Monthly Archives: February 2017

Research -Prevalence and risk of heterotrophic microorganisms in a carbonated soft drink factory

Academic Journals

A farm to fork approach was used to establish the prevalence and risk of microorganisms in a carbonated soft drinks factory in south eastern Nigeria. Raw materials, intermediate and finished products were collected from 19 microbiological control points in a processing environment and analysed using membrane filtration over a 12 month period. Yeasts and other heterotrophic bacteria increased during the rainy season but there was no significant difference (p>0.05) in the proliferation of organisms among the analysed months.  Although, organisms were not detected in finished products, there was 100% prevalence for yeasts and bacteria in the processing areas with high sugar activity. No mould, Pseudomonas aeruginosa or Escherichia coli were isolated. Overall, a 5 by 5 risk matrix showed that heterotrophic bacteria, yeasts or mould had low risk of reaching undesirable numbers.  In addition to the prevalence investigation carried out in the processing environment, 864 properly stored bottles of the same brand of carbonated soft drink in trade were purchased from different commercial locations over 12 months and screened. No yeasts mould or coliform bacteria were isolated from the sampling carried out. In conclusion, heterotrophic bacteria and yeasts thrive more on equipment than intermediate or finished products in the process environment studied and the risks of product spoilage or people getting ill was generally low and may  remain so, if good manufacturing and proper after sales handling of the products are observed. Study highlights the need to focus on areas of high sugar activity, to control undesirable organisms and further work is required to establish how microorganisms survive and form biofilms on bottling equipment after sanitation.

RASFF Alert – STEC E.coli -O27 – Ostrich Eye Fillet

kswfoodworld rasff

RASFF – shigatoxin-producing Escherichia coli (stx2+ O27:H30; stx1+ H25) in ostrich eye fillet from South Africa in the Netherlands

RASFF Alerts – Moulds -Sugar Cane – Mandarins

kswfoodworld rasff

RASFF – mandarins from Morocco infested with moulds in Poland

RASFF-spoilage of sugar cane from Egypt infested with moulds in Italy

 

RASFF Alert – Hepatitis A -Chilled Clams

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RASFF – hepatitis A virus (HAV RNA detected /g) in chilled clams (Tapes semidecussatus) from Italy in Italy

RASFF Alerts – Listeria monocytogenes -Chilled Smoked Salmon – Chilled Breaded Pork Foot – Chilled Cooked Beef

kswfoodworld rasff

RASFF – Listeria monocytogenes (<10 CFU/g) in chilled smoked salmon from Poland in the Netherlands

RASFF-Listeria monocytogenes (480 CFU/g) in chilled breaded pork foot from France  in France

RASFF – Listeria monocytogenes (presence /25g) in chilled cooked beef from Belgium in Belgium

RASFF Alerts – Salmonella -Sesame Seeds- Razor Clams

kswfoodworld rasff

RASFF – Salmonella (presence /25g) in sesame seeds from India in the UK

RASFF-Salmonella (presence /25g) in live razor clams (Ensis directus) from the Netherlands in the Netherlands

RASFF Alert – Mycotoxin – Fumonsin -Organic Corn Flour

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RASFF – fumonisins (2660 µg/kg – ppb) in organic corn flour from France in Belgium

RASFF Alerts – Aflatoxin -Pistachios – Groundnuts – Chilli Powder – Peanuts- Hazlenuts

kswfoodworld rasff

RASFF – aflatoxins (B1 = 19.18; Tot. = 21.89 µg/kg – ppb) in shelled pistachios from Iran, via Turkey in Italy

RASFF-aflatoxins (B1 = 5.4 µg/kg – ppb) in groundnuts from China in the UK

RASFF-aflatoxins (B1 = 11.4; Tot. = 12 µg/kg – ppb) in chilli powder from India in Germany

RASFF-aflatoxins (B1 = 29.8; Tot. = 33.2 / B1 = 60.4; Tot. = 70.6 µg/kg – ppb) in peanuts from Egypt in Italy

RASFF-aflatoxins (B1 = 9.1 µg/kg – ppb) in pistachios in shell from Iran in Germany

RASFF-aflatoxins (B1 = 249; Tot. = 279 µg/kg – ppb) in roasted and salted pistachios from Spain in Portugal

RASFF-aflatoxins (B1 = 28.9; Tot. = 32.1 µg/kg – ppb) in pistachios in shell from Iran in Germany

RASFF-aflatoxins (B1 = 17.19; Tot. = 37.31 µg/kg – ppb) in pistachios from Turkey in Germany

RASFF-aflatoxins (B1 = 3.5; Tot. = 4.1 µg/kg – ppb) in blanched peanut kernels from China in Malta

RASFF-aflatoxins (B1 = 23 µg/kg – ppb) in hazelnuts from Azerbaijan in Italy

RASFF-aflatoxins (B1 = 5.5; Tot. = 6.2 / B1 = 7.3; Tot. = 8.1 µg/kg – ppb) in blanched peanuts from China in Greece

RASFF-aflatoxins (B1 = 136; Tot. = 155 µg/kg – ppb) in shelled pistachios from Iran, via Turkey in Italy

 

Research -Trends in carbapenemase-producing Enterobacteriaceae, France, 2012 to 2014

Eurosurveillance

In 2014, a total of 2,976 Enterobacteriaceae isolates with decreased susceptibility to carbapenems were received at the French Associated National Reference Center for Antibiotic Resistance (NRC) and were characterised for their molecular resistance mechanism to carbapenems and compared with results obtained during 2012 and 2013.The overall number of enterobacterial isolates with decreased susceptibility to carbapenems received at the NRC rapidly increased (more than twofold in two years) with a growing proportion of carbapenemase producers (23.1% in 2012 vs 28.6% in 2013 vs 36.2% in 2014). Between 2012 and 2014, the main carbapenemase type was OXA-48, with an increase in OXA-48 variants (mostly OXA-181) and NDM producers, whereas the number KPC producers decreased. We identified a potential spread of OXA-181 producers in the tropical region of Africa. Finally, OXA-48 and OXA-48-related enzymes remained the predominant carbapenemases in France. The number of carbapenemase-producing Escherischia coli isolates was multiplied by fivefold between 2012 and 2014, suggesting a possible dissemination in the community.

Research -Travelers’ diarrhea antibiotics favors particularly resistant super bacteria – ESBL Enterobacteriaceae

EnteroOutbreak News Today

Every year, millions of travelers visit countries with poor hygiene, and approximately one third of them return home carrying antibiotic-resistant ESBL intestinal bacteria. Most of them remain unaware of this, as the bacteria cause no symptoms. High-risk areas for contracting ESBL bacteria are South and South-East Asia, Africa and Latin America.

Diarrhea is the most common health complaint for people who travel to poor regions of the world. Those contracting diarrhea have an increased risk of ESBL acquisition, and if they choose to they treat it with antibiotics, the risk becomes multiplied. A Finnish study led by Professor Anu Kantele and published two years ago showed that among people travelling to high-risk areas, those contracting diarrhea and taking antibiotics, up to 80% brought ESBL super bacteria home with them.