Research – Optimization on antimicrobial effects of surfactin against Bacillus cereus spore

JFP

Abstract :The purpose of this study was to establish a three-variable bactericidal model of temperature, time, and concentration to determine the optimal conditions for Bacillus cereus  spore inactivation by surfactin. In order to obtain the binary regression equation of the inactivated spore model, a total of 17 simulations were performed using response surface methodology. The experimental results showed that the three factors each had a discernible but non-equal impact on the inactivation response value. Multiple regression analysis of experimental results using Design-Expert software yielded as the following equation: Optimal inactivation of spores was achieved by treatment with surfactin at a concentration of 4 mg/ml for 40 h at 53 °C, with the response value reaching 1.8. The spores were treated with surfactin under these conditions; the microstructural changes of spores were observed by scanning electron microscopy. We found that the structures of the outer wall of the spores were damaged, while the spores in the control sample showed no visible damage.

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s