear increase ( P ≤ 0.05) of Salmonella P from 72.5% per 58 g to 100% per 464 g, minimum N from 0 log per 58 g to 1.28 log per 464 g, and median N from 1.76 log per 58 g to 3.22 log per 464 g. Regardless of serving size, predicted maximum N was 4.74 log, mean V was 3.9, and total N was 6.65 log per lot (10,000 chicken livers). The data acquired and model developed in this study fill an important data and modeling gap in QMRA for Salmonella and chicken liver.