Tag Archives: Shiga toxin-producing Escherichia coli

Research – Products – ATCC “Big Six” Non-O157 STEC Reference Standards – Veriflow for E.coli O157:H7 from Invisible Sentinel

Food Safety Connect

ATCC “Big Six” Non-O157 STEC Reference Standards

Verify your quality control assays for the “Big Six” non-O157 Shiga toxin-producing Escherichia coli serogroups classified as adulterants by the USDA. Choose high-quality ATCC cultures tested for Shiga toxin (stx1 and stx2) and intimin (eae) genes from E. coli serogroups O26, O45, O103, O111, O121, and O145.

Food Safety Connect

Veriflow® for E. coli O157:H7 (O157:H7) represents an ultra-sensitive and user-friendly class of diagnostics: Molecular Flow-Based Technology for the detection of E. coli O157:H7 species. With an enrichment time of only 18 hours, Veriflow® O157:H7 can be used for the presumptive and qualitative detection of E. coli O157:H7 from a variety of food matrices. Ultilizing a proprietary multiplexed format, Veriflow® O157:H7 targets multiple conserved genes for E. coli O157:H7 to deliver superior accuracy over current methodologies.

Research – Outbreaks of non-O157 Shiga Toxin-Producing Escherichia coli Infection: USA

Cambridge Journals Ecoli Istock

Non-O157 Shiga toxin-producing Escherichia coli (STEC) infections are increasingly detected, but sources are not well established. We summarize outbreaks to 2010 in the USA. Single-aetiology outbreaks were defined as ≥2 epidemiologically linked culture-confirmed non-O157 STEC infections; multiple-aetiology outbreaks also had laboratory evidence of ≥2 infections caused by another enteric pathogen. Twenty-six states reported 46 outbreaks with 1727 illnesses and 144 hospitalizations. Of 38 single-aetiology outbreaks, 66% were caused by STEC O111 (n = 14) or O26 (n = 11), and 84% were transmitted through food (n = 17) or person-to-person spread (n = 15); food vehicles included dairy products, produce, and meats; childcare centres were the most common setting for person-to-person spread. Of single-aetiology outbreaks, a greater percentage of persons infected by Shiga toxin 2-positive strains had haemolytic uraemic syndrome compared with persons infected by Shiga toxin 1-only positive strains (7% vs. 0·8%). Compared with single-aetiology outbreaks, multiple-aetiology outbreaks were more frequently transmitted through water or animal contact.

 

Research – Europe – Results of surveillance for infections with Shiga toxin-producing Escherichia coli (STEC) of serotype O104:H4 after the large outbreak in Germany, July to December 2011

ECDC ecdclogo

After the massive outbreak of infections with Shiga toxin-producing Escherichia coli (STEC) of serotype O104:H4 in Germany in the summer of 2011, post-outbreak surveillance for further infections with this type of STEC was maintained until the end of 2011. This surveillance was based on national mandatory reporting of STEC infections and the associated complication of haemolytic uraemic syndrome (HUS), as well as on data obtained from a questionnaire. Between the outbreak’s end (5 July) and 31 December 2011, a total of 33 post-outbreak cases were recorded. Post-outbreak cases occurred with diminishing frequency towards the year’s end and resembled the outbreak cases in many respects, however the proportion of HUS among all post-outbreak cases was smaller than during the outbreak. Two thirds of the post-outbreak cases were likely infected by contact with known outbreak cases. Both laboratory and nosocomial spread was noted in this period. No post-outbreak case recalled sprout consumption as a potential source of infection. The scarcity of information conveyed by the nonculture tests routinely used in Germany to diagnose STEC made linkage of post-outbreak cases to the outbreak difficult. Though post-outbreak surveillance demonstrated the outbreak strain’s potential for lengthy chains of transmission aided by prolonged shedding, our results and continued routine surveillance until the end of 2013 do not support the notion, that the outbreak strain has been able to establish itself in the German environment.