Complete genome sequences of Campylobacter coli strains WA333, YF2105, BG2108, MG1116, and BP3183 and Campylobacter jejuni strain IF1100 isolated from retail chicken liver showed the presence of 1,841,551-, 1,687,232-, 1,695,638-, 1,665,146-, 1,695,360-, and 1,744,171-bp circular chromosomes, respectively. These isolates also contained plasmids ranging in size from 5,209 to 55,122 bp.
high prevalence (77%) of Campylobacter jejuni and Campylobacter coli has been reported in retail chicken and beef liver samples (1, 2). A multistate outbreak of C. jejuni associated with undercooked chicken livers occurred in the United States in 2012 (3). A chicken liver-associated foodborne outbreak caused by Campylobacter spp. was reported in Liverpool, United Kingdom (4). Another study in the United Kingdom showed high survival rates of Campylobacter spp. in undercooked chicken livers at restaurants (5). Multilocus sequence typing showed similarities between Campylobacter strains isolated from chicken livers and those causing human illnesses (6, 7). The prevalence of plasmids has been reported to be high in Campylobacter strains isolated from retail chicken liver (8). Other foodborne pathogens, such as Staphylococcus aureus, were also reported to be prevalent in retail beef and chicken livers (9, 10).
Here, we announce the complete genome sequences of five C. coli and one C. jejuni chicken liver isolates. These isolates were previously isolated from retail chicken liver (1). The genomic and plasmid DNA was isolated from a 72-h liquid culture using a DNeasy blood and tissue kit (Qiagen, Inc., Valencia, CA) and plasmid midikit (Qiagen, Inc.). The genome sequencing was performed using the Illumina MiSeq desktop sequencer by applying the Illumina v2 reagent kit. Library preparation was performed using the Nextera XT sample preparation kit (Illumina, Inc., San Diego, CA). Assembly of the chromosomes and plasmids was carried out using CLC genomic workbench and its microbial genome finishing module (Qiagen, Inc.).
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